Experiment: ALP with Bacitracin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1868 and Dsui_1869 are separated by 322 nucleotides Dsui_1869 and Dsui_1870 are separated by 130 nucleotides Dsui_1870 and Dsui_1871 overlap by 4 nucleotides
Dsui_1868: Dsui_1868 - HD-GYP domain-containing protein, at 1,964,363 to 1,965,607
_1868
Dsui_1869: Dsui_1869 - hypothetical protein, at 1,965,930 to 1,966,208
_1869
Dsui_1870: Dsui_1870 - efflux transporter, outer membrane factor lipoprotein, NodT family, at 1,966,339 to 1,967,841
_1870
Dsui_1871: Dsui_1871 - hydrophobe/amphiphile efflux-1 (HAE1) family transporter, at 1,967,838 to 1,970,972
_1871
Position (kb)
1966
1967
1968 Strain fitness (log2 ratio)
-2
-1
0
1 at 1965.392 kb on + strand, within Dsui_1868 at 1965.443 kb on - strand at 1965.708 kb on - strand at 1965.712 kb on + strand at 1965.753 kb on - strand at 1965.872 kb on + strand at 1965.920 kb on + strand at 1965.944 kb on + strand at 1965.961 kb on + strand, within Dsui_1869 at 1966.004 kb on + strand, within Dsui_1869 at 1966.083 kb on + strand, within Dsui_1869 at 1966.113 kb on + strand, within Dsui_1869 at 1966.349 kb on + strand at 1966.349 kb on + strand at 1966.432 kb on - strand at 1966.436 kb on + strand at 1966.459 kb on + strand at 1966.468 kb on + strand at 1966.494 kb on + strand, within Dsui_1870 at 1966.564 kb on + strand, within Dsui_1870 at 1966.686 kb on - strand, within Dsui_1870 at 1966.724 kb on + strand, within Dsui_1870 at 1966.724 kb on + strand at 1966.724 kb on + strand, within Dsui_1870 at 1966.732 kb on - strand, within Dsui_1870 at 1966.732 kb on - strand, within Dsui_1870 at 1966.732 kb on - strand, within Dsui_1870 at 1966.734 kb on + strand, within Dsui_1870 at 1966.872 kb on + strand, within Dsui_1870 at 1966.872 kb on + strand, within Dsui_1870 at 1966.880 kb on - strand, within Dsui_1870 at 1966.884 kb on - strand, within Dsui_1870 at 1967.100 kb on + strand, within Dsui_1870 at 1967.116 kb on - strand, within Dsui_1870 at 1967.197 kb on + strand, within Dsui_1870 at 1967.205 kb on - strand, within Dsui_1870 at 1967.265 kb on + strand, within Dsui_1870 at 1967.265 kb on + strand, within Dsui_1870 at 1967.273 kb on - strand, within Dsui_1870 at 1967.273 kb on - strand, within Dsui_1870 at 1967.465 kb on + strand at 1967.492 kb on + strand, within Dsui_1870 at 1967.557 kb on - strand at 1967.567 kb on - strand, within Dsui_1870 at 1967.584 kb on - strand, within Dsui_1870 at 1967.770 kb on + strand at 1967.778 kb on - strand at 1967.811 kb on - strand at 1967.862 kb on - strand at 1967.964 kb on - strand at 1968.141 kb on + strand at 1968.156 kb on - strand, within Dsui_1871 at 1968.353 kb on - strand, within Dsui_1871 at 1968.416 kb on - strand, within Dsui_1871 at 1968.446 kb on + strand, within Dsui_1871 at 1968.490 kb on + strand at 1968.490 kb on + strand, within Dsui_1871 at 1968.522 kb on - strand, within Dsui_1871 at 1968.557 kb on + strand at 1968.565 kb on - strand, within Dsui_1871 at 1968.580 kb on + strand, within Dsui_1871 at 1968.593 kb on - strand, within Dsui_1871 at 1968.613 kb on - strand, within Dsui_1871 at 1968.613 kb on - strand, within Dsui_1871 at 1968.628 kb on + strand, within Dsui_1871 at 1968.629 kb on + strand at 1968.676 kb on + strand, within Dsui_1871 at 1968.725 kb on + strand, within Dsui_1871 at 1968.732 kb on - strand, within Dsui_1871 at 1968.795 kb on - strand, within Dsui_1871 at 1968.824 kb on + strand, within Dsui_1871
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Bacitracin 0.2 mg/ml remove 1,965,392 + Dsui_1868 0.83 -2.3 1,965,443 - -0.5 1,965,708 - -0.4 1,965,712 + +0.1 1,965,753 - +0.6 1,965,872 + +0.3 1,965,920 + +0.3 1,965,944 + -0.1 1,965,961 + Dsui_1869 0.11 +0.1 1,966,004 + Dsui_1869 0.27 +0.4 1,966,083 + Dsui_1869 0.55 +0.3 1,966,113 + Dsui_1869 0.66 +0.3 1,966,349 + -0.7 1,966,349 + +0.4 1,966,432 - -0.8 1,966,436 + +0.1 1,966,459 + +0.3 1,966,468 + -0.4 1,966,494 + Dsui_1870 0.10 -1.2 1,966,564 + Dsui_1870 0.15 -1.1 1,966,686 - Dsui_1870 0.23 -1.3 1,966,724 + Dsui_1870 0.26 +0.6 1,966,724 + +0.6 1,966,724 + Dsui_1870 0.26 +0.6 1,966,732 - Dsui_1870 0.26 -1.0 1,966,732 - Dsui_1870 0.26 -0.4 1,966,732 - Dsui_1870 0.26 -1.1 1,966,734 + Dsui_1870 0.26 -2.2 1,966,872 + Dsui_1870 0.35 -1.0 1,966,872 + Dsui_1870 0.35 -1.4 1,966,880 - Dsui_1870 0.36 -1.3 1,966,884 - Dsui_1870 0.36 +0.2 1,967,100 + Dsui_1870 0.51 -0.4 1,967,116 - Dsui_1870 0.52 +0.8 1,967,197 + Dsui_1870 0.57 -0.4 1,967,205 - Dsui_1870 0.58 -1.5 1,967,265 + Dsui_1870 0.62 +0.6 1,967,265 + Dsui_1870 0.62 -1.0 1,967,273 - Dsui_1870 0.62 +0.3 1,967,273 - Dsui_1870 0.62 +0.1 1,967,465 + +0.6 1,967,492 + Dsui_1870 0.77 +0.6 1,967,557 - +0.6 1,967,567 - Dsui_1870 0.82 -1.8 1,967,584 - Dsui_1870 0.83 +0.2 1,967,770 + +0.6 1,967,778 - -1.4 1,967,811 - -0.4 1,967,862 - -0.0 1,967,964 - +0.6 1,968,141 + -0.4 1,968,156 - Dsui_1871 0.10 +0.2 1,968,353 - Dsui_1871 0.16 +1.6 1,968,416 - Dsui_1871 0.18 -0.6 1,968,446 + Dsui_1871 0.19 -1.4 1,968,490 + -0.4 1,968,490 + Dsui_1871 0.21 -1.4 1,968,522 - Dsui_1871 0.22 -1.0 1,968,557 + +0.6 1,968,565 - Dsui_1871 0.23 -0.4 1,968,580 + Dsui_1871 0.24 +0.6 1,968,593 - Dsui_1871 0.24 -0.8 1,968,613 - Dsui_1871 0.25 +0.6 1,968,613 - Dsui_1871 0.25 -1.0 1,968,628 + Dsui_1871 0.25 -1.0 1,968,629 + +0.6 1,968,676 + Dsui_1871 0.27 -0.4 1,968,725 + Dsui_1871 0.28 -0.4 1,968,732 - Dsui_1871 0.29 -0.8 1,968,795 - Dsui_1871 0.31 -1.2 1,968,824 + Dsui_1871 0.31 -0.4
Or see this region's nucleotide sequence