Experiment: ALP with Bacitracin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1557 and Dsui_1558 are separated by 55 nucleotides Dsui_1558 and Dsui_1559 are separated by 246 nucleotides Dsui_1559 and Dsui_1560 are separated by 151 nucleotides
Dsui_1557: Dsui_1557 - 3-phosphoglycerate kinase, at 1,624,190 to 1,625,374
_1557
Dsui_1558: Dsui_1558 - glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase, at 1,625,430 to 1,626,476
_1558
Dsui_1559: Dsui_1559 - hypothetical protein, at 1,626,723 to 1,627,292
_1559
Dsui_1560: Dsui_1560 - glyceraldehyde-3-phosphate dehydrogenase, type I, at 1,627,444 to 1,628,472
_1560
Position (kb)
1625
1626
1627 Strain fitness (log2 ratio)
-2
-1
0
1 at 1625.435 kb on + strand at 1626.584 kb on + strand at 1626.703 kb on + strand at 1626.705 kb on + strand at 1626.711 kb on - strand at 1626.721 kb on - strand at 1626.761 kb on + strand at 1626.790 kb on + strand, within Dsui_1559 at 1626.819 kb on + strand, within Dsui_1559 at 1626.849 kb on - strand, within Dsui_1559 at 1626.888 kb on + strand, within Dsui_1559 at 1626.893 kb on + strand, within Dsui_1559 at 1626.920 kb on - strand, within Dsui_1559 at 1626.930 kb on + strand, within Dsui_1559 at 1626.959 kb on + strand, within Dsui_1559 at 1627.018 kb on + strand, within Dsui_1559 at 1627.162 kb on + strand, within Dsui_1559 at 1627.203 kb on + strand, within Dsui_1559 at 1627.239 kb on + strand at 1627.246 kb on - strand at 1627.262 kb on + strand at 1627.287 kb on + strand at 1627.314 kb on - strand at 1627.335 kb on + strand at 1627.348 kb on + strand at 1627.362 kb on + strand at 1627.370 kb on - strand at 1627.413 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Bacitracin 0.2 mg/ml remove 1,625,435 + +0.1 1,626,584 + +0.1 1,626,703 + -2.5 1,626,705 + +0.1 1,626,711 - -2.3 1,626,721 - -1.5 1,626,761 + -0.9 1,626,790 + Dsui_1559 0.12 -1.9 1,626,819 + Dsui_1559 0.17 -1.5 1,626,849 - Dsui_1559 0.22 -1.5 1,626,888 + Dsui_1559 0.29 -1.9 1,626,893 + Dsui_1559 0.30 +0.1 1,626,920 - Dsui_1559 0.35 -1.5 1,626,930 + Dsui_1559 0.36 -1.9 1,626,959 + Dsui_1559 0.41 +0.1 1,627,018 + Dsui_1559 0.52 +0.1 1,627,162 + Dsui_1559 0.77 -1.8 1,627,203 + Dsui_1559 0.84 -1.5 1,627,239 + +0.1 1,627,246 - -1.5 1,627,262 + -0.9 1,627,287 + -1.5 1,627,314 - -0.9 1,627,335 + -0.9 1,627,348 + -2.5 1,627,362 + +0.1 1,627,370 - -0.9 1,627,413 + -1.9
Or see this region's nucleotide sequence