Experiment: ALP with Bacitracin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1252 and Dsui_1253 are separated by 120 nucleotides Dsui_1253 and Dsui_1254 are separated by 186 nucleotides Dsui_1254 and Dsui_1255 are separated by 53 nucleotides Dsui_1255 and Dsui_1256 are separated by 4 nucleotides Dsui_1256 and Dsui_1257 are separated by 45 nucleotides
Dsui_1252: Dsui_1252 - hypothetical protein, at 1,314,224 to 1,314,817
_1252
Dsui_1253: Dsui_1253 - peroxiredoxin, at 1,314,938 to 1,315,537
_1253
Dsui_1254: Dsui_1254 - TonB-dependent copper receptor, at 1,315,724 to 1,317,742
_1254
Dsui_1255: Dsui_1255 - mannose-6-phosphate isomerase, at 1,317,796 to 1,318,281
_1255
Dsui_1256: Dsui_1256 - hypothetical protein, at 1,318,286 to 1,318,540
_1256
Dsui_1257: Dsui_1257 - DNA-directed DNA polymerase III PolC, at 1,318,586 to 1,322,041
_1257
Position (kb)
1315
1316
1317
1318 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1314.803 kb on - strand at 1314.837 kb on - strand at 1314.954 kb on + strand at 1314.954 kb on + strand at 1314.964 kb on - strand at 1315.038 kb on - strand, within Dsui_1253 at 1315.038 kb on - strand, within Dsui_1253 at 1315.038 kb on - strand, within Dsui_1253 at 1315.038 kb on - strand at 1315.089 kb on + strand, within Dsui_1253 at 1315.104 kb on + strand, within Dsui_1253 at 1315.148 kb on + strand, within Dsui_1253 at 1315.148 kb on + strand, within Dsui_1253 at 1315.148 kb on + strand, within Dsui_1253 at 1315.156 kb on - strand at 1315.156 kb on - strand, within Dsui_1253 at 1315.156 kb on - strand at 1315.158 kb on + strand, within Dsui_1253 at 1315.166 kb on - strand, within Dsui_1253 at 1315.233 kb on + strand, within Dsui_1253 at 1315.240 kb on + strand, within Dsui_1253 at 1315.366 kb on - strand, within Dsui_1253 at 1315.719 kb on + strand at 1315.737 kb on - strand at 1315.737 kb on - strand at 1315.737 kb on - strand at 1315.751 kb on - strand at 1315.801 kb on - strand at 1315.810 kb on - strand at 1315.812 kb on + strand at 1315.816 kb on + strand at 1315.836 kb on + strand at 1315.846 kb on + strand at 1315.912 kb on + strand at 1315.912 kb on + strand at 1315.912 kb on + strand at 1315.989 kb on + strand, within Dsui_1254 at 1316.120 kb on - strand, within Dsui_1254 at 1316.156 kb on - strand, within Dsui_1254 at 1316.209 kb on - strand, within Dsui_1254 at 1316.224 kb on - strand at 1316.282 kb on + strand, within Dsui_1254 at 1316.339 kb on + strand, within Dsui_1254 at 1316.340 kb on - strand, within Dsui_1254 at 1316.340 kb on - strand, within Dsui_1254 at 1316.359 kb on - strand, within Dsui_1254 at 1316.422 kb on - strand, within Dsui_1254 at 1316.529 kb on - strand, within Dsui_1254 at 1316.542 kb on + strand, within Dsui_1254 at 1316.549 kb on - strand, within Dsui_1254 at 1316.550 kb on + strand, within Dsui_1254 at 1316.557 kb on - strand, within Dsui_1254 at 1316.567 kb on + strand, within Dsui_1254 at 1316.595 kb on - strand, within Dsui_1254 at 1316.597 kb on - strand, within Dsui_1254 at 1316.794 kb on + strand, within Dsui_1254 at 1316.800 kb on + strand, within Dsui_1254 at 1316.808 kb on - strand, within Dsui_1254 at 1316.808 kb on - strand, within Dsui_1254 at 1316.912 kb on - strand, within Dsui_1254 at 1317.037 kb on - strand at 1317.078 kb on - strand, within Dsui_1254 at 1317.084 kb on - strand, within Dsui_1254 at 1317.192 kb on - strand, within Dsui_1254 at 1317.206 kb on + strand, within Dsui_1254 at 1317.214 kb on - strand, within Dsui_1254 at 1317.214 kb on - strand, within Dsui_1254 at 1317.309 kb on - strand, within Dsui_1254 at 1317.328 kb on - strand, within Dsui_1254 at 1317.390 kb on + strand, within Dsui_1254 at 1317.420 kb on - strand, within Dsui_1254 at 1317.427 kb on + strand, within Dsui_1254 at 1317.589 kb on - strand at 1317.589 kb on - strand at 1317.594 kb on - strand at 1317.599 kb on - strand at 1317.619 kb on + strand at 1317.627 kb on - strand at 1317.627 kb on - strand at 1317.645 kb on - strand at 1317.648 kb on + strand at 1317.763 kb on - strand at 1317.773 kb on - strand at 1317.823 kb on + strand at 1317.856 kb on - strand, within Dsui_1255 at 1317.916 kb on + strand, within Dsui_1255 at 1317.928 kb on + strand, within Dsui_1255 at 1317.936 kb on - strand, within Dsui_1255 at 1318.004 kb on - strand, within Dsui_1255 at 1318.025 kb on - strand, within Dsui_1255 at 1318.057 kb on + strand at 1318.083 kb on + strand, within Dsui_1255 at 1318.083 kb on - strand, within Dsui_1255 at 1318.125 kb on - strand, within Dsui_1255 at 1318.140 kb on - strand, within Dsui_1255 at 1318.151 kb on - strand, within Dsui_1255 at 1318.285 kb on - strand at 1318.315 kb on - strand, within Dsui_1256 at 1318.359 kb on - strand, within Dsui_1256 at 1318.488 kb on - strand, within Dsui_1256 at 1318.502 kb on - strand, within Dsui_1256 at 1318.549 kb on + strand at 1318.554 kb on + strand at 1318.557 kb on - strand at 1318.557 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Bacitracin 0.2 mg/ml remove 1,314,803 - +0.0 1,314,837 - -1.6 1,314,954 + -0.4 1,314,954 + +0.0 1,314,964 - +0.0 1,315,038 - Dsui_1253 0.17 +0.0 1,315,038 - Dsui_1253 0.17 -1.6 1,315,038 - Dsui_1253 0.17 -2.0 1,315,038 - +0.0 1,315,089 + Dsui_1253 0.25 -0.5 1,315,104 + Dsui_1253 0.28 -0.2 1,315,148 + Dsui_1253 0.35 -0.3 1,315,148 + Dsui_1253 0.35 -0.2 1,315,148 + Dsui_1253 0.35 -0.7 1,315,156 - +0.0 1,315,156 - Dsui_1253 0.36 -1.6 1,315,156 - +0.0 1,315,158 + Dsui_1253 0.37 -0.5 1,315,166 - Dsui_1253 0.38 -2.3 1,315,233 + Dsui_1253 0.49 -0.2 1,315,240 + Dsui_1253 0.50 -0.1 1,315,366 - Dsui_1253 0.71 -1.0 1,315,719 + -1.2 1,315,737 - -1.6 1,315,737 - -1.0 1,315,737 - -3.2 1,315,751 - +0.0 1,315,801 - +0.0 1,315,810 - +0.0 1,315,812 + -3.2 1,315,816 + -1.0 1,315,836 + -1.3 1,315,846 + -3.2 1,315,912 + +0.0 1,315,912 + -0.3 1,315,912 + -1.0 1,315,989 + Dsui_1254 0.13 -2.8 1,316,120 - Dsui_1254 0.20 -1.0 1,316,156 - Dsui_1254 0.21 -2.3 1,316,209 - Dsui_1254 0.24 -1.6 1,316,224 - +0.0 1,316,282 + Dsui_1254 0.28 -0.5 1,316,339 + Dsui_1254 0.30 -2.0 1,316,340 - Dsui_1254 0.31 -1.6 1,316,340 - Dsui_1254 0.31 +0.0 1,316,359 - Dsui_1254 0.31 -1.0 1,316,422 - Dsui_1254 0.35 +0.0 1,316,529 - Dsui_1254 0.40 -1.6 1,316,542 + Dsui_1254 0.41 -1.0 1,316,549 - Dsui_1254 0.41 -1.6 1,316,550 + Dsui_1254 0.41 -2.2 1,316,557 - Dsui_1254 0.41 -1.0 1,316,567 + Dsui_1254 0.42 -1.8 1,316,595 - Dsui_1254 0.43 -2.8 1,316,597 - Dsui_1254 0.43 +0.0 1,316,794 + Dsui_1254 0.53 -1.2 1,316,800 + Dsui_1254 0.53 -1.0 1,316,808 - Dsui_1254 0.54 +0.0 1,316,808 - Dsui_1254 0.54 +0.0 1,316,912 - Dsui_1254 0.59 -2.8 1,317,037 - +0.0 1,317,078 - Dsui_1254 0.67 -1.6 1,317,084 - Dsui_1254 0.67 +0.0 1,317,192 - Dsui_1254 0.73 -3.4 1,317,206 + Dsui_1254 0.73 -1.6 1,317,214 - Dsui_1254 0.74 -1.0 1,317,214 - Dsui_1254 0.74 -1.0 1,317,309 - Dsui_1254 0.79 -2.3 1,317,328 - Dsui_1254 0.79 +0.0 1,317,390 + Dsui_1254 0.83 -1.6 1,317,420 - Dsui_1254 0.84 -2.0 1,317,427 + Dsui_1254 0.84 -1.2 1,317,589 - +0.0 1,317,589 - -2.3 1,317,594 - -3.0 1,317,599 - +0.0 1,317,619 + +1.6 1,317,627 - -1.0 1,317,627 - -1.0 1,317,645 - -1.8 1,317,648 + -2.0 1,317,763 - -0.1 1,317,773 - -1.5 1,317,823 + -2.0 1,317,856 - Dsui_1255 0.12 -0.1 1,317,916 + Dsui_1255 0.25 -1.0 1,317,928 + Dsui_1255 0.27 -3.3 1,317,936 - Dsui_1255 0.29 -0.6 1,318,004 - Dsui_1255 0.43 -0.5 1,318,025 - Dsui_1255 0.47 -0.3 1,318,057 + +0.0 1,318,083 + Dsui_1255 0.59 -2.0 1,318,083 - Dsui_1255 0.59 -0.3 1,318,125 - Dsui_1255 0.68 -0.5 1,318,140 - Dsui_1255 0.71 -0.5 1,318,151 - Dsui_1255 0.73 -0.5 1,318,285 - +0.4 1,318,315 - Dsui_1256 0.11 -0.3 1,318,359 - Dsui_1256 0.29 -0.8 1,318,488 - Dsui_1256 0.79 -0.4 1,318,502 - Dsui_1256 0.85 -0.2 1,318,549 + -1.6 1,318,554 + -2.0 1,318,557 - -0.6 1,318,557 - +0.4
Or see this region's nucleotide sequence