Experiment: ALP with Bacitracin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0733 and Dsui_0734 overlap by 4 nucleotides Dsui_0734 and Dsui_0735 are separated by 265 nucleotides Dsui_0735 and Dsui_0736 are separated by 93 nucleotides
Dsui_0733: Dsui_0733 - hypothetical protein, at 730,392 to 730,703
_0733
Dsui_0734: Dsui_0734 - acetyltransferase, at 730,700 to 731,239
_0734
Dsui_0735: Dsui_0735 - putative membrane protein, at 731,505 to 731,984
_0735
Dsui_0736: Dsui_0736 - FAD/FMN-dependent dehydrogenase, at 732,078 to 735,956
_0736
Position (kb) 731
732 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 730.524 kb on - strand, within Dsui_0733 at 730.551 kb on + strand, within Dsui_0733 at 730.559 kb on - strand, within Dsui_0733 at 730.607 kb on - strand, within Dsui_0733 at 730.644 kb on + strand, within Dsui_0733 at 730.657 kb on + strand at 730.707 kb on + strand at 730.707 kb on + strand at 730.728 kb on + strand at 730.865 kb on - strand, within Dsui_0734 at 731.030 kb on - strand, within Dsui_0734 at 731.080 kb on - strand, within Dsui_0734 at 731.202 kb on - strand at 731.202 kb on - strand at 731.242 kb on - strand at 731.439 kb on + strand at 731.441 kb on - strand at 731.441 kb on - strand at 731.451 kb on - strand at 731.458 kb on + strand at 731.458 kb on + strand at 731.466 kb on - strand at 731.466 kb on - strand at 731.466 kb on - strand at 731.487 kb on + strand at 731.493 kb on + strand at 731.495 kb on - strand at 731.514 kb on + strand at 731.527 kb on - strand at 731.527 kb on - strand at 731.527 kb on - strand at 731.540 kb on - strand at 731.556 kb on - strand, within Dsui_0735 at 731.567 kb on - strand, within Dsui_0735 at 731.663 kb on - strand, within Dsui_0735 at 731.675 kb on - strand, within Dsui_0735 at 731.749 kb on - strand, within Dsui_0735 at 731.802 kb on - strand, within Dsui_0735 at 731.821 kb on + strand, within Dsui_0735 at 731.835 kb on - strand, within Dsui_0735 at 731.913 kb on - strand, within Dsui_0735 at 731.915 kb on - strand, within Dsui_0735 at 732.000 kb on + strand at 732.007 kb on + strand at 732.008 kb on - strand at 732.014 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Bacitracin 0.2 mg/ml remove 730,524 - Dsui_0733 0.42 -2.9 730,551 + Dsui_0733 0.51 -0.9 730,559 - Dsui_0733 0.54 +0.1 730,607 - Dsui_0733 0.69 +0.1 730,644 + Dsui_0733 0.81 -2.2 730,657 + +0.1 730,707 + +0.1 730,707 + -1.9 730,728 + -3.2 730,865 - Dsui_0734 0.31 -0.9 731,030 - Dsui_0734 0.61 -1.9 731,080 - Dsui_0734 0.70 -0.9 731,202 - +0.1 731,202 - -2.2 731,242 - -1.9 731,439 + -1.5 731,441 - -0.9 731,441 - -0.9 731,451 - -0.9 731,458 + -0.9 731,458 + +0.1 731,466 - +0.1 731,466 - +0.1 731,466 - -1.9 731,487 + -0.9 731,493 + +0.1 731,495 - -0.9 731,514 + -2.2 731,527 - -0.9 731,527 - -2.5 731,527 - -0.9 731,540 - -0.9 731,556 - Dsui_0735 0.11 -1.5 731,567 - Dsui_0735 0.13 -0.9 731,663 - Dsui_0735 0.33 -2.2 731,675 - Dsui_0735 0.35 -0.9 731,749 - Dsui_0735 0.51 -3.4 731,802 - Dsui_0735 0.62 -1.9 731,821 + Dsui_0735 0.66 +0.2 731,835 - Dsui_0735 0.69 -2.5 731,913 - Dsui_0735 0.85 -1.5 731,915 - Dsui_0735 0.85 +0.1 732,000 + -0.6 732,007 + -0.2 732,008 - -1.5 732,014 - -1.9
Or see this region's nucleotide sequence