Experiment: ALP with Bacitracin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0530 and Dsui_0531 overlap by 4 nucleotides Dsui_0531 and Dsui_0532 are separated by 58 nucleotides Dsui_0532 and Dsui_0533 are separated by 116 nucleotides
Dsui_0530: Dsui_0530 - ABC-type Fe3+-siderophore transport system, permease component, at 535,960 to 536,940
_0530
Dsui_0531: Dsui_0531 - ABC-type cobalamin/Fe3+-siderophore transport system, ATPase component, at 536,937 to 537,707
_0531
Dsui_0532: Dsui_0532 - cob(I)alamin adenosyltransferase, at 537,766 to 538,368
_0532
Dsui_0533: Dsui_0533 - cobyrinic acid a,c-diamide synthase, at 538,485 to 539,792
_0533
Position (kb)
536
537
538 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 535.943 kb on + strand at 536.023 kb on - strand at 536.119 kb on + strand, within Dsui_0530 at 536.180 kb on - strand, within Dsui_0530 at 536.236 kb on - strand, within Dsui_0530 at 536.241 kb on - strand, within Dsui_0530 at 536.361 kb on + strand, within Dsui_0530 at 536.451 kb on - strand at 536.467 kb on + strand, within Dsui_0530 at 536.519 kb on - strand, within Dsui_0530 at 536.620 kb on + strand, within Dsui_0530 at 536.628 kb on - strand, within Dsui_0530 at 536.659 kb on - strand, within Dsui_0530 at 536.902 kb on + strand at 536.917 kb on + strand at 536.943 kb on + strand at 536.952 kb on - strand at 536.998 kb on - strand at 537.024 kb on + strand, within Dsui_0531 at 537.064 kb on + strand, within Dsui_0531 at 537.136 kb on + strand, within Dsui_0531 at 537.186 kb on + strand, within Dsui_0531 at 537.291 kb on + strand, within Dsui_0531 at 537.296 kb on + strand, within Dsui_0531 at 537.299 kb on - strand, within Dsui_0531 at 537.304 kb on - strand at 537.321 kb on + strand, within Dsui_0531 at 537.472 kb on + strand, within Dsui_0531 at 537.645 kb on + strand at 537.677 kb on - strand at 537.682 kb on - strand at 537.836 kb on - strand at 537.899 kb on - strand, within Dsui_0532 at 537.899 kb on - strand, within Dsui_0532 at 537.937 kb on - strand, within Dsui_0532 at 538.014 kb on + strand, within Dsui_0532 at 538.053 kb on - strand, within Dsui_0532 at 538.132 kb on - strand, within Dsui_0532 at 538.172 kb on - strand, within Dsui_0532 at 538.212 kb on - strand, within Dsui_0532 at 538.325 kb on + strand at 538.538 kb on + strand at 538.565 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Bacitracin 0.2 mg/ml remove 535,943 + -0.2 536,023 - +0.1 536,119 + Dsui_0530 0.16 -0.5 536,180 - Dsui_0530 0.22 -0.9 536,236 - Dsui_0530 0.28 -2.5 536,241 - Dsui_0530 0.29 -2.5 536,361 + Dsui_0530 0.41 -0.7 536,451 - -0.9 536,467 + Dsui_0530 0.52 -0.4 536,519 - Dsui_0530 0.57 -2.2 536,620 + Dsui_0530 0.67 -0.1 536,628 - Dsui_0530 0.68 +0.1 536,659 - Dsui_0530 0.71 +0.1 536,902 + -0.2 536,917 + -0.3 536,943 + -0.4 536,952 - +0.1 536,998 - -0.9 537,024 + Dsui_0531 0.11 -0.1 537,064 + Dsui_0531 0.16 -2.2 537,136 + Dsui_0531 0.26 -0.4 537,186 + Dsui_0531 0.32 -0.2 537,291 + Dsui_0531 0.46 -0.1 537,296 + Dsui_0531 0.47 -0.3 537,299 - Dsui_0531 0.47 -0.9 537,304 - +0.1 537,321 + Dsui_0531 0.50 -0.4 537,472 + Dsui_0531 0.69 -0.3 537,645 + -0.4 537,677 - -2.7 537,682 - -1.9 537,836 - +0.1 537,899 - Dsui_0532 0.22 -1.2 537,899 - Dsui_0532 0.22 -2.2 537,937 - Dsui_0532 0.28 +0.1 538,014 + Dsui_0532 0.41 -0.9 538,053 - Dsui_0532 0.48 -2.5 538,132 - Dsui_0532 0.61 -3.2 538,172 - Dsui_0532 0.67 +0.1 538,212 - Dsui_0532 0.74 +0.1 538,325 + +0.1 538,538 + -1.5 538,565 + +0.1
Or see this region's nucleotide sequence