Experiment: ALP with Bacitracin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0522 and Dsui_0523 overlap by 1 nucleotides Dsui_0523 and Dsui_0524 are separated by 244 nucleotides Dsui_0524 and Dsui_0525 overlap by 11 nucleotides Dsui_0525 and Dsui_0527 are separated by 288 nucleotides
Dsui_0522: Dsui_0522 - SSS sodium solute transporter, at 529,167 to 530,840
_0522
Dsui_0523: Dsui_0523 - putative membrane protein, at 530,840 to 531,160
_0523
Dsui_0524: Dsui_0524 - putative permease, at 531,405 to 532,208
_0524
Dsui_0525: Dsui_0525 - hypothetical protein, at 532,198 to 532,668
_0525
Dsui_0527: Dsui_0527 - hypothetical protein, at 532,957 to 533,286
_0527
Position (kb) 531
532
533 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 530.441 kb on - strand, within Dsui_0522 at 530.537 kb on - strand, within Dsui_0522 at 530.543 kb on + strand, within Dsui_0522 at 530.550 kb on + strand, within Dsui_0522 at 530.635 kb on + strand, within Dsui_0522 at 530.635 kb on + strand, within Dsui_0522 at 530.685 kb on + strand at 530.813 kb on + strand at 530.862 kb on + strand at 530.944 kb on + strand, within Dsui_0523 at 530.991 kb on + strand, within Dsui_0523 at 531.077 kb on + strand, within Dsui_0523 at 531.155 kb on + strand at 531.163 kb on - strand at 531.165 kb on - strand at 531.168 kb on - strand at 531.210 kb on + strand at 531.217 kb on + strand at 531.217 kb on + strand at 531.222 kb on + strand at 531.224 kb on - strand at 531.225 kb on - strand at 531.230 kb on - strand at 531.260 kb on + strand at 531.329 kb on + strand at 531.329 kb on + strand at 531.492 kb on + strand, within Dsui_0524 at 531.546 kb on - strand, within Dsui_0524 at 531.613 kb on - strand, within Dsui_0524 at 531.626 kb on - strand, within Dsui_0524 at 531.655 kb on - strand, within Dsui_0524 at 531.703 kb on + strand, within Dsui_0524 at 531.704 kb on + strand, within Dsui_0524 at 531.842 kb on + strand, within Dsui_0524 at 531.845 kb on - strand, within Dsui_0524 at 531.925 kb on + strand, within Dsui_0524 at 531.928 kb on - strand, within Dsui_0524 at 531.933 kb on - strand, within Dsui_0524 at 531.952 kb on - strand, within Dsui_0524 at 531.986 kb on + strand, within Dsui_0524 at 532.037 kb on - strand, within Dsui_0524 at 532.077 kb on - strand, within Dsui_0524 at 532.167 kb on + strand at 532.173 kb on + strand at 532.175 kb on - strand at 532.185 kb on - strand at 532.213 kb on - strand at 532.218 kb on + strand at 532.218 kb on - strand at 532.248 kb on + strand, within Dsui_0525 at 532.298 kb on + strand, within Dsui_0525 at 532.312 kb on + strand, within Dsui_0525 at 532.371 kb on + strand, within Dsui_0525 at 532.371 kb on + strand, within Dsui_0525 at 532.429 kb on + strand, within Dsui_0525 at 532.453 kb on + strand, within Dsui_0525 at 532.646 kb on + strand at 532.658 kb on + strand at 532.678 kb on - strand at 532.686 kb on - strand at 532.736 kb on + strand at 532.844 kb on + strand at 532.978 kb on + strand at 533.028 kb on + strand, within Dsui_0527 at 533.068 kb on + strand, within Dsui_0527 at 533.068 kb on + strand, within Dsui_0527 at 533.147 kb on + strand, within Dsui_0527 at 533.152 kb on + strand, within Dsui_0527 at 533.155 kb on - strand, within Dsui_0527
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Bacitracin 0.2 mg/ml remove 530,441 - Dsui_0522 0.76 -2.2 530,537 - Dsui_0522 0.82 -2.0 530,543 + Dsui_0522 0.82 +0.1 530,550 + Dsui_0522 0.83 -1.5 530,635 + Dsui_0522 0.88 +0.1 530,635 + Dsui_0522 0.88 -0.9 530,685 + -1.9 530,813 + -1.5 530,862 + -1.9 530,944 + Dsui_0523 0.32 -2.9 530,991 + Dsui_0523 0.47 +0.1 531,077 + Dsui_0523 0.74 -0.9 531,155 + -0.9 531,163 - -0.9 531,165 - -2.3 531,168 - -1.9 531,210 + +0.1 531,217 + -0.9 531,217 + +0.1 531,222 + -1.5 531,224 - +0.1 531,225 - +0.1 531,230 - -1.9 531,260 + +0.1 531,329 + -1.9 531,329 + -0.9 531,492 + Dsui_0524 0.11 -0.6 531,546 - Dsui_0524 0.18 -0.3 531,613 - Dsui_0524 0.26 -0.3 531,626 - Dsui_0524 0.27 -0.7 531,655 - Dsui_0524 0.31 -0.6 531,703 + Dsui_0524 0.37 -3.8 531,704 + Dsui_0524 0.37 -2.2 531,842 + Dsui_0524 0.54 +0.7 531,845 - Dsui_0524 0.55 -0.3 531,925 + Dsui_0524 0.65 -0.9 531,928 - Dsui_0524 0.65 +0.1 531,933 - Dsui_0524 0.66 -0.2 531,952 - Dsui_0524 0.68 -0.4 531,986 + Dsui_0524 0.72 -0.9 532,037 - Dsui_0524 0.79 -0.4 532,077 - Dsui_0524 0.84 -0.3 532,167 + -1.9 532,173 + -1.2 532,175 - -0.4 532,185 - -0.3 532,213 - -0.5 532,218 + -2.2 532,218 - -0.6 532,248 + Dsui_0525 0.11 +0.1 532,298 + Dsui_0525 0.21 -0.9 532,312 + Dsui_0525 0.24 -2.2 532,371 + Dsui_0525 0.37 +0.1 532,371 + Dsui_0525 0.37 -1.9 532,429 + Dsui_0525 0.49 +0.1 532,453 + Dsui_0525 0.54 -2.5 532,646 + +0.1 532,658 + -0.9 532,678 - -0.2 532,686 - +0.5 532,736 + -1.5 532,844 + -1.9 532,978 + -2.2 533,028 + Dsui_0527 0.22 -0.9 533,068 + Dsui_0527 0.34 -1.5 533,068 + Dsui_0527 0.34 -0.9 533,147 + Dsui_0527 0.58 -1.5 533,152 + Dsui_0527 0.59 +0.1 533,155 - Dsui_0527 0.60 +0.0
Or see this region's nucleotide sequence