Experiment: ALP with Bacitracin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0302 and Dsui_0303 are separated by 7 nucleotides Dsui_0303 and Dsui_0304 are separated by 36 nucleotides Dsui_0304 and Dsui_0305 are separated by 53 nucleotides
Dsui_0302: Dsui_0302 - UbiD family decarboxylase, at 303,503 to 304,978
_0302
Dsui_0303: Dsui_0303 - 4-hydroxybenzoate polyprenyl transferase, proteobacterial, at 304,986 to 305,897
_0303
Dsui_0304: Dsui_0304 - hypothetical protein, at 305,934 to 306,182
_0304
Dsui_0305: Dsui_0305 - transcriptional regulator, at 306,236 to 307,129
_0305
Position (kb) 305
306
307 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 305.033 kb on - strand at 305.992 kb on - strand, within Dsui_0304 at 306.044 kb on + strand at 306.100 kb on - strand, within Dsui_0304 at 306.103 kb on + strand at 306.107 kb on + strand, within Dsui_0304 at 306.185 kb on - strand at 306.259 kb on - strand at 306.286 kb on - strand at 306.323 kb on - strand at 306.333 kb on + strand, within Dsui_0305 at 306.424 kb on + strand, within Dsui_0305 at 306.481 kb on + strand at 306.665 kb on - strand, within Dsui_0305 at 306.692 kb on + strand at 306.715 kb on - strand, within Dsui_0305 at 306.733 kb on + strand, within Dsui_0305 at 306.770 kb on + strand at 306.918 kb on + strand at 306.988 kb on + strand, within Dsui_0305 at 307.077 kb on - strand at 307.109 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Bacitracin 0.2 mg/ml remove 305,033 - +0.1 305,992 - Dsui_0304 0.23 +1.3 306,044 + +0.1 306,100 - Dsui_0304 0.67 -0.1 306,103 + +0.1 306,107 + Dsui_0304 0.69 -2.4 306,185 - +0.0 306,259 - -1.0 306,286 - -0.2 306,323 - -0.6 306,333 + Dsui_0305 0.11 -3.0 306,424 + Dsui_0305 0.21 -1.8 306,481 + +0.2 306,665 - Dsui_0305 0.48 -0.8 306,692 + +0.2 306,715 - Dsui_0305 0.54 -0.3 306,733 + Dsui_0305 0.56 -2.4 306,770 + +0.2 306,918 + -0.8 306,988 + Dsui_0305 0.84 -0.8 307,077 - -0.3 307,109 + +0.2
Or see this region's nucleotide sequence