Experiment: ALP with Bacitracin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0042 and Dsui_0043 are separated by 135 nucleotides Dsui_0043 and Dsui_0044 are separated by 25 nucleotides Dsui_0044 and Dsui_0045 are separated by 6 nucleotides Dsui_0045 and Dsui_0046 are separated by 34 nucleotides
Dsui_0042: Dsui_0042 - Protein of unknown function (DUF2892), at 39,210 to 39,401
_0042
Dsui_0043: Dsui_0043 - cAMP-binding protein, at 39,537 to 40,205
_0043
Dsui_0044: Dsui_0044 - exodeoxyribonuclease III, at 40,231 to 41,004
_0044
Dsui_0045: Dsui_0045 - glutaredoxin-like protein, at 41,011 to 41,520
_0045
Dsui_0046: Dsui_0046 - Zn-dependent oligopeptidase, at 41,555 to 43,636
_0046
Position (kb) 40
41
42 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 39.249 kb on + strand at 39.321 kb on + strand, within Dsui_0042 at 39.352 kb on - strand, within Dsui_0042 at 39.438 kb on - strand at 39.463 kb on - strand at 39.628 kb on + strand at 39.639 kb on - strand, within Dsui_0043 at 39.666 kb on + strand at 39.712 kb on - strand, within Dsui_0043 at 39.914 kb on + strand, within Dsui_0043 at 39.933 kb on + strand, within Dsui_0043 at 39.933 kb on + strand, within Dsui_0043 at 39.941 kb on - strand, within Dsui_0043 at 39.982 kb on + strand at 39.990 kb on - strand, within Dsui_0043 at 40.025 kb on + strand at 40.044 kb on - strand, within Dsui_0043 at 40.093 kb on - strand, within Dsui_0043 at 40.120 kb on + strand, within Dsui_0043 at 40.207 kb on - strand at 40.222 kb on + strand at 40.259 kb on + strand at 40.368 kb on + strand, within Dsui_0044 at 40.376 kb on - strand, within Dsui_0044 at 40.458 kb on + strand at 40.674 kb on + strand, within Dsui_0044 at 40.783 kb on + strand, within Dsui_0044 at 40.873 kb on - strand, within Dsui_0044 at 41.000 kb on - strand at 41.027 kb on + strand at 41.094 kb on - strand, within Dsui_0045 at 41.215 kb on + strand, within Dsui_0045 at 41.274 kb on + strand at 41.279 kb on + strand at 41.324 kb on + strand, within Dsui_0045 at 41.332 kb on - strand, within Dsui_0045 at 41.403 kb on - strand, within Dsui_0045 at 41.459 kb on + strand, within Dsui_0045 at 41.467 kb on - strand, within Dsui_0045 at 41.485 kb on - strand at 41.506 kb on - strand at 41.658 kb on + strand at 41.793 kb on + strand, within Dsui_0046 at 41.825 kb on + strand, within Dsui_0046 at 41.899 kb on - strand, within Dsui_0046 at 41.947 kb on - strand, within Dsui_0046
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Bacitracin 0.2 mg/ml remove 39,249 + +0.4 39,321 + Dsui_0042 0.58 +0.4 39,352 - Dsui_0042 0.74 -0.1 39,438 - -0.0 39,463 - -0.4 39,628 + -0.6 39,639 - Dsui_0043 0.15 +0.2 39,666 + -0.6 39,712 - Dsui_0043 0.26 -0.0 39,914 + Dsui_0043 0.56 +0.4 39,933 + Dsui_0043 0.59 -1.6 39,933 + Dsui_0043 0.59 +0.1 39,941 - Dsui_0043 0.60 +0.2 39,982 + -1.2 39,990 - Dsui_0043 0.68 -0.3 40,025 + +0.4 40,044 - Dsui_0043 0.76 -0.1 40,093 - Dsui_0043 0.83 +0.5 40,120 + Dsui_0043 0.87 +0.4 40,207 - -0.3 40,222 + -0.6 40,259 + -1.6 40,368 + Dsui_0044 0.18 +0.4 40,376 - Dsui_0044 0.19 -0.8 40,458 + +0.4 40,674 + Dsui_0044 0.57 -0.6 40,783 + Dsui_0044 0.71 -2.8 40,873 - Dsui_0044 0.83 -1.3 41,000 - -0.8 41,027 + +0.4 41,094 - Dsui_0045 0.16 -0.1 41,215 + Dsui_0045 0.40 -1.2 41,274 + +0.4 41,279 + +0.4 41,324 + Dsui_0045 0.61 -1.6 41,332 - Dsui_0045 0.63 -0.1 41,403 - Dsui_0045 0.77 +0.1 41,459 + Dsui_0045 0.88 -1.9 41,467 - Dsui_0045 0.89 -0.1 41,485 - +0.0 41,506 - -0.3 41,658 + -1.2 41,793 + Dsui_0046 0.11 -0.6 41,825 + Dsui_0046 0.13 -1.2 41,899 - Dsui_0046 0.17 +0.2 41,947 - Dsui_0046 0.19 +0.1
Or see this region's nucleotide sequence