Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25210

Experiment: Uridine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25200 and BWI76_RS25205 are separated by 54 nucleotidesBWI76_RS25205 and BWI76_RS25210 are separated by 135 nucleotidesBWI76_RS25210 and BWI76_RS25215 overlap by 1 nucleotidesBWI76_RS25215 and BWI76_RS25220 are separated by 18 nucleotides BWI76_RS25200: BWI76_RS25200 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 5,242,406 to 5,243,665 _RS25200 BWI76_RS25205: BWI76_RS25205 - hypothetical protein, at 5,243,720 to 5,243,974 _RS25205 BWI76_RS25210: BWI76_RS25210 - sulfate transporter, at 5,244,110 to 5,244,400 _RS25210 BWI76_RS25215: BWI76_RS25215 - phospholipid-binding protein MlaC, at 5,244,400 to 5,245,035 _RS25215 BWI76_RS25220: BWI76_RS25220 - outer membrane lipid asymmetry maintenance protein MlaD, at 5,245,054 to 5,245,605 _RS25220 Position (kb) 5244 5245Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5243.761 kb on - strand, within BWI76_RS25205at 5244.001 kb on - strandat 5244.019 kb on - strandat 5244.039 kb on - strandat 5244.176 kb on + strand, within BWI76_RS25210at 5244.381 kb on + strandat 5244.440 kb on + strandat 5244.624 kb on + strand, within BWI76_RS25215at 5244.666 kb on - strand, within BWI76_RS25215at 5244.677 kb on - strand, within BWI76_RS25215at 5244.737 kb on - strand, within BWI76_RS25215at 5244.890 kb on + strand, within BWI76_RS25215at 5244.956 kb on - strand, within BWI76_RS25215at 5245.147 kb on + strand, within BWI76_RS25220at 5245.147 kb on - strand, within BWI76_RS25220at 5245.224 kb on + strand, within BWI76_RS25220at 5245.268 kb on + strand, within BWI76_RS25220at 5245.326 kb on + strand, within BWI76_RS25220at 5245.369 kb on - strand, within BWI76_RS25220

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Per-strain Table

Position Strand Gene LocusTag Fraction Uridine (N)
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5,243,761 - BWI76_RS25205 0.16 -0.8
5,244,001 - -1.5
5,244,019 - +0.1
5,244,039 - +0.7
5,244,176 + BWI76_RS25210 0.23 +1.0
5,244,381 + +0.1
5,244,440 + -1.3
5,244,624 + BWI76_RS25215 0.35 -0.4
5,244,666 - BWI76_RS25215 0.42 -0.4
5,244,677 - BWI76_RS25215 0.44 -0.6
5,244,737 - BWI76_RS25215 0.53 -0.3
5,244,890 + BWI76_RS25215 0.77 -3.5
5,244,956 - BWI76_RS25215 0.87 +2.0
5,245,147 + BWI76_RS25220 0.17 -1.9
5,245,147 - BWI76_RS25220 0.17 -0.5
5,245,224 + BWI76_RS25220 0.31 +2.2
5,245,268 + BWI76_RS25220 0.39 -0.5
5,245,326 + BWI76_RS25220 0.49 +0.7
5,245,369 - BWI76_RS25220 0.57 +1.7

Or see this region's nucleotide sequence