Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04560

Experiment: Uridine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04555 and BWI76_RS04560 are separated by 168 nucleotidesBWI76_RS04560 and BWI76_RS04565 are separated by 81 nucleotidesBWI76_RS04565 and BWI76_RS04570 are separated by 12 nucleotides BWI76_RS04555: BWI76_RS04555 - citrate/acetate antiporter, at 943,966 to 945,321 _RS04555 BWI76_RS04560: BWI76_RS04560 - holo-ACP synthase CitX, at 945,490 to 946,029 _RS04560 BWI76_RS04565: BWI76_RS04565 - bifunctional tRNA pseudouridine(32) synthase/ribosomal large subunit pseudouridine synthase RluA, at 946,111 to 946,770 _RS04565 BWI76_RS04570: BWI76_RS04570 - RNA polymerase-associated protein RapA, at 946,783 to 949,689 _RS04570 Position (kb) 945 946 947Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 944.665 kb on - strand, within BWI76_RS04555at 944.872 kb on - strand, within BWI76_RS04555at 944.905 kb on + strand, within BWI76_RS04555at 944.910 kb on + strand, within BWI76_RS04555at 944.910 kb on + strand, within BWI76_RS04555at 944.919 kb on + strand, within BWI76_RS04555at 944.970 kb on - strand, within BWI76_RS04555at 944.983 kb on + strand, within BWI76_RS04555at 944.991 kb on - strand, within BWI76_RS04555at 944.991 kb on - strand, within BWI76_RS04555at 944.991 kb on - strand, within BWI76_RS04555at 945.058 kb on - strand, within BWI76_RS04555at 945.058 kb on - strand, within BWI76_RS04555at 945.072 kb on + strand, within BWI76_RS04555at 945.187 kb on - strandat 945.525 kb on - strandat 945.626 kb on - strand, within BWI76_RS04560at 945.644 kb on + strand, within BWI76_RS04560at 945.773 kb on - strand, within BWI76_RS04560at 945.809 kb on - strand, within BWI76_RS04560at 946.053 kb on - strandat 946.098 kb on - strandat 946.108 kb on - strandat 946.342 kb on + strand, within BWI76_RS04565at 946.398 kb on + strand, within BWI76_RS04565at 946.407 kb on + strand, within BWI76_RS04565at 946.474 kb on - strand, within BWI76_RS04565at 946.543 kb on + strand, within BWI76_RS04565at 946.554 kb on + strand, within BWI76_RS04565

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Per-strain Table

Position Strand Gene LocusTag Fraction Uridine (N)
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944,665 - BWI76_RS04555 0.52 -0.3
944,872 - BWI76_RS04555 0.67 +0.1
944,905 + BWI76_RS04555 0.69 -1.9
944,910 + BWI76_RS04555 0.70 -3.5
944,910 + BWI76_RS04555 0.70 -0.4
944,919 + BWI76_RS04555 0.70 -0.3
944,970 - BWI76_RS04555 0.74 +0.3
944,983 + BWI76_RS04555 0.75 +0.3
944,991 - BWI76_RS04555 0.76 +0.4
944,991 - BWI76_RS04555 0.76 +1.1
944,991 - BWI76_RS04555 0.76 -0.3
945,058 - BWI76_RS04555 0.81 +0.4
945,058 - BWI76_RS04555 0.81 -0.1
945,072 + BWI76_RS04555 0.82 +0.2
945,187 - -0.5
945,525 - -0.0
945,626 - BWI76_RS04560 0.25 -0.4
945,644 + BWI76_RS04560 0.29 -0.8
945,773 - BWI76_RS04560 0.52 -1.7
945,809 - BWI76_RS04560 0.59 -2.3
946,053 - +0.8
946,098 - +1.0
946,108 - -4.2
946,342 + BWI76_RS04565 0.35 -1.1
946,398 + BWI76_RS04565 0.43 -0.6
946,407 + BWI76_RS04565 0.45 -1.1
946,474 - BWI76_RS04565 0.55 -0.5
946,543 + BWI76_RS04565 0.65 -0.8
946,554 + BWI76_RS04565 0.67 -0.7

Or see this region's nucleotide sequence