Experiment: L-Methionine and L-Threonine as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0128 and DvMF_0129 are separated by 192 nucleotides DvMF_0129 and DvMF_0130 are separated by 58 nucleotides DvMF_0130 and DvMF_0131 are separated by 360 nucleotides DvMF_0131 and DvMF_0132 are separated by 2 nucleotides
DvMF_0128: DvMF_0128 - multiple antibiotic resistance (MarC)-related protein (RefSeq), at 142,355 to 142,972
_0128
DvMF_0129: DvMF_0129 - 6-pyruvoyl tetrahydropterin synthase and hypothetical protein (RefSeq), at 143,165 to 143,557
_0129
DvMF_0130: DvMF_0130 - DNA polymerase III, alpha subunit (RefSeq), at 143,616 to 147,137
_0130
DvMF_0131: DvMF_0131 - hypothetical protein (RefSeq), at 147,498 to 147,839
_0131
DvMF_0132: DvMF_0132 - metal-dependent phosphohydrolase HD region (RefSeq), at 147,842 to 148,711
_0132
Position (kb) 143
144
145
146
147
148 Strain fitness (log2 ratio) -1
0
1
2
3 at 142.741 kb on + strand, within DvMF_0128 at 142.847 kb on + strand, within DvMF_0128 at 147.377 kb on + strand at 147.447 kb on + strand at 147.675 kb on + strand, within DvMF_0131 at 147.795 kb on - strand, within DvMF_0131 at 147.882 kb on - strand at 148.010 kb on + strand, within DvMF_0132 at 148.019 kb on + strand, within DvMF_0132 at 148.048 kb on - strand, within DvMF_0132 at 148.073 kb on - strand, within DvMF_0132
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine and L-Threonine as nutrient remove 142,741 + DvMF_0128 0.62 +0.3 142,847 + DvMF_0128 0.80 +0.9 147,377 + +0.9 147,447 + +0.2 147,675 + DvMF_0131 0.52 +0.9 147,795 - DvMF_0131 0.87 +3.0 147,882 - +0.1 148,010 + DvMF_0132 0.19 +0.5 148,019 + DvMF_0132 0.20 +1.7 148,048 - DvMF_0132 0.24 +0.3 148,073 - DvMF_0132 0.27 -0.5
Or see this region's nucleotide sequence