Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS24685 and PFLU_RS24690 are separated by 287 nucleotides PFLU_RS24690 and PFLU_RS24695 are separated by 63 nucleotides PFLU_RS24695 and PFLU_RS24700 are separated by 2 nucleotides
PFLU_RS24685: PFLU_RS24685 - phosphoribosylformylglycinamidine synthase, at 5,526,150 to 5,530,046
_RS24685
PFLU_RS24690: PFLU_RS24690 - membrane-bound lytic murein transglycosylase MltF, at 5,530,334 to 5,531,794
_RS24690
PFLU_RS24695: PFLU_RS24695 - PTS glucose transporter subunit IIB, at 5,531,858 to 5,532,136
_RS24695
PFLU_RS24700: PFLU_RS24700 - phosphoenolpyruvate--protein phosphotransferase, at 5,532,139 to 5,534,655
_RS24700
Position (kb) 5530
5531
5532 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5529.820 kb on - strand at 5530.094 kb on + strand at 5530.094 kb on + strand at 5530.094 kb on + strand at 5530.095 kb on - strand at 5530.095 kb on - strand at 5530.099 kb on + strand at 5530.099 kb on + strand at 5530.100 kb on - strand at 5530.100 kb on - strand at 5530.164 kb on + strand at 5530.164 kb on + strand at 5530.164 kb on + strand at 5530.165 kb on - strand at 5530.165 kb on - strand at 5530.165 kb on - strand at 5530.185 kb on + strand at 5530.185 kb on + strand at 5530.185 kb on + strand at 5530.185 kb on + strand at 5530.185 kb on + strand at 5530.186 kb on - strand at 5530.186 kb on - strand at 5530.186 kb on - strand at 5530.186 kb on - strand at 5530.186 kb on - strand at 5530.186 kb on - strand at 5530.206 kb on - strand at 5530.251 kb on - strand at 5530.251 kb on - strand at 5530.273 kb on - strand at 5530.322 kb on + strand at 5530.322 kb on + strand at 5530.322 kb on + strand at 5530.322 kb on + strand at 5530.323 kb on - strand at 5530.323 kb on - strand at 5530.323 kb on - strand at 5530.324 kb on + strand at 5530.324 kb on + strand at 5530.325 kb on - strand at 5530.325 kb on - strand at 5530.420 kb on + strand at 5530.421 kb on - strand at 5530.421 kb on - strand at 5530.458 kb on + strand at 5530.459 kb on - strand at 5530.493 kb on + strand, within PFLU_RS24690 at 5530.493 kb on + strand, within PFLU_RS24690 at 5530.493 kb on + strand, within PFLU_RS24690 at 5530.493 kb on + strand, within PFLU_RS24690 at 5530.493 kb on + strand, within PFLU_RS24690 at 5530.493 kb on + strand, within PFLU_RS24690 at 5530.493 kb on + strand, within PFLU_RS24690 at 5530.494 kb on - strand, within PFLU_RS24690 at 5530.494 kb on - strand, within PFLU_RS24690 at 5530.530 kb on - strand, within PFLU_RS24690 at 5530.686 kb on + strand, within PFLU_RS24690 at 5530.686 kb on + strand, within PFLU_RS24690 at 5530.686 kb on + strand, within PFLU_RS24690 at 5530.686 kb on + strand, within PFLU_RS24690 at 5530.687 kb on - strand, within PFLU_RS24690 at 5530.687 kb on - strand, within PFLU_RS24690 at 5530.687 kb on - strand, within PFLU_RS24690 at 5530.687 kb on - strand, within PFLU_RS24690 at 5530.703 kb on + strand, within PFLU_RS24690 at 5530.703 kb on + strand, within PFLU_RS24690 at 5530.703 kb on + strand, within PFLU_RS24690 at 5530.704 kb on - strand, within PFLU_RS24690 at 5530.704 kb on - strand, within PFLU_RS24690 at 5530.704 kb on - strand, within PFLU_RS24690 at 5530.704 kb on - strand, within PFLU_RS24690 at 5530.704 kb on - strand, within PFLU_RS24690 at 5530.730 kb on + strand, within PFLU_RS24690 at 5530.730 kb on + strand, within PFLU_RS24690 at 5530.731 kb on - strand, within PFLU_RS24690 at 5530.731 kb on - strand, within PFLU_RS24690 at 5530.731 kb on - strand, within PFLU_RS24690 at 5530.731 kb on - strand, within PFLU_RS24690 at 5530.731 kb on - strand, within PFLU_RS24690 at 5530.731 kb on - strand, within PFLU_RS24690 at 5530.731 kb on - strand, within PFLU_RS24690 at 5530.731 kb on - strand, within PFLU_RS24690 at 5530.832 kb on - strand, within PFLU_RS24690 at 5530.856 kb on + strand, within PFLU_RS24690 at 5530.856 kb on + strand, within PFLU_RS24690 at 5530.857 kb on - strand, within PFLU_RS24690 at 5530.857 kb on - strand, within PFLU_RS24690 at 5530.964 kb on + strand, within PFLU_RS24690 at 5530.964 kb on + strand, within PFLU_RS24690 at 5530.964 kb on + strand, within PFLU_RS24690 at 5530.964 kb on + strand, within PFLU_RS24690 at 5530.964 kb on + strand, within PFLU_RS24690 at 5530.964 kb on + strand, within PFLU_RS24690 at 5530.964 kb on + strand, within PFLU_RS24690 at 5530.964 kb on + strand, within PFLU_RS24690 at 5530.965 kb on - strand, within PFLU_RS24690 at 5530.965 kb on - strand, within PFLU_RS24690 at 5530.965 kb on - strand, within PFLU_RS24690 at 5530.965 kb on - strand, within PFLU_RS24690 at 5530.965 kb on - strand, within PFLU_RS24690 at 5530.965 kb on - strand, within PFLU_RS24690 at 5530.965 kb on - strand, within PFLU_RS24690 at 5530.965 kb on - strand, within PFLU_RS24690 at 5530.965 kb on - strand, within PFLU_RS24690 at 5530.965 kb on - strand, within PFLU_RS24690 at 5530.965 kb on - strand, within PFLU_RS24690 at 5530.965 kb on - strand, within PFLU_RS24690 at 5530.965 kb on - strand, within PFLU_RS24690 at 5531.069 kb on + strand, within PFLU_RS24690 at 5531.069 kb on + strand, within PFLU_RS24690 at 5531.069 kb on + strand, within PFLU_RS24690 at 5531.070 kb on - strand, within PFLU_RS24690 at 5531.070 kb on - strand, within PFLU_RS24690 at 5531.070 kb on - strand, within PFLU_RS24690 at 5531.070 kb on - strand, within PFLU_RS24690 at 5531.121 kb on - strand, within PFLU_RS24690 at 5531.124 kb on - strand, within PFLU_RS24690 at 5531.147 kb on + strand, within PFLU_RS24690 at 5531.148 kb on - strand, within PFLU_RS24690 at 5531.148 kb on - strand, within PFLU_RS24690 at 5531.159 kb on + strand, within PFLU_RS24690 at 5531.159 kb on + strand, within PFLU_RS24690 at 5531.160 kb on - strand, within PFLU_RS24690 at 5531.160 kb on - strand, within PFLU_RS24690 at 5531.199 kb on - strand, within PFLU_RS24690 at 5531.199 kb on - strand, within PFLU_RS24690 at 5531.219 kb on + strand, within PFLU_RS24690 at 5531.219 kb on + strand, within PFLU_RS24690 at 5531.219 kb on + strand, within PFLU_RS24690 at 5531.219 kb on + strand, within PFLU_RS24690 at 5531.220 kb on - strand, within PFLU_RS24690 at 5531.220 kb on - strand, within PFLU_RS24690 at 5531.220 kb on - strand, within PFLU_RS24690 at 5531.408 kb on + strand, within PFLU_RS24690 at 5531.408 kb on + strand, within PFLU_RS24690 at 5531.408 kb on + strand, within PFLU_RS24690 at 5531.408 kb on + strand, within PFLU_RS24690 at 5531.408 kb on + strand, within PFLU_RS24690 at 5531.408 kb on + strand, within PFLU_RS24690 at 5531.408 kb on + strand, within PFLU_RS24690 at 5531.408 kb on + strand, within PFLU_RS24690 at 5531.408 kb on + strand, within PFLU_RS24690 at 5531.408 kb on + strand, within PFLU_RS24690 at 5531.408 kb on + strand, within PFLU_RS24690 at 5531.408 kb on + strand, within PFLU_RS24690 at 5531.409 kb on - strand, within PFLU_RS24690 at 5531.409 kb on - strand, within PFLU_RS24690 at 5531.409 kb on - strand, within PFLU_RS24690 at 5531.409 kb on - strand, within PFLU_RS24690 at 5531.409 kb on - strand, within PFLU_RS24690 at 5531.409 kb on - strand, within PFLU_RS24690 at 5531.409 kb on - strand, within PFLU_RS24690 at 5531.409 kb on - strand, within PFLU_RS24690 at 5531.409 kb on - strand, within PFLU_RS24690 at 5531.409 kb on - strand, within PFLU_RS24690 at 5531.483 kb on + strand, within PFLU_RS24690 at 5531.483 kb on + strand, within PFLU_RS24690 at 5531.483 kb on + strand, within PFLU_RS24690 at 5531.484 kb on - strand, within PFLU_RS24690 at 5531.484 kb on - strand, within PFLU_RS24690 at 5531.603 kb on + strand, within PFLU_RS24690 at 5531.603 kb on + strand, within PFLU_RS24690 at 5531.603 kb on + strand, within PFLU_RS24690 at 5531.604 kb on - strand, within PFLU_RS24690 at 5531.604 kb on - strand, within PFLU_RS24690 at 5531.604 kb on - strand, within PFLU_RS24690 at 5531.624 kb on + strand, within PFLU_RS24690 at 5531.624 kb on + strand, within PFLU_RS24690 at 5531.625 kb on - strand, within PFLU_RS24690 at 5531.672 kb on + strand at 5531.672 kb on + strand at 5531.672 kb on + strand at 5531.672 kb on + strand at 5531.672 kb on + strand at 5531.673 kb on - strand at 5531.675 kb on + strand at 5531.675 kb on + strand at 5531.675 kb on + strand at 5531.675 kb on + strand at 5531.675 kb on + strand at 5531.675 kb on + strand at 5531.676 kb on - strand at 5531.676 kb on - strand at 5531.676 kb on - strand at 5531.764 kb on + strand at 5531.764 kb on + strand at 5531.764 kb on + strand at 5531.765 kb on - strand at 5531.779 kb on + strand at 5531.780 kb on - strand at 5531.780 kb on - strand at 5531.792 kb on + strand at 5531.792 kb on + strand at 5531.792 kb on + strand at 5531.792 kb on + strand at 5531.792 kb on + strand at 5531.792 kb on + strand at 5531.792 kb on + strand at 5531.792 kb on + strand at 5531.792 kb on + strand at 5531.792 kb on + strand at 5531.793 kb on - strand at 5531.793 kb on - strand at 5531.793 kb on - strand at 5531.793 kb on - strand at 5531.793 kb on - strand at 5531.859 kb on + strand at 5531.860 kb on - strand at 5531.860 kb on - strand at 5531.860 kb on - strand at 5531.882 kb on + strand at 5531.882 kb on + strand at 5531.883 kb on - strand at 5532.010 kb on + strand, within PFLU_RS24695 at 5532.123 kb on - strand at 5532.123 kb on - strand at 5532.182 kb on + strand at 5532.183 kb on - strand at 5532.183 kb on - strand at 5532.509 kb on + strand, within PFLU_RS24700 at 5532.509 kb on + strand, within PFLU_RS24700
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs remove 5,529,820 - +0.8 5,530,094 + -1.8 5,530,094 + -1.2 5,530,094 + -0.2 5,530,095 - -2.5 5,530,095 - -1.2 5,530,099 + -0.1 5,530,099 + -0.8 5,530,100 - -1.8 5,530,100 - +2.0 5,530,164 + -1.7 5,530,164 + -2.1 5,530,164 + -0.2 5,530,165 - +0.2 5,530,165 - -0.3 5,530,165 - +0.3 5,530,185 + -0.4 5,530,185 + -2.0 5,530,185 + -1.1 5,530,185 + -0.6 5,530,185 + -2.4 5,530,186 - +1.2 5,530,186 - +2.0 5,530,186 - +2.9 5,530,186 - -3.1 5,530,186 - +0.1 5,530,186 - -2.1 5,530,206 - -1.6 5,530,251 - +2.1 5,530,251 - -0.6 5,530,273 - +0.8 5,530,322 + +1.2 5,530,322 + -0.0 5,530,322 + -1.7 5,530,322 + -0.8 5,530,323 - -0.6 5,530,323 - -1.0 5,530,323 - +0.7 5,530,324 + -0.3 5,530,324 + -1.4 5,530,325 - -2.2 5,530,325 - +1.2 5,530,420 + -3.3 5,530,421 - -0.9 5,530,421 - -1.4 5,530,458 + -0.4 5,530,459 - -1.7 5,530,493 + PFLU_RS24690 0.11 -1.0 5,530,493 + PFLU_RS24690 0.11 -2.2 5,530,493 + PFLU_RS24690 0.11 +2.4 5,530,493 + PFLU_RS24690 0.11 -1.1 5,530,493 + PFLU_RS24690 0.11 -2.3 5,530,493 + PFLU_RS24690 0.11 -0.8 5,530,493 + PFLU_RS24690 0.11 -1.2 5,530,494 - PFLU_RS24690 0.11 -1.2 5,530,494 - PFLU_RS24690 0.11 -0.4 5,530,530 - PFLU_RS24690 0.13 -0.6 5,530,686 + PFLU_RS24690 0.24 -3.0 5,530,686 + PFLU_RS24690 0.24 -1.2 5,530,686 + PFLU_RS24690 0.24 -2.3 5,530,686 + PFLU_RS24690 0.24 +0.3 5,530,687 - PFLU_RS24690 0.24 +0.3 5,530,687 - PFLU_RS24690 0.24 -0.1 5,530,687 - PFLU_RS24690 0.24 -2.0 5,530,687 - PFLU_RS24690 0.24 -1.4 5,530,703 + PFLU_RS24690 0.25 -0.8 5,530,703 + PFLU_RS24690 0.25 +0.9 5,530,703 + PFLU_RS24690 0.25 -2.6 5,530,704 - PFLU_RS24690 0.25 -1.4 5,530,704 - PFLU_RS24690 0.25 -1.6 5,530,704 - PFLU_RS24690 0.25 -1.8 5,530,704 - PFLU_RS24690 0.25 +0.9 5,530,704 - PFLU_RS24690 0.25 -1.6 5,530,730 + PFLU_RS24690 0.27 +0.9 5,530,730 + PFLU_RS24690 0.27 -1.9 5,530,731 - PFLU_RS24690 0.27 -2.4 5,530,731 - PFLU_RS24690 0.27 -0.6 5,530,731 - PFLU_RS24690 0.27 -0.3 5,530,731 - PFLU_RS24690 0.27 -0.2 5,530,731 - PFLU_RS24690 0.27 -2.8 5,530,731 - PFLU_RS24690 0.27 -0.1 5,530,731 - PFLU_RS24690 0.27 -2.7 5,530,731 - PFLU_RS24690 0.27 -1.8 5,530,832 - PFLU_RS24690 0.34 -2.1 5,530,856 + PFLU_RS24690 0.36 -2.8 5,530,856 + PFLU_RS24690 0.36 -0.0 5,530,857 - PFLU_RS24690 0.36 +0.5 5,530,857 - PFLU_RS24690 0.36 -4.1 5,530,964 + PFLU_RS24690 0.43 -0.8 5,530,964 + PFLU_RS24690 0.43 -2.7 5,530,964 + PFLU_RS24690 0.43 -2.1 5,530,964 + PFLU_RS24690 0.43 -2.6 5,530,964 + PFLU_RS24690 0.43 -3.6 5,530,964 + PFLU_RS24690 0.43 -1.6 5,530,964 + PFLU_RS24690 0.43 -2.1 5,530,964 + PFLU_RS24690 0.43 +0.8 5,530,965 - PFLU_RS24690 0.43 -1.0 5,530,965 - PFLU_RS24690 0.43 -3.0 5,530,965 - PFLU_RS24690 0.43 +0.8 5,530,965 - PFLU_RS24690 0.43 -2.8 5,530,965 - PFLU_RS24690 0.43 +2.1 5,530,965 - PFLU_RS24690 0.43 -3.5 5,530,965 - PFLU_RS24690 0.43 +0.6 5,530,965 - PFLU_RS24690 0.43 -0.8 5,530,965 - PFLU_RS24690 0.43 -0.2 5,530,965 - PFLU_RS24690 0.43 +0.8 5,530,965 - PFLU_RS24690 0.43 -3.0 5,530,965 - PFLU_RS24690 0.43 -2.2 5,530,965 - PFLU_RS24690 0.43 -2.4 5,531,069 + PFLU_RS24690 0.50 +2.5 5,531,069 + PFLU_RS24690 0.50 -2.0 5,531,069 + PFLU_RS24690 0.50 -1.4 5,531,070 - PFLU_RS24690 0.50 -2.8 5,531,070 - PFLU_RS24690 0.50 -2.8 5,531,070 - PFLU_RS24690 0.50 -1.9 5,531,070 - PFLU_RS24690 0.50 -2.3 5,531,121 - PFLU_RS24690 0.54 -2.0 5,531,124 - PFLU_RS24690 0.54 -4.2 5,531,147 + PFLU_RS24690 0.56 -2.3 5,531,148 - PFLU_RS24690 0.56 -0.9 5,531,148 - PFLU_RS24690 0.56 -3.3 5,531,159 + PFLU_RS24690 0.56 -1.7 5,531,159 + PFLU_RS24690 0.56 -1.6 5,531,160 - PFLU_RS24690 0.57 -1.4 5,531,160 - PFLU_RS24690 0.57 -2.6 5,531,199 - PFLU_RS24690 0.59 -1.7 5,531,199 - PFLU_RS24690 0.59 +0.2 5,531,219 + PFLU_RS24690 0.61 -0.7 5,531,219 + PFLU_RS24690 0.61 -1.8 5,531,219 + PFLU_RS24690 0.61 -2.1 5,531,219 + PFLU_RS24690 0.61 -1.6 5,531,220 - PFLU_RS24690 0.61 +0.6 5,531,220 - PFLU_RS24690 0.61 -2.0 5,531,220 - PFLU_RS24690 0.61 -0.4 5,531,408 + PFLU_RS24690 0.74 -1.8 5,531,408 + PFLU_RS24690 0.74 +0.2 5,531,408 + PFLU_RS24690 0.74 -2.3 5,531,408 + PFLU_RS24690 0.74 -0.6 5,531,408 + PFLU_RS24690 0.74 -1.4 5,531,408 + PFLU_RS24690 0.74 +2.1 5,531,408 + PFLU_RS24690 0.74 -1.1 5,531,408 + PFLU_RS24690 0.74 -3.0 5,531,408 + PFLU_RS24690 0.74 -3.5 5,531,408 + PFLU_RS24690 0.74 -1.9 5,531,408 + PFLU_RS24690 0.74 -1.7 5,531,408 + PFLU_RS24690 0.74 -0.4 5,531,409 - PFLU_RS24690 0.74 -2.7 5,531,409 - PFLU_RS24690 0.74 -1.6 5,531,409 - PFLU_RS24690 0.74 +1.5 5,531,409 - PFLU_RS24690 0.74 -0.9 5,531,409 - PFLU_RS24690 0.74 -1.3 5,531,409 - PFLU_RS24690 0.74 -1.8 5,531,409 - PFLU_RS24690 0.74 -1.6 5,531,409 - PFLU_RS24690 0.74 -0.6 5,531,409 - PFLU_RS24690 0.74 -2.8 5,531,409 - PFLU_RS24690 0.74 -1.1 5,531,483 + PFLU_RS24690 0.79 -2.6 5,531,483 + PFLU_RS24690 0.79 +1.0 5,531,483 + PFLU_RS24690 0.79 -0.6 5,531,484 - PFLU_RS24690 0.79 -1.0 5,531,484 - PFLU_RS24690 0.79 -1.2 5,531,603 + PFLU_RS24690 0.87 -0.2 5,531,603 + PFLU_RS24690 0.87 -3.4 5,531,603 + PFLU_RS24690 0.87 -1.0 5,531,604 - PFLU_RS24690 0.87 +2.2 5,531,604 - PFLU_RS24690 0.87 -3.1 5,531,604 - PFLU_RS24690 0.87 -1.7 5,531,624 + PFLU_RS24690 0.88 +0.9 5,531,624 + PFLU_RS24690 0.88 +1.0 5,531,625 - PFLU_RS24690 0.88 -2.2 5,531,672 + -0.6 5,531,672 + -1.7 5,531,672 + +1.2 5,531,672 + -2.2 5,531,672 + +0.5 5,531,673 - -0.8 5,531,675 + +2.0 5,531,675 + -0.3 5,531,675 + -2.7 5,531,675 + -2.6 5,531,675 + -0.0 5,531,675 + -3.4 5,531,676 - -2.6 5,531,676 - -1.9 5,531,676 - -1.0 5,531,764 + +1.4 5,531,764 + +1.6 5,531,764 + -0.7 5,531,765 - -3.4 5,531,779 + -1.6 5,531,780 - +1.3 5,531,780 - +0.5 5,531,792 + +0.5 5,531,792 + +1.0 5,531,792 + +0.3 5,531,792 + +2.0 5,531,792 + +0.4 5,531,792 + -0.6 5,531,792 + -1.4 5,531,792 + +0.0 5,531,792 + +0.7 5,531,792 + -2.2 5,531,793 - -0.5 5,531,793 - -2.3 5,531,793 - +0.5 5,531,793 - +0.1 5,531,793 - -0.6 5,531,859 + -3.6 5,531,860 - +1.6 5,531,860 - -1.2 5,531,860 - -0.0 5,531,882 + -0.1 5,531,882 + -0.8 5,531,883 - -0.2 5,532,010 + PFLU_RS24695 0.54 +1.0 5,532,123 - +1.3 5,532,123 - -2.0 5,532,182 + +2.7 5,532,183 - +0.3 5,532,183 - -1.4 5,532,509 + PFLU_RS24700 0.15 +0.2 5,532,509 + PFLU_RS24700 0.15 -1.7
Or see this region's nucleotide sequence