Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS04160 and PFLU_RS04165 are separated by 78 nucleotides PFLU_RS04165 and PFLU_RS04170 are separated by 144 nucleotides
PFLU_RS04160: PFLU_RS04160 - tryptophan--tRNA ligase, at 943,684 to 945,039
_RS04160
PFLU_RS04165: PFLU_RS04165 - cell division protein ZapE, at 945,118 to 946,212
_RS04165
PFLU_RS04170: PFLU_RS04170 - GlxA family transcriptional regulator, at 946,357 to 947,352
_RS04170
Position (kb) 945
946
947 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 945.079 kb on + strand at 945.079 kb on + strand at 945.117 kb on + strand at 945.117 kb on + strand at 945.118 kb on - strand at 945.118 kb on - strand at 945.118 kb on - strand at 945.118 kb on - strand at 945.128 kb on + strand at 945.129 kb on - strand at 945.129 kb on - strand at 945.136 kb on + strand at 945.137 kb on - strand at 945.137 kb on - strand at 945.304 kb on + strand, within PFLU_RS04165 at 945.304 kb on + strand, within PFLU_RS04165 at 945.304 kb on + strand, within PFLU_RS04165 at 945.304 kb on + strand, within PFLU_RS04165 at 945.304 kb on + strand, within PFLU_RS04165 at 945.304 kb on + strand, within PFLU_RS04165 at 945.304 kb on + strand, within PFLU_RS04165 at 945.304 kb on + strand, within PFLU_RS04165 at 945.304 kb on + strand, within PFLU_RS04165 at 945.304 kb on + strand, within PFLU_RS04165 at 945.304 kb on + strand, within PFLU_RS04165 at 945.305 kb on - strand, within PFLU_RS04165 at 945.305 kb on - strand, within PFLU_RS04165 at 945.337 kb on + strand, within PFLU_RS04165 at 945.337 kb on + strand, within PFLU_RS04165 at 945.337 kb on + strand, within PFLU_RS04165 at 945.337 kb on + strand, within PFLU_RS04165 at 945.337 kb on + strand, within PFLU_RS04165 at 945.338 kb on - strand, within PFLU_RS04165 at 945.338 kb on - strand, within PFLU_RS04165 at 945.338 kb on - strand, within PFLU_RS04165 at 945.338 kb on - strand, within PFLU_RS04165 at 945.338 kb on - strand, within PFLU_RS04165 at 945.625 kb on + strand, within PFLU_RS04165 at 945.625 kb on + strand, within PFLU_RS04165 at 945.625 kb on + strand, within PFLU_RS04165 at 945.625 kb on + strand, within PFLU_RS04165 at 945.625 kb on + strand, within PFLU_RS04165 at 945.625 kb on + strand, within PFLU_RS04165 at 945.626 kb on - strand, within PFLU_RS04165 at 945.626 kb on - strand, within PFLU_RS04165 at 945.626 kb on - strand, within PFLU_RS04165 at 945.626 kb on - strand, within PFLU_RS04165 at 945.626 kb on - strand, within PFLU_RS04165 at 945.626 kb on - strand, within PFLU_RS04165 at 945.626 kb on - strand, within PFLU_RS04165 at 945.626 kb on - strand, within PFLU_RS04165 at 945.949 kb on + strand, within PFLU_RS04165 at 945.949 kb on + strand, within PFLU_RS04165 at 945.949 kb on + strand, within PFLU_RS04165 at 945.949 kb on + strand, within PFLU_RS04165 at 945.950 kb on - strand, within PFLU_RS04165 at 945.950 kb on - strand, within PFLU_RS04165 at 945.950 kb on - strand, within PFLU_RS04165 at 945.950 kb on - strand, within PFLU_RS04165 at 945.950 kb on - strand, within PFLU_RS04165 at 945.950 kb on - strand, within PFLU_RS04165 at 945.950 kb on - strand, within PFLU_RS04165 at 946.060 kb on + strand, within PFLU_RS04165 at 946.060 kb on + strand, within PFLU_RS04165 at 946.060 kb on + strand, within PFLU_RS04165 at 946.061 kb on - strand, within PFLU_RS04165 at 946.061 kb on - strand, within PFLU_RS04165 at 946.061 kb on - strand, within PFLU_RS04165 at 946.061 kb on - strand, within PFLU_RS04165 at 946.061 kb on - strand, within PFLU_RS04165 at 946.069 kb on - strand, within PFLU_RS04165 at 946.069 kb on - strand, within PFLU_RS04165 at 946.115 kb on - strand at 946.115 kb on - strand at 946.115 kb on - strand at 946.115 kb on - strand at 946.115 kb on - strand at 946.115 kb on - strand at 946.115 kb on - strand at 946.210 kb on + strand at 946.210 kb on + strand at 946.210 kb on + strand at 946.210 kb on + strand at 946.211 kb on - strand at 946.353 kb on + strand at 946.353 kb on + strand at 946.353 kb on + strand at 946.353 kb on + strand at 946.354 kb on - strand at 946.354 kb on - strand at 946.354 kb on - strand at 946.354 kb on - strand at 946.354 kb on - strand at 946.448 kb on + strand at 946.460 kb on + strand, within PFLU_RS04170 at 946.460 kb on + strand, within PFLU_RS04170 at 946.460 kb on + strand, within PFLU_RS04170 at 946.460 kb on + strand, within PFLU_RS04170 at 946.460 kb on + strand, within PFLU_RS04170 at 946.460 kb on + strand, within PFLU_RS04170 at 946.460 kb on + strand, within PFLU_RS04170 at 946.460 kb on + strand, within PFLU_RS04170 at 946.460 kb on + strand, within PFLU_RS04170 at 946.460 kb on + strand, within PFLU_RS04170 at 946.461 kb on - strand, within PFLU_RS04170 at 946.461 kb on - strand, within PFLU_RS04170 at 946.461 kb on - strand, within PFLU_RS04170 at 946.461 kb on - strand, within PFLU_RS04170 at 946.461 kb on - strand, within PFLU_RS04170 at 946.461 kb on - strand, within PFLU_RS04170 at 946.461 kb on - strand, within PFLU_RS04170 at 946.461 kb on - strand, within PFLU_RS04170 at 946.461 kb on - strand, within PFLU_RS04170 at 946.461 kb on - strand, within PFLU_RS04170 at 946.461 kb on - strand, within PFLU_RS04170 at 946.535 kb on + strand, within PFLU_RS04170 at 946.535 kb on + strand, within PFLU_RS04170 at 946.535 kb on + strand, within PFLU_RS04170 at 946.535 kb on + strand, within PFLU_RS04170 at 946.535 kb on + strand, within PFLU_RS04170 at 946.535 kb on + strand, within PFLU_RS04170 at 946.535 kb on + strand, within PFLU_RS04170 at 946.535 kb on + strand, within PFLU_RS04170 at 946.536 kb on - strand, within PFLU_RS04170 at 946.536 kb on - strand, within PFLU_RS04170 at 946.536 kb on - strand, within PFLU_RS04170 at 946.536 kb on - strand, within PFLU_RS04170 at 946.536 kb on - strand, within PFLU_RS04170 at 946.536 kb on - strand, within PFLU_RS04170 at 946.548 kb on + strand, within PFLU_RS04170 at 946.548 kb on + strand, within PFLU_RS04170 at 946.548 kb on + strand, within PFLU_RS04170 at 946.548 kb on + strand, within PFLU_RS04170 at 946.549 kb on - strand, within PFLU_RS04170 at 946.549 kb on - strand, within PFLU_RS04170 at 946.549 kb on - strand, within PFLU_RS04170 at 946.549 kb on - strand, within PFLU_RS04170 at 946.549 kb on - strand, within PFLU_RS04170 at 946.549 kb on - strand, within PFLU_RS04170 at 946.549 kb on - strand, within PFLU_RS04170 at 946.549 kb on - strand, within PFLU_RS04170 at 946.727 kb on + strand, within PFLU_RS04170 at 946.727 kb on + strand, within PFLU_RS04170 at 946.727 kb on + strand, within PFLU_RS04170 at 946.727 kb on + strand, within PFLU_RS04170 at 946.727 kb on + strand, within PFLU_RS04170 at 946.727 kb on + strand, within PFLU_RS04170 at 946.728 kb on - strand, within PFLU_RS04170 at 946.728 kb on - strand, within PFLU_RS04170 at 946.728 kb on - strand, within PFLU_RS04170 at 946.728 kb on - strand, within PFLU_RS04170 at 946.728 kb on - strand, within PFLU_RS04170 at 946.795 kb on + strand, within PFLU_RS04170 at 946.805 kb on + strand, within PFLU_RS04170 at 946.805 kb on + strand, within PFLU_RS04170 at 946.805 kb on + strand, within PFLU_RS04170 at 946.805 kb on + strand, within PFLU_RS04170 at 946.805 kb on + strand, within PFLU_RS04170 at 946.806 kb on - strand, within PFLU_RS04170 at 946.806 kb on - strand, within PFLU_RS04170 at 946.806 kb on - strand, within PFLU_RS04170 at 946.806 kb on - strand, within PFLU_RS04170 at 946.806 kb on - strand, within PFLU_RS04170 at 946.806 kb on - strand, within PFLU_RS04170 at 946.806 kb on - strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.811 kb on + strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.812 kb on - strand, within PFLU_RS04170 at 946.847 kb on + strand, within PFLU_RS04170 at 946.847 kb on + strand, within PFLU_RS04170 at 946.847 kb on + strand, within PFLU_RS04170 at 946.848 kb on - strand, within PFLU_RS04170 at 946.848 kb on - strand, within PFLU_RS04170 at 946.848 kb on - strand, within PFLU_RS04170 at 946.934 kb on + strand, within PFLU_RS04170 at 946.934 kb on + strand, within PFLU_RS04170 at 946.934 kb on + strand, within PFLU_RS04170 at 946.934 kb on + strand, within PFLU_RS04170 at 946.934 kb on + strand, within PFLU_RS04170 at 946.934 kb on + strand, within PFLU_RS04170 at 946.934 kb on + strand, within PFLU_RS04170 at 946.935 kb on - strand, within PFLU_RS04170 at 946.935 kb on - strand, within PFLU_RS04170 at 947.005 kb on + strand, within PFLU_RS04170 at 947.006 kb on - strand, within PFLU_RS04170 at 947.006 kb on - strand, within PFLU_RS04170
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs remove 945,079 + -1.1 945,079 + -3.4 945,117 + -1.2 945,117 + +0.6 945,118 - -2.3 945,118 - -1.6 945,118 - -1.5 945,118 - -0.5 945,128 + -3.3 945,129 - -2.0 945,129 - +0.1 945,136 + -1.3 945,137 - +2.7 945,137 - +0.7 945,304 + PFLU_RS04165 0.17 -0.0 945,304 + PFLU_RS04165 0.17 -0.3 945,304 + PFLU_RS04165 0.17 +1.1 945,304 + PFLU_RS04165 0.17 +1.7 945,304 + PFLU_RS04165 0.17 -0.6 945,304 + PFLU_RS04165 0.17 -1.4 945,304 + PFLU_RS04165 0.17 -2.4 945,304 + PFLU_RS04165 0.17 -1.4 945,304 + PFLU_RS04165 0.17 -1.2 945,304 + PFLU_RS04165 0.17 +0.1 945,304 + PFLU_RS04165 0.17 +1.7 945,305 - PFLU_RS04165 0.17 -0.3 945,305 - PFLU_RS04165 0.17 -2.2 945,337 + PFLU_RS04165 0.20 -0.4 945,337 + PFLU_RS04165 0.20 -0.3 945,337 + PFLU_RS04165 0.20 -0.6 945,337 + PFLU_RS04165 0.20 +0.3 945,337 + PFLU_RS04165 0.20 -1.1 945,338 - PFLU_RS04165 0.20 +1.7 945,338 - PFLU_RS04165 0.20 -1.6 945,338 - PFLU_RS04165 0.20 +0.2 945,338 - PFLU_RS04165 0.20 -1.1 945,338 - PFLU_RS04165 0.20 -1.7 945,625 + PFLU_RS04165 0.46 -1.3 945,625 + PFLU_RS04165 0.46 -1.4 945,625 + PFLU_RS04165 0.46 -0.3 945,625 + PFLU_RS04165 0.46 +1.4 945,625 + PFLU_RS04165 0.46 -1.1 945,625 + PFLU_RS04165 0.46 +2.2 945,626 - PFLU_RS04165 0.46 +3.1 945,626 - PFLU_RS04165 0.46 +0.7 945,626 - PFLU_RS04165 0.46 -1.1 945,626 - PFLU_RS04165 0.46 -3.2 945,626 - PFLU_RS04165 0.46 +0.7 945,626 - PFLU_RS04165 0.46 -0.3 945,626 - PFLU_RS04165 0.46 +0.4 945,626 - PFLU_RS04165 0.46 -1.4 945,949 + PFLU_RS04165 0.76 +0.1 945,949 + PFLU_RS04165 0.76 +0.1 945,949 + PFLU_RS04165 0.76 +0.6 945,949 + PFLU_RS04165 0.76 +0.7 945,950 - PFLU_RS04165 0.76 -0.6 945,950 - PFLU_RS04165 0.76 -0.1 945,950 - PFLU_RS04165 0.76 -1.4 945,950 - PFLU_RS04165 0.76 -2.3 945,950 - PFLU_RS04165 0.76 -0.9 945,950 - PFLU_RS04165 0.76 -0.9 945,950 - PFLU_RS04165 0.76 -1.3 946,060 + PFLU_RS04165 0.86 +1.3 946,060 + PFLU_RS04165 0.86 -2.2 946,060 + PFLU_RS04165 0.86 -0.2 946,061 - PFLU_RS04165 0.86 -1.3 946,061 - PFLU_RS04165 0.86 -0.4 946,061 - PFLU_RS04165 0.86 -1.1 946,061 - PFLU_RS04165 0.86 -0.9 946,061 - PFLU_RS04165 0.86 -0.3 946,069 - PFLU_RS04165 0.87 -0.3 946,069 - PFLU_RS04165 0.87 -1.6 946,115 - -1.4 946,115 - -2.0 946,115 - +0.1 946,115 - -0.1 946,115 - -0.0 946,115 - +1.7 946,115 - -0.6 946,210 + -2.1 946,210 + -2.6 946,210 + -3.8 946,210 + -0.0 946,211 - -0.6 946,353 + -2.6 946,353 + -1.4 946,353 + +1.1 946,353 + +0.2 946,354 - -1.4 946,354 - -3.2 946,354 - +1.2 946,354 - -2.2 946,354 - -0.2 946,448 + +0.0 946,460 + PFLU_RS04170 0.10 +2.6 946,460 + PFLU_RS04170 0.10 +1.2 946,460 + PFLU_RS04170 0.10 -1.7 946,460 + PFLU_RS04170 0.10 -0.6 946,460 + PFLU_RS04170 0.10 +0.7 946,460 + PFLU_RS04170 0.10 +0.1 946,460 + PFLU_RS04170 0.10 -1.4 946,460 + PFLU_RS04170 0.10 -3.1 946,460 + PFLU_RS04170 0.10 -2.7 946,460 + PFLU_RS04170 0.10 +0.4 946,461 - PFLU_RS04170 0.10 -2.5 946,461 - PFLU_RS04170 0.10 +0.4 946,461 - PFLU_RS04170 0.10 -1.9 946,461 - PFLU_RS04170 0.10 -3.3 946,461 - PFLU_RS04170 0.10 -0.2 946,461 - PFLU_RS04170 0.10 -0.2 946,461 - PFLU_RS04170 0.10 +0.5 946,461 - PFLU_RS04170 0.10 -2.0 946,461 - PFLU_RS04170 0.10 +0.1 946,461 - PFLU_RS04170 0.10 +0.7 946,461 - PFLU_RS04170 0.10 -4.0 946,535 + PFLU_RS04170 0.18 -0.9 946,535 + PFLU_RS04170 0.18 -0.1 946,535 + PFLU_RS04170 0.18 -3.8 946,535 + PFLU_RS04170 0.18 -0.7 946,535 + PFLU_RS04170 0.18 -3.7 946,535 + PFLU_RS04170 0.18 -1.3 946,535 + PFLU_RS04170 0.18 -0.1 946,535 + PFLU_RS04170 0.18 -0.1 946,536 - PFLU_RS04170 0.18 -0.8 946,536 - PFLU_RS04170 0.18 +0.2 946,536 - PFLU_RS04170 0.18 -1.2 946,536 - PFLU_RS04170 0.18 -1.4 946,536 - PFLU_RS04170 0.18 +1.5 946,536 - PFLU_RS04170 0.18 -3.0 946,548 + PFLU_RS04170 0.19 +1.1 946,548 + PFLU_RS04170 0.19 +2.2 946,548 + PFLU_RS04170 0.19 -2.2 946,548 + PFLU_RS04170 0.19 -0.4 946,549 - PFLU_RS04170 0.19 +0.3 946,549 - PFLU_RS04170 0.19 -0.7 946,549 - PFLU_RS04170 0.19 -2.3 946,549 - PFLU_RS04170 0.19 +0.1 946,549 - PFLU_RS04170 0.19 +1.7 946,549 - PFLU_RS04170 0.19 +0.9 946,549 - PFLU_RS04170 0.19 -0.4 946,549 - PFLU_RS04170 0.19 -0.5 946,727 + PFLU_RS04170 0.37 -0.6 946,727 + PFLU_RS04170 0.37 +0.8 946,727 + PFLU_RS04170 0.37 -2.8 946,727 + PFLU_RS04170 0.37 +0.9 946,727 + PFLU_RS04170 0.37 -0.1 946,727 + PFLU_RS04170 0.37 +0.0 946,728 - PFLU_RS04170 0.37 +1.5 946,728 - PFLU_RS04170 0.37 +0.7 946,728 - PFLU_RS04170 0.37 -1.7 946,728 - PFLU_RS04170 0.37 -1.7 946,728 - PFLU_RS04170 0.37 +1.7 946,795 + PFLU_RS04170 0.44 +0.8 946,805 + PFLU_RS04170 0.45 +0.5 946,805 + PFLU_RS04170 0.45 -1.7 946,805 + PFLU_RS04170 0.45 -1.3 946,805 + PFLU_RS04170 0.45 -0.6 946,805 + PFLU_RS04170 0.45 +0.2 946,806 - PFLU_RS04170 0.45 -3.0 946,806 - PFLU_RS04170 0.45 +0.5 946,806 - PFLU_RS04170 0.45 +0.5 946,806 - PFLU_RS04170 0.45 +2.1 946,806 - PFLU_RS04170 0.45 -0.1 946,806 - PFLU_RS04170 0.45 -2.0 946,806 - PFLU_RS04170 0.45 -4.0 946,811 + PFLU_RS04170 0.46 +0.6 946,811 + PFLU_RS04170 0.46 +2.3 946,811 + PFLU_RS04170 0.46 +1.7 946,811 + PFLU_RS04170 0.46 -2.5 946,811 + PFLU_RS04170 0.46 +0.3 946,811 + PFLU_RS04170 0.46 +1.1 946,811 + PFLU_RS04170 0.46 -0.5 946,811 + PFLU_RS04170 0.46 +0.7 946,811 + PFLU_RS04170 0.46 +0.6 946,811 + PFLU_RS04170 0.46 -3.2 946,811 + PFLU_RS04170 0.46 -3.4 946,811 + PFLU_RS04170 0.46 -2.0 946,811 + PFLU_RS04170 0.46 -0.3 946,811 + PFLU_RS04170 0.46 -0.6 946,811 + PFLU_RS04170 0.46 -0.2 946,811 + PFLU_RS04170 0.46 +0.9 946,811 + PFLU_RS04170 0.46 -2.4 946,811 + PFLU_RS04170 0.46 -0.7 946,811 + PFLU_RS04170 0.46 -0.9 946,811 + PFLU_RS04170 0.46 -0.8 946,812 - PFLU_RS04170 0.46 -1.3 946,812 - PFLU_RS04170 0.46 -0.3 946,812 - PFLU_RS04170 0.46 +0.7 946,812 - PFLU_RS04170 0.46 -2.7 946,812 - PFLU_RS04170 0.46 -0.5 946,812 - PFLU_RS04170 0.46 +1.5 946,812 - PFLU_RS04170 0.46 +1.4 946,812 - PFLU_RS04170 0.46 +1.9 946,812 - PFLU_RS04170 0.46 +1.0 946,812 - PFLU_RS04170 0.46 -2.2 946,812 - PFLU_RS04170 0.46 +0.4 946,812 - PFLU_RS04170 0.46 -4.0 946,812 - PFLU_RS04170 0.46 +1.7 946,812 - PFLU_RS04170 0.46 -0.1 946,812 - PFLU_RS04170 0.46 +0.5 946,812 - PFLU_RS04170 0.46 -0.6 946,847 + PFLU_RS04170 0.49 -0.6 946,847 + PFLU_RS04170 0.49 -0.4 946,847 + PFLU_RS04170 0.49 +0.3 946,848 - PFLU_RS04170 0.49 -0.3 946,848 - PFLU_RS04170 0.49 -1.4 946,848 - PFLU_RS04170 0.49 +0.4 946,934 + PFLU_RS04170 0.58 -2.6 946,934 + PFLU_RS04170 0.58 -2.1 946,934 + PFLU_RS04170 0.58 -1.7 946,934 + PFLU_RS04170 0.58 +0.0 946,934 + PFLU_RS04170 0.58 -0.9 946,934 + PFLU_RS04170 0.58 -1.6 946,934 + PFLU_RS04170 0.58 -0.3 946,935 - PFLU_RS04170 0.58 +1.4 946,935 - PFLU_RS04170 0.58 -2.4 947,005 + PFLU_RS04170 0.65 -0.1 947,006 - PFLU_RS04170 0.65 -3.8 947,006 - PFLU_RS04170 0.65 +0.3
Or see this region's nucleotide sequence