Experiment: BHIS with Zinc Pyrithione 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2038 and BT2039 are separated by 129 nucleotides BT2039 and BT2040 are separated by 49 nucleotides
BT2038: BT2038 - cation efflux system protein (NCBI ptt file), at 2,557,389 to 2,558,660
BT2038
BT2039: BT2039 - cation efflux system protein, AcrB/AcrD/AcrF family protein (NCBI ptt file), at 2,558,790 to 2,561,918
BT2039
BT2040: BT2040 - hypothetical protein (NCBI ptt file), at 2,561,968 to 2,563,161
BT2040
Position (kb)
2558
2559
2560
2561
2562 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2557.860 kb on - strand, within BT2038 at 2557.895 kb on - strand, within BT2038 at 2557.915 kb on - strand, within BT2038 at 2557.915 kb on - strand, within BT2038 at 2557.936 kb on - strand, within BT2038 at 2557.936 kb on - strand, within BT2038 at 2558.124 kb on + strand, within BT2038 at 2558.128 kb on + strand, within BT2038 at 2558.251 kb on - strand, within BT2038 at 2558.256 kb on - strand, within BT2038 at 2558.479 kb on - strand, within BT2038 at 2558.807 kb on - strand at 2558.807 kb on - strand at 2558.862 kb on - strand at 2558.874 kb on + strand at 2558.875 kb on - strand at 2558.875 kb on - strand at 2558.897 kb on - strand at 2559.095 kb on + strand at 2559.095 kb on + strand at 2559.096 kb on - strand at 2559.096 kb on - strand at 2559.097 kb on + strand at 2559.097 kb on + strand at 2559.098 kb on - strand at 2559.099 kb on + strand at 2559.100 kb on - strand at 2559.100 kb on - strand at 2559.471 kb on + strand, within BT2039 at 2559.472 kb on - strand, within BT2039 at 2559.474 kb on - strand, within BT2039 at 2559.584 kb on - strand, within BT2039 at 2559.971 kb on + strand, within BT2039 at 2560.030 kb on - strand, within BT2039 at 2560.442 kb on - strand, within BT2039 at 2560.522 kb on + strand, within BT2039 at 2560.523 kb on - strand, within BT2039 at 2560.523 kb on - strand, within BT2039 at 2560.645 kb on + strand, within BT2039 at 2560.800 kb on - strand, within BT2039 at 2560.812 kb on - strand, within BT2039 at 2561.008 kb on - strand, within BT2039 at 2561.537 kb on - strand, within BT2039 at 2561.648 kb on - strand at 2561.649 kb on + strand at 2561.807 kb on - strand at 2561.916 kb on + strand at 2561.917 kb on - strand at 2561.917 kb on - strand at 2561.938 kb on - strand at 2561.940 kb on + strand at 2561.941 kb on - strand at 2561.941 kb on - strand at 2561.944 kb on + strand at 2562.168 kb on - strand, within BT2040 at 2562.172 kb on + strand, within BT2040 at 2562.257 kb on - strand, within BT2040 at 2562.257 kb on - strand at 2562.257 kb on - strand, within BT2040 at 2562.257 kb on - strand, within BT2040 at 2562.366 kb on + strand, within BT2040 at 2562.549 kb on + strand, within BT2040 at 2562.550 kb on - strand, within BT2040 at 2562.573 kb on - strand, within BT2040 at 2562.807 kb on - strand, within BT2040
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Zinc Pyrithione 0.05 mM remove 2,557,860 - BT2038 0.37 -1.7 2,557,895 - BT2038 0.40 -2.2 2,557,915 - BT2038 0.41 -0.8 2,557,915 - BT2038 0.41 -2.4 2,557,936 - BT2038 0.43 -2.2 2,557,936 - BT2038 0.43 -2.2 2,558,124 + BT2038 0.58 -1.9 2,558,128 + BT2038 0.58 -1.4 2,558,251 - BT2038 0.68 -2.6 2,558,256 - BT2038 0.68 -0.6 2,558,479 - BT2038 0.86 -0.6 2,558,807 - +1.0 2,558,807 - +0.0 2,558,862 - +1.0 2,558,874 + -2.9 2,558,875 - -3.1 2,558,875 - -1.2 2,558,897 - +0.0 2,559,095 + +1.0 2,559,095 + -2.7 2,559,096 - -2.2 2,559,096 - -0.6 2,559,097 + +0.3 2,559,097 + -3.0 2,559,098 - -2.3 2,559,099 + -0.9 2,559,100 - -1.8 2,559,100 - -1.3 2,559,471 + BT2039 0.22 -1.9 2,559,472 - BT2039 0.22 -1.9 2,559,474 - BT2039 0.22 -1.3 2,559,584 - BT2039 0.25 -1.6 2,559,971 + BT2039 0.38 -0.8 2,560,030 - BT2039 0.40 -1.5 2,560,442 - BT2039 0.53 -3.0 2,560,522 + BT2039 0.55 -2.8 2,560,523 - BT2039 0.55 -0.6 2,560,523 - BT2039 0.55 -1.6 2,560,645 + BT2039 0.59 +0.0 2,560,800 - BT2039 0.64 -1.0 2,560,812 - BT2039 0.65 +1.0 2,561,008 - BT2039 0.71 -3.7 2,561,537 - BT2039 0.88 -1.6 2,561,648 - -1.0 2,561,649 + -0.6 2,561,807 - -0.6 2,561,916 + -1.3 2,561,917 - -2.7 2,561,917 - -1.8 2,561,938 - -0.6 2,561,940 + -1.6 2,561,941 - +0.0 2,561,941 - +0.0 2,561,944 + -1.3 2,562,168 - BT2040 0.17 -3.0 2,562,172 + BT2040 0.17 -1.8 2,562,257 - BT2040 0.24 +2.0 2,562,257 - +0.0 2,562,257 - BT2040 0.24 -1.0 2,562,257 - BT2040 0.24 -2.4 2,562,366 + BT2040 0.33 -2.3 2,562,549 + BT2040 0.49 -3.6 2,562,550 - BT2040 0.49 -2.7 2,562,573 - BT2040 0.51 -0.8 2,562,807 - BT2040 0.70 -0.6
Or see this region's nucleotide sequence