Experiment: MoLS4 without vitamins with Casaminos 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0622 and nrfH are separated by 342 nucleotides nrfH and nrfA overlap by 8 nucleotides nrfA and ilvN-1 are separated by 432 nucleotides ilvN-1 and ptB are separated by 3 nucleotides
DVU0622: DVU0622 - sensor histidine kinase/response regulator (TIGR), at 688,231 to 690,729
DVU0622
DVU0624: nrfH - NapC/NirT cytochrome c family protein (TIGR), at 691,072 to 691,551
nrfH
DVU0625: nrfA - cytochrome c nitrite reductase, catalytic subunit NrfA, putative (Shelley Haveman), at 691,544 to 693,118
nrfA
DVU0626: ilvN-1 - acetolactate synthase, small subunit (TIGR), at 693,551 to 694,030
ilvN-1
DVU0627: ptB - phosphotransbutyrylase (TIGR), at 694,034 to 695,065
ptB
Position (kb) 691
692
693
694 Strain fitness (log2 ratio) -2
-1
0
1
2 at 690.565 kb on + strand at 690.579 kb on + strand at 690.614 kb on - strand at 690.652 kb on + strand at 690.652 kb on + strand at 690.660 kb on - strand at 690.674 kb on + strand at 690.679 kb on + strand at 690.714 kb on + strand at 690.722 kb on - strand at 690.722 kb on - strand at 690.722 kb on - strand at 690.727 kb on - strand at 690.966 kb on - strand at 691.029 kb on + strand at 691.213 kb on - strand, within nrfH at 691.221 kb on - strand, within nrfH at 691.311 kb on + strand, within nrfH at 691.337 kb on - strand, within nrfH at 691.402 kb on + strand, within nrfH at 691.415 kb on + strand, within nrfH at 691.424 kb on - strand, within nrfH at 691.424 kb on - strand, within nrfH at 691.431 kb on + strand, within nrfH at 691.465 kb on + strand, within nrfH at 691.481 kb on - strand, within nrfH at 691.498 kb on + strand, within nrfH at 691.498 kb on + strand, within nrfH at 691.503 kb on + strand, within nrfH at 691.506 kb on - strand at 691.569 kb on - strand at 691.774 kb on - strand, within nrfA at 691.779 kb on - strand, within nrfA at 691.823 kb on + strand, within nrfA at 691.855 kb on + strand, within nrfA at 691.863 kb on - strand, within nrfA at 691.908 kb on - strand, within nrfA at 691.990 kb on + strand, within nrfA at 691.990 kb on + strand, within nrfA at 692.016 kb on + strand, within nrfA at 692.018 kb on + strand, within nrfA at 692.120 kb on + strand, within nrfA at 692.120 kb on + strand, within nrfA at 692.127 kb on + strand, within nrfA at 692.127 kb on + strand, within nrfA at 692.135 kb on - strand, within nrfA at 692.135 kb on - strand, within nrfA at 692.137 kb on - strand, within nrfA at 692.187 kb on - strand, within nrfA at 692.230 kb on - strand, within nrfA at 692.246 kb on + strand, within nrfA at 692.335 kb on + strand, within nrfA at 692.402 kb on - strand, within nrfA at 692.455 kb on + strand, within nrfA at 692.515 kb on - strand, within nrfA at 692.658 kb on + strand, within nrfA at 692.658 kb on + strand, within nrfA at 692.708 kb on + strand, within nrfA at 692.770 kb on - strand, within nrfA at 692.776 kb on - strand, within nrfA at 692.959 kb on + strand, within nrfA at 693.037 kb on - strand at 693.061 kb on + strand at 693.061 kb on + strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.076 kb on + strand at 693.093 kb on + strand at 693.095 kb on + strand at 693.103 kb on - strand at 693.140 kb on + strand at 693.148 kb on - strand at 693.185 kb on + strand at 693.295 kb on + strand at 693.301 kb on - strand at 693.312 kb on + strand at 693.330 kb on - strand at 693.332 kb on + strand at 693.353 kb on + strand at 693.479 kb on + strand at 693.506 kb on - strand at 693.513 kb on + strand at 693.521 kb on - strand at 693.530 kb on + strand at 693.616 kb on - strand, within ilvN-1 at 693.628 kb on - strand, within ilvN-1 at 693.735 kb on - strand, within ilvN-1 at 693.814 kb on + strand, within ilvN-1 at 693.814 kb on + strand, within ilvN-1 at 693.814 kb on + strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.904 kb on + strand, within ilvN-1 at 693.947 kb on + strand, within ilvN-1 at 693.984 kb on + strand at 693.984 kb on + strand at 694.051 kb on - strand at 694.085 kb on - strand at 694.113 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.05% remove 690,565 + -1.1 690,579 + -0.5 690,614 - +0.9 690,652 + -2.1 690,652 + -0.0 690,660 - -1.5 690,674 + +0.7 690,679 + -0.2 690,714 + -0.1 690,722 - -0.0 690,722 - -2.1 690,722 - -1.4 690,727 - -1.2 690,966 - -0.3 691,029 + +0.2 691,213 - nrfH DVU0624 0.29 -0.1 691,221 - nrfH DVU0624 0.31 -0.2 691,311 + nrfH DVU0624 0.50 -1.0 691,337 - nrfH DVU0624 0.55 +0.1 691,402 + nrfH DVU0624 0.69 +0.1 691,415 + nrfH DVU0624 0.71 +0.9 691,424 - nrfH DVU0624 0.73 +0.6 691,424 - nrfH DVU0624 0.73 -0.2 691,431 + nrfH DVU0624 0.75 +0.3 691,465 + nrfH DVU0624 0.82 +0.9 691,481 - nrfH DVU0624 0.85 +0.0 691,498 + nrfH DVU0624 0.89 +0.2 691,498 + nrfH DVU0624 0.89 -1.2 691,503 + nrfH DVU0624 0.90 +1.5 691,506 - +0.7 691,569 - +2.5 691,774 - nrfA DVU0625 0.15 -0.2 691,779 - nrfA DVU0625 0.15 +0.3 691,823 + nrfA DVU0625 0.18 +0.6 691,855 + nrfA DVU0625 0.20 -0.1 691,863 - nrfA DVU0625 0.20 -1.0 691,908 - nrfA DVU0625 0.23 -0.1 691,990 + nrfA DVU0625 0.28 +0.2 691,990 + nrfA DVU0625 0.28 -0.9 692,016 + nrfA DVU0625 0.30 -0.1 692,018 + nrfA DVU0625 0.30 +0.1 692,120 + nrfA DVU0625 0.37 +0.0 692,120 + nrfA DVU0625 0.37 -0.6 692,127 + nrfA DVU0625 0.37 +0.1 692,127 + nrfA DVU0625 0.37 -0.3 692,135 - nrfA DVU0625 0.38 +0.5 692,135 - nrfA DVU0625 0.38 -0.5 692,137 - nrfA DVU0625 0.38 -0.4 692,187 - nrfA DVU0625 0.41 -0.3 692,230 - nrfA DVU0625 0.44 +1.3 692,246 + nrfA DVU0625 0.45 -0.1 692,335 + nrfA DVU0625 0.50 -0.3 692,402 - nrfA DVU0625 0.54 -0.4 692,455 + nrfA DVU0625 0.58 -0.1 692,515 - nrfA DVU0625 0.62 +0.5 692,658 + nrfA DVU0625 0.71 -0.3 692,658 + nrfA DVU0625 0.71 +0.3 692,708 + nrfA DVU0625 0.74 -0.1 692,770 - nrfA DVU0625 0.78 -0.7 692,776 - nrfA DVU0625 0.78 -0.3 692,959 + nrfA DVU0625 0.90 -0.4 693,037 - +0.1 693,061 + -0.4 693,061 + -0.9 693,069 - -0.3 693,069 - -0.4 693,069 - -1.5 693,069 - -0.2 693,069 - +0.1 693,069 - -1.0 693,069 - -0.1 693,076 + -0.1 693,093 + -0.1 693,095 + -0.3 693,103 - -0.1 693,140 + -0.2 693,148 - +0.3 693,185 + -0.1 693,295 + +0.1 693,301 - -1.1 693,312 + -0.3 693,330 - -0.5 693,332 + -2.5 693,353 + -1.7 693,479 + -0.5 693,506 - -0.5 693,513 + -0.4 693,521 - -0.6 693,530 + +1.9 693,616 - ilvN-1 DVU0626 0.14 -0.3 693,628 - ilvN-1 DVU0626 0.16 +0.2 693,735 - ilvN-1 DVU0626 0.38 -0.1 693,814 + ilvN-1 DVU0626 0.55 +1.3 693,814 + ilvN-1 DVU0626 0.55 -0.3 693,814 + ilvN-1 DVU0626 0.55 -0.3 693,822 - ilvN-1 DVU0626 0.56 -0.0 693,822 - ilvN-1 DVU0626 0.56 -0.7 693,822 - ilvN-1 DVU0626 0.56 -1.4 693,822 - ilvN-1 DVU0626 0.56 -0.2 693,822 - ilvN-1 DVU0626 0.56 -0.1 693,822 - ilvN-1 DVU0626 0.56 -0.2 693,822 - ilvN-1 DVU0626 0.56 -0.1 693,904 + ilvN-1 DVU0626 0.74 -0.1 693,947 + ilvN-1 DVU0626 0.82 -0.0 693,984 + -0.8 693,984 + -0.3 694,051 - -0.1 694,085 - -0.3 694,113 + -1.1
Or see this region's nucleotide sequence