Experiment: MoLS4 without vitamins with Casaminos 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrS and tRNA-Val are separated by 85 nucleotides tRNA-Val and DVU2539 are separated by 24 nucleotides DVU2539 and DVU2540 are separated by 5 nucleotides DVU2540 and DVU2541 are separated by 7 nucleotides
DVU2538: thrS - threonyl-tRNA synthetase (TIGR), at 2,649,245 to 2,651,179
thrS
DVU_tRNA-Val-2: tRNA-Val - tRNA with anticodon GAC for Val (VIMSS-AUTO), at 2,651,265 to 2,651,339
tRNA-Val
DVU2539: DVU2539 - hypothetical protein (TIGR), at 2,651,364 to 2,651,486
DVU2539
DVU2540: DVU2540 - lyase, putative (TIGR), at 2,651,492 to 2,652,634
DVU2540
DVU2541: DVU2541 - CoA-substrate-specific enzyme activase, putative (TIGR), at 2,652,642 to 2,653,451
DVU2541
Position (kb)
2651
2652
2653 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2651.462 kb on + strand, within DVU2539 at 2651.479 kb on + strand at 2651.529 kb on + strand at 2651.551 kb on + strand at 2651.559 kb on - strand at 2651.595 kb on - strand at 2651.611 kb on + strand, within DVU2540 at 2651.617 kb on - strand, within DVU2540 at 2651.634 kb on - strand, within DVU2540 at 2651.634 kb on - strand, within DVU2540 at 2651.720 kb on - strand, within DVU2540 at 2651.732 kb on - strand, within DVU2540 at 2651.742 kb on + strand, within DVU2540 at 2651.778 kb on + strand, within DVU2540 at 2651.811 kb on - strand, within DVU2540 at 2651.829 kb on - strand, within DVU2540 at 2651.843 kb on + strand, within DVU2540 at 2651.870 kb on - strand, within DVU2540 at 2651.920 kb on - strand, within DVU2540 at 2651.946 kb on - strand, within DVU2540 at 2651.948 kb on - strand, within DVU2540 at 2651.957 kb on + strand, within DVU2540 at 2652.009 kb on - strand, within DVU2540 at 2652.091 kb on - strand, within DVU2540 at 2652.139 kb on - strand, within DVU2540 at 2652.165 kb on - strand, within DVU2540 at 2652.186 kb on - strand, within DVU2540 at 2652.202 kb on + strand, within DVU2540 at 2652.225 kb on + strand, within DVU2540 at 2652.225 kb on + strand, within DVU2540 at 2652.241 kb on + strand, within DVU2540 at 2652.283 kb on - strand, within DVU2540 at 2652.487 kb on + strand, within DVU2540 at 2652.519 kb on + strand, within DVU2540 at 2652.546 kb on + strand at 2652.704 kb on - strand at 2652.745 kb on + strand, within DVU2541 at 2652.745 kb on + strand, within DVU2541 at 2652.847 kb on + strand, within DVU2541 at 2652.869 kb on + strand, within DVU2541 at 2652.900 kb on - strand, within DVU2541 at 2652.928 kb on + strand, within DVU2541 at 2652.936 kb on - strand, within DVU2541 at 2652.964 kb on + strand, within DVU2541 at 2652.977 kb on + strand, within DVU2541 at 2653.000 kb on - strand, within DVU2541 at 2653.010 kb on + strand, within DVU2541 at 2653.035 kb on + strand, within DVU2541 at 2653.043 kb on + strand, within DVU2541 at 2653.092 kb on + strand, within DVU2541 at 2653.102 kb on + strand, within DVU2541 at 2653.102 kb on + strand, within DVU2541 at 2653.102 kb on + strand, within DVU2541 at 2653.107 kb on + strand, within DVU2541 at 2653.126 kb on + strand, within DVU2541 at 2653.134 kb on + strand, within DVU2541 at 2653.203 kb on + strand, within DVU2541 at 2653.270 kb on + strand, within DVU2541 at 2653.320 kb on - strand, within DVU2541 at 2653.376 kb on + strand at 2653.377 kb on - strand at 2653.384 kb on - strand at 2653.388 kb on + strand at 2653.453 kb on + strand at 2653.470 kb on + strand at 2653.478 kb on - strand at 2653.478 kb on - strand at 2653.559 kb on + strand at 2653.559 kb on + strand at 2653.567 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.05% remove 2,651,462 + DVU2539 0.80 -0.1 2,651,479 + +0.7 2,651,529 + -0.5 2,651,551 + -0.1 2,651,559 - +0.1 2,651,595 - -0.4 2,651,611 + DVU2540 0.10 +0.8 2,651,617 - DVU2540 0.11 -0.0 2,651,634 - DVU2540 0.12 -0.3 2,651,634 - DVU2540 0.12 +1.0 2,651,720 - DVU2540 0.20 +0.1 2,651,732 - DVU2540 0.21 +2.0 2,651,742 + DVU2540 0.22 -0.0 2,651,778 + DVU2540 0.25 +1.5 2,651,811 - DVU2540 0.28 +0.3 2,651,829 - DVU2540 0.29 -0.0 2,651,843 + DVU2540 0.31 -0.0 2,651,870 - DVU2540 0.33 +0.6 2,651,920 - DVU2540 0.37 -0.1 2,651,946 - DVU2540 0.40 -0.0 2,651,948 - DVU2540 0.40 -0.0 2,651,957 + DVU2540 0.41 +2.0 2,652,009 - DVU2540 0.45 +0.2 2,652,091 - DVU2540 0.52 -0.1 2,652,139 - DVU2540 0.57 -0.6 2,652,165 - DVU2540 0.59 -0.4 2,652,186 - DVU2540 0.61 +1.0 2,652,202 + DVU2540 0.62 +0.0 2,652,225 + DVU2540 0.64 -1.4 2,652,225 + DVU2540 0.64 +0.3 2,652,241 + DVU2540 0.66 -0.4 2,652,283 - DVU2540 0.69 +0.8 2,652,487 + DVU2540 0.87 +2.0 2,652,519 + DVU2540 0.90 +0.5 2,652,546 + -0.0 2,652,704 - +0.1 2,652,745 + DVU2541 0.13 +0.0 2,652,745 + DVU2541 0.13 -0.5 2,652,847 + DVU2541 0.25 -0.6 2,652,869 + DVU2541 0.28 +1.0 2,652,900 - DVU2541 0.32 -3.0 2,652,928 + DVU2541 0.35 -1.0 2,652,936 - DVU2541 0.36 -0.6 2,652,964 + DVU2541 0.40 -0.0 2,652,977 + DVU2541 0.41 -0.0 2,653,000 - DVU2541 0.44 -0.5 2,653,010 + DVU2541 0.45 -0.4 2,653,035 + DVU2541 0.49 +1.2 2,653,043 + DVU2541 0.50 -0.0 2,653,092 + DVU2541 0.56 +1.0 2,653,102 + DVU2541 0.57 +0.7 2,653,102 + DVU2541 0.57 -0.3 2,653,102 + DVU2541 0.57 -0.4 2,653,107 + DVU2541 0.57 -0.4 2,653,126 + DVU2541 0.60 -0.0 2,653,134 + DVU2541 0.61 -0.5 2,653,203 + DVU2541 0.69 -0.5 2,653,270 + DVU2541 0.78 -0.1 2,653,320 - DVU2541 0.84 -0.4 2,653,376 + +0.1 2,653,377 - +0.1 2,653,384 - +1.0 2,653,388 + +0.3 2,653,453 + -0.1 2,653,470 + +0.0 2,653,478 - +1.4 2,653,478 - -0.8 2,653,559 + -0.5 2,653,559 + +2.0 2,653,567 - -0.4
Or see this region's nucleotide sequence