Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_4176 and HP15_4177 are separated by 80 nucleotides HP15_4177 and HP15_4178 are separated by 16 nucleotides HP15_4178 and HP15_4179 overlap by 1 nucleotides
HP15_4176: HP15_4176 - electron-transferring-flavoprotein dehydrogenase, at 4,414,535 to 4,416,187
_4176
HP15_4177: HP15_4177 - acetyl-CoA synthetase, at 4,416,268 to 4,418,256
_4177
HP15_4178: HP15_4178 - pyruvate carboxyltransferase, at 4,418,273 to 4,419,184
_4178
HP15_4179: HP15_4179 - methylcrotonyl-CoA carboxylase, alpha subunit, at 4,419,184 to 4,421,178
_4179
Position (kb)
4416
4417
4418
4419 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4415.667 kb on - strand, within HP15_4176 at 4416.320 kb on + strand at 4416.325 kb on + strand at 4416.333 kb on - strand at 4416.333 kb on - strand at 4416.391 kb on - strand at 4416.414 kb on + strand at 4416.450 kb on + strand at 4416.597 kb on + strand, within HP15_4177 at 4416.613 kb on - strand, within HP15_4177 at 4416.654 kb on + strand, within HP15_4177 at 4416.711 kb on + strand, within HP15_4177 at 4416.735 kb on + strand, within HP15_4177 at 4416.737 kb on + strand, within HP15_4177 at 4416.754 kb on - strand, within HP15_4177 at 4416.769 kb on - strand, within HP15_4177 at 4416.832 kb on - strand, within HP15_4177 at 4416.838 kb on + strand, within HP15_4177 at 4416.846 kb on - strand, within HP15_4177 at 4416.872 kb on + strand, within HP15_4177 at 4417.486 kb on + strand, within HP15_4177 at 4417.586 kb on + strand, within HP15_4177 at 4417.593 kb on + strand, within HP15_4177 at 4417.773 kb on - strand, within HP15_4177 at 4417.773 kb on - strand, within HP15_4177 at 4417.893 kb on - strand, within HP15_4177 at 4417.893 kb on - strand, within HP15_4177 at 4417.912 kb on + strand, within HP15_4177 at 4417.975 kb on + strand, within HP15_4177 at 4417.975 kb on + strand, within HP15_4177 at 4418.021 kb on - strand, within HP15_4177 at 4418.069 kb on + strand at 4418.098 kb on - strand at 4418.121 kb on - strand at 4418.157 kb on + strand at 4418.157 kb on + strand at 4418.165 kb on - strand at 4418.176 kb on - strand at 4418.450 kb on - strand, within HP15_4178 at 4418.461 kb on - strand, within HP15_4178 at 4418.595 kb on - strand, within HP15_4178 at 4418.710 kb on + strand, within HP15_4178 at 4418.813 kb on + strand, within HP15_4178 at 4418.952 kb on - strand, within HP15_4178 at 4419.061 kb on - strand, within HP15_4178
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 4,415,667 - HP15_4176 0.68 +1.5 4,416,320 + -2.9 4,416,325 + +0.6 4,416,333 - +0.5 4,416,333 - -0.1 4,416,391 - -0.1 4,416,414 + +0.2 4,416,450 + +0.7 4,416,597 + HP15_4177 0.17 +1.5 4,416,613 - HP15_4177 0.17 +1.5 4,416,654 + HP15_4177 0.19 +0.4 4,416,711 + HP15_4177 0.22 +0.4 4,416,735 + HP15_4177 0.23 +0.1 4,416,737 + HP15_4177 0.24 -0.2 4,416,754 - HP15_4177 0.24 +0.0 4,416,769 - HP15_4177 0.25 +0.2 4,416,832 - HP15_4177 0.28 -1.4 4,416,838 + HP15_4177 0.29 +0.3 4,416,846 - HP15_4177 0.29 -0.1 4,416,872 + HP15_4177 0.30 +0.7 4,417,486 + HP15_4177 0.61 +0.7 4,417,586 + HP15_4177 0.66 -0.5 4,417,593 + HP15_4177 0.67 +1.2 4,417,773 - HP15_4177 0.76 +0.4 4,417,773 - HP15_4177 0.76 -0.3 4,417,893 - HP15_4177 0.82 +0.5 4,417,893 - HP15_4177 0.82 +0.5 4,417,912 + HP15_4177 0.83 +0.2 4,417,975 + HP15_4177 0.86 -1.4 4,417,975 + HP15_4177 0.86 -0.0 4,418,021 - HP15_4177 0.88 +0.9 4,418,069 + -0.0 4,418,098 - +0.2 4,418,121 - -0.7 4,418,157 + -0.7 4,418,157 + -1.0 4,418,165 - +0.7 4,418,176 - +0.4 4,418,450 - HP15_4178 0.19 +0.3 4,418,461 - HP15_4178 0.21 +1.0 4,418,595 - HP15_4178 0.35 +0.0 4,418,710 + HP15_4178 0.48 +0.7 4,418,813 + HP15_4178 0.59 +0.6 4,418,952 - HP15_4178 0.74 +0.1 4,419,061 - HP15_4178 0.86 -0.0
Or see this region's nucleotide sequence