Experiment: D-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3446 and HP15_3447 are separated by 193 nucleotides HP15_3447 and HP15_3448 are separated by 3 nucleotides HP15_3448 and HP15_3449 are separated by 18 nucleotides
HP15_3446: HP15_3446 - protein containing a von Willebrand factor type A (vWA) domain, at 3,623,632 to 3,625,746
_3446
HP15_3447: HP15_3447 - two component transcriptional regulator, winged helix family, at 3,625,940 to 3,626,641
_3447
HP15_3448: HP15_3448 - integral membrane sensor signal transduction histidine kinase, at 3,626,645 to 3,628,675
_3448
HP15_3449: HP15_3449 - conserved hypothetical protein, membrane, at 3,628,694 to 3,630,001
_3449
Position (kb)
3626
3627
3628
3629 Strain fitness (log2 ratio)
-2
-1
0
1 at 3625.659 kb on + strand at 3625.664 kb on + strand at 3625.667 kb on - strand at 3625.667 kb on - strand at 3625.763 kb on + strand at 3625.763 kb on + strand at 3625.765 kb on + strand at 3625.771 kb on - strand at 3625.781 kb on - strand at 3626.008 kb on - strand at 3626.146 kb on + strand, within HP15_3447 at 3626.304 kb on - strand, within HP15_3447 at 3626.304 kb on - strand, within HP15_3447 at 3626.317 kb on + strand, within HP15_3447 at 3626.443 kb on + strand, within HP15_3447 at 3626.814 kb on - strand at 3626.871 kb on + strand, within HP15_3448 at 3626.906 kb on + strand, within HP15_3448 at 3626.914 kb on - strand, within HP15_3448 at 3627.049 kb on + strand, within HP15_3448 at 3627.063 kb on + strand, within HP15_3448 at 3627.071 kb on - strand, within HP15_3448 at 3627.074 kb on + strand, within HP15_3448 at 3627.096 kb on + strand, within HP15_3448 at 3627.104 kb on - strand, within HP15_3448 at 3627.142 kb on + strand, within HP15_3448 at 3627.142 kb on + strand, within HP15_3448 at 3627.227 kb on - strand, within HP15_3448 at 3627.316 kb on - strand, within HP15_3448 at 3627.383 kb on - strand, within HP15_3448 at 3627.510 kb on + strand, within HP15_3448 at 3627.543 kb on + strand, within HP15_3448 at 3627.560 kb on + strand, within HP15_3448 at 3627.561 kb on - strand, within HP15_3448 at 3627.644 kb on + strand, within HP15_3448 at 3627.725 kb on - strand, within HP15_3448 at 3627.759 kb on - strand, within HP15_3448 at 3627.761 kb on + strand, within HP15_3448 at 3627.773 kb on + strand, within HP15_3448 at 3627.931 kb on - strand, within HP15_3448 at 3628.193 kb on - strand, within HP15_3448 at 3628.340 kb on - strand, within HP15_3448 at 3628.374 kb on - strand, within HP15_3448 at 3628.436 kb on + strand, within HP15_3448 at 3628.444 kb on - strand, within HP15_3448 at 3628.451 kb on + strand, within HP15_3448 at 3628.464 kb on + strand, within HP15_3448 at 3628.472 kb on - strand, within HP15_3448 at 3628.472 kb on - strand, within HP15_3448 at 3628.472 kb on - strand, within HP15_3448 at 3628.527 kb on + strand at 3628.527 kb on + strand at 3628.535 kb on - strand at 3628.540 kb on - strand at 3628.550 kb on - strand at 3628.566 kb on - strand at 3628.627 kb on + strand at 3628.643 kb on - strand at 3628.645 kb on - strand at 3628.694 kb on - strand at 3628.719 kb on + strand at 3628.719 kb on + strand at 3628.727 kb on - strand at 3628.759 kb on - strand at 3628.766 kb on - strand at 3628.829 kb on - strand, within HP15_3449 at 3628.908 kb on + strand, within HP15_3449 at 3628.908 kb on + strand, within HP15_3449 at 3628.916 kb on - strand, within HP15_3449 at 3629.022 kb on + strand, within HP15_3449 at 3629.038 kb on - strand, within HP15_3449 at 3629.123 kb on + strand, within HP15_3449 at 3629.175 kb on + strand, within HP15_3449 at 3629.196 kb on + strand, within HP15_3449 at 3629.196 kb on + strand, within HP15_3449 at 3629.229 kb on + strand, within HP15_3449 at 3629.237 kb on - strand, within HP15_3449 at 3629.247 kb on - strand, within HP15_3449 at 3629.254 kb on - strand, within HP15_3449 at 3629.273 kb on + strand, within HP15_3449 at 3629.535 kb on + strand, within HP15_3449 at 3629.540 kb on + strand, within HP15_3449 at 3629.540 kb on + strand, within HP15_3449 at 3629.548 kb on - strand, within HP15_3449 at 3629.548 kb on - strand, within HP15_3449 at 3629.586 kb on + strand, within HP15_3449 at 3629.603 kb on + strand, within HP15_3449
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine remove 3,625,659 + -0.1 3,625,664 + -0.3 3,625,667 - -0.0 3,625,667 - +1.5 3,625,763 + -1.2 3,625,763 + -0.2 3,625,765 + +1.0 3,625,771 - -1.2 3,625,781 - -0.1 3,626,008 - +0.1 3,626,146 + HP15_3447 0.29 +1.2 3,626,304 - HP15_3447 0.52 +0.1 3,626,304 - HP15_3447 0.52 -1.4 3,626,317 + HP15_3447 0.54 -1.4 3,626,443 + HP15_3447 0.72 -1.7 3,626,814 - -0.5 3,626,871 + HP15_3448 0.11 -0.5 3,626,906 + HP15_3448 0.13 -0.3 3,626,914 - HP15_3448 0.13 +0.9 3,627,049 + HP15_3448 0.20 -0.9 3,627,063 + HP15_3448 0.21 +0.3 3,627,071 - HP15_3448 0.21 -1.1 3,627,074 + HP15_3448 0.21 -0.1 3,627,096 + HP15_3448 0.22 -0.0 3,627,104 - HP15_3448 0.23 -0.3 3,627,142 + HP15_3448 0.24 -0.6 3,627,142 + HP15_3448 0.24 -0.4 3,627,227 - HP15_3448 0.29 +0.4 3,627,316 - HP15_3448 0.33 +0.4 3,627,383 - HP15_3448 0.36 +0.1 3,627,510 + HP15_3448 0.43 -0.1 3,627,543 + HP15_3448 0.44 -1.1 3,627,560 + HP15_3448 0.45 -0.8 3,627,561 - HP15_3448 0.45 -0.6 3,627,644 + HP15_3448 0.49 -2.7 3,627,725 - HP15_3448 0.53 -0.5 3,627,759 - HP15_3448 0.55 -0.5 3,627,761 + HP15_3448 0.55 -1.1 3,627,773 + HP15_3448 0.56 -0.1 3,627,931 - HP15_3448 0.63 +0.5 3,628,193 - HP15_3448 0.76 -0.1 3,628,340 - HP15_3448 0.83 -2.1 3,628,374 - HP15_3448 0.85 -0.5 3,628,436 + HP15_3448 0.88 +0.8 3,628,444 - HP15_3448 0.89 +0.3 3,628,451 + HP15_3448 0.89 -0.2 3,628,464 + HP15_3448 0.90 -0.2 3,628,472 - HP15_3448 0.90 -0.6 3,628,472 - HP15_3448 0.90 -1.1 3,628,472 - HP15_3448 0.90 -0.7 3,628,527 + +0.5 3,628,527 + -0.1 3,628,535 - -0.2 3,628,540 - +0.3 3,628,550 - -1.4 3,628,566 - -0.9 3,628,627 + +0.7 3,628,643 - +1.3 3,628,645 - -0.1 3,628,694 - +0.9 3,628,719 + +0.7 3,628,719 + -1.3 3,628,727 - +1.5 3,628,759 - +0.2 3,628,766 - -1.7 3,628,829 - HP15_3449 0.10 +0.9 3,628,908 + HP15_3449 0.16 +0.1 3,628,908 + HP15_3449 0.16 -0.2 3,628,916 - HP15_3449 0.17 +0.2 3,629,022 + HP15_3449 0.25 -0.3 3,629,038 - HP15_3449 0.26 -0.1 3,629,123 + HP15_3449 0.33 +0.7 3,629,175 + HP15_3449 0.37 +0.5 3,629,196 + HP15_3449 0.38 +1.0 3,629,196 + HP15_3449 0.38 +0.6 3,629,229 + HP15_3449 0.41 +0.0 3,629,237 - HP15_3449 0.42 +0.2 3,629,247 - HP15_3449 0.42 -0.4 3,629,254 - HP15_3449 0.43 -0.2 3,629,273 + HP15_3449 0.44 +0.5 3,629,535 + HP15_3449 0.64 -0.2 3,629,540 + HP15_3449 0.65 +0.5 3,629,540 + HP15_3449 0.65 +0.9 3,629,548 - HP15_3449 0.65 +1.1 3,629,548 - HP15_3449 0.65 +0.9 3,629,586 + HP15_3449 0.68 +0.4 3,629,603 + HP15_3449 0.69 -0.3
Or see this region's nucleotide sequence