Experiment: reconstituted MRS with Sialyllacto-N-tetraose c 5.0 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16615 and BBR_RS16620 overlap by 11 nucleotides BBR_RS16620 and BBR_RS16625 are separated by 15 nucleotides BBR_RS16625 and BBR_RS16630 are separated by 124 nucleotides
BBR_RS16615: BBR_RS16615 - class I SAM-dependent RNA methyltransferase, at 1,496,479 to 1,497,738
_RS16615
BBR_RS16620: BBR_RS16620 - DUF3159 domain-containing protein, at 1,497,728 to 1,498,504
_RS16620
BBR_RS16625: BBR_RS16625 - DUF3710 domain-containing protein, at 1,498,520 to 1,499,365
_RS16625
BBR_RS16630: BBR_RS16630 - exodeoxyribonuclease III, at 1,499,490 to 1,500,350
_RS16630
Position (kb)
1498
1499
1500 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1497.527 kb on + strand, within BBR_RS16615 at 1497.535 kb on - strand, within BBR_RS16615 at 1497.555 kb on - strand, within BBR_RS16615 at 1497.584 kb on + strand, within BBR_RS16615 at 1497.584 kb on + strand, within BBR_RS16615 at 1497.621 kb on + strand at 1497.629 kb on - strand at 1497.681 kb on - strand at 1497.692 kb on - strand at 1497.697 kb on + strand at 1497.746 kb on - strand at 1497.903 kb on + strand, within BBR_RS16620 at 1497.911 kb on - strand, within BBR_RS16620 at 1497.925 kb on - strand, within BBR_RS16620 at 1497.959 kb on + strand, within BBR_RS16620 at 1498.012 kb on - strand, within BBR_RS16620 at 1498.012 kb on - strand, within BBR_RS16620 at 1498.041 kb on - strand, within BBR_RS16620 at 1498.116 kb on - strand, within BBR_RS16620 at 1498.193 kb on - strand, within BBR_RS16620 at 1498.221 kb on - strand, within BBR_RS16620 at 1498.240 kb on - strand, within BBR_RS16620 at 1498.267 kb on + strand, within BBR_RS16620 at 1498.388 kb on - strand, within BBR_RS16620 at 1498.483 kb on - strand at 1498.513 kb on + strand at 1498.596 kb on + strand at 1498.646 kb on - strand, within BBR_RS16625 at 1498.727 kb on - strand, within BBR_RS16625 at 1498.773 kb on + strand, within BBR_RS16625 at 1498.818 kb on - strand, within BBR_RS16625 at 1498.947 kb on - strand, within BBR_RS16625 at 1498.958 kb on + strand, within BBR_RS16625 at 1498.966 kb on - strand, within BBR_RS16625 at 1498.966 kb on - strand, within BBR_RS16625 at 1498.983 kb on - strand, within BBR_RS16625 at 1498.995 kb on - strand, within BBR_RS16625 at 1499.068 kb on - strand, within BBR_RS16625 at 1499.087 kb on + strand, within BBR_RS16625 at 1499.119 kb on + strand, within BBR_RS16625 at 1499.187 kb on - strand, within BBR_RS16625 at 1499.215 kb on - strand, within BBR_RS16625 at 1499.259 kb on + strand, within BBR_RS16625 at 1499.267 kb on - strand, within BBR_RS16625 at 1499.267 kb on - strand, within BBR_RS16625 at 1499.395 kb on - strand at 1499.423 kb on - strand at 1499.500 kb on + strand at 1499.517 kb on - strand at 1499.548 kb on - strand at 1499.601 kb on - strand, within BBR_RS16630 at 1499.610 kb on + strand, within BBR_RS16630 at 1499.610 kb on + strand, within BBR_RS16630 at 1499.610 kb on + strand, within BBR_RS16630 at 1499.610 kb on + strand, within BBR_RS16630 at 1499.610 kb on + strand, within BBR_RS16630 at 1499.618 kb on - strand, within BBR_RS16630 at 1499.782 kb on + strand, within BBR_RS16630 at 1499.782 kb on + strand, within BBR_RS16630 at 1499.901 kb on - strand, within BBR_RS16630 at 1499.948 kb on - strand, within BBR_RS16630 at 1500.140 kb on - strand, within BBR_RS16630 at 1500.185 kb on - strand, within BBR_RS16630 at 1500.199 kb on + strand, within BBR_RS16630 at 1500.254 kb on + strand, within BBR_RS16630 at 1500.254 kb on + strand, within BBR_RS16630 at 1500.295 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Sialyllacto-N-tetraose c 5.0 mM remove 1,497,527 + BBR_RS16615 0.83 +0.8 1,497,535 - BBR_RS16615 0.84 -0.3 1,497,555 - BBR_RS16615 0.85 +0.5 1,497,584 + BBR_RS16615 0.88 +0.2 1,497,584 + BBR_RS16615 0.88 -1.4 1,497,621 + +0.9 1,497,629 - -0.4 1,497,681 - -0.3 1,497,692 - -0.8 1,497,697 + -0.5 1,497,746 - +0.9 1,497,903 + BBR_RS16620 0.23 +0.1 1,497,911 - BBR_RS16620 0.24 +0.3 1,497,925 - BBR_RS16620 0.25 -0.5 1,497,959 + BBR_RS16620 0.30 -0.8 1,498,012 - BBR_RS16620 0.37 -0.8 1,498,012 - BBR_RS16620 0.37 -0.5 1,498,041 - BBR_RS16620 0.40 -0.1 1,498,116 - BBR_RS16620 0.50 +0.8 1,498,193 - BBR_RS16620 0.60 +0.9 1,498,221 - BBR_RS16620 0.63 -0.2 1,498,240 - BBR_RS16620 0.66 +0.3 1,498,267 + BBR_RS16620 0.69 -0.0 1,498,388 - BBR_RS16620 0.85 -0.1 1,498,483 - +1.0 1,498,513 + +0.2 1,498,596 + -0.1 1,498,646 - BBR_RS16625 0.15 +0.1 1,498,727 - BBR_RS16625 0.24 +0.3 1,498,773 + BBR_RS16625 0.30 +0.8 1,498,818 - BBR_RS16625 0.35 +0.2 1,498,947 - BBR_RS16625 0.50 +1.0 1,498,958 + BBR_RS16625 0.52 +0.3 1,498,966 - BBR_RS16625 0.53 -0.5 1,498,966 - BBR_RS16625 0.53 +0.5 1,498,983 - BBR_RS16625 0.55 -0.1 1,498,995 - BBR_RS16625 0.56 -0.2 1,499,068 - BBR_RS16625 0.65 -1.2 1,499,087 + BBR_RS16625 0.67 -1.0 1,499,119 + BBR_RS16625 0.71 -0.9 1,499,187 - BBR_RS16625 0.79 +0.0 1,499,215 - BBR_RS16625 0.82 +1.0 1,499,259 + BBR_RS16625 0.87 +2.9 1,499,267 - BBR_RS16625 0.88 -0.6 1,499,267 - BBR_RS16625 0.88 -1.0 1,499,395 - +0.6 1,499,423 - +0.0 1,499,500 + +0.7 1,499,517 - +0.1 1,499,548 - +1.0 1,499,601 - BBR_RS16630 0.13 -0.1 1,499,610 + BBR_RS16630 0.14 -0.9 1,499,610 + BBR_RS16630 0.14 +0.0 1,499,610 + BBR_RS16630 0.14 -0.9 1,499,610 + BBR_RS16630 0.14 +0.7 1,499,610 + BBR_RS16630 0.14 +0.3 1,499,618 - BBR_RS16630 0.15 +0.2 1,499,782 + BBR_RS16630 0.34 +0.1 1,499,782 + BBR_RS16630 0.34 -1.2 1,499,901 - BBR_RS16630 0.48 -0.7 1,499,948 - BBR_RS16630 0.53 +0.2 1,500,140 - BBR_RS16630 0.75 -0.2 1,500,185 - BBR_RS16630 0.81 -0.0 1,500,199 + BBR_RS16630 0.82 +0.5 1,500,254 + BBR_RS16630 0.89 +0.0 1,500,254 + BBR_RS16630 0.89 +0.1 1,500,295 - -0.9
Or see this region's nucleotide sequence