Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4959

Experiment: phosphite 0.25 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4958 and Pf6N2E2_4959 are separated by 151 nucleotidesPf6N2E2_4959 and Pf6N2E2_4960 are separated by 59 nucleotidesPf6N2E2_4960 and Pf6N2E2_4961 are separated by 30 nucleotides Pf6N2E2_4958 - Cystathionine gamma-synthase (EC 2.5.1.48), at 5,717,442 to 5,718,695 _4958 Pf6N2E2_4959 - S-methylmethionine permease, at 5,718,847 to 5,720,277 _4959 Pf6N2E2_4960 - Transcriptional regulator, LysR family, at 5,720,337 to 5,721,221 _4960 Pf6N2E2_4961 - Threonine synthase (EC 4.2.3.1), at 5,721,252 to 5,722,616 _4961 Position (kb) 5718 5719 5720 5721Strain fitness (log2 ratio) -2 -1 0 1 2at 5717.920 kb on - strand, within Pf6N2E2_4958at 5717.920 kb on - strand, within Pf6N2E2_4958at 5718.077 kb on + strand, within Pf6N2E2_4958at 5718.077 kb on + strand, within Pf6N2E2_4958at 5718.078 kb on - strand, within Pf6N2E2_4958at 5718.207 kb on + strand, within Pf6N2E2_4958at 5718.492 kb on + strand, within Pf6N2E2_4958at 5718.492 kb on + strand, within Pf6N2E2_4958at 5718.492 kb on + strand, within Pf6N2E2_4958at 5718.492 kb on + strand, within Pf6N2E2_4958at 5718.492 kb on + strand, within Pf6N2E2_4958at 5718.492 kb on + strand, within Pf6N2E2_4958at 5718.493 kb on - strand, within Pf6N2E2_4958at 5718.493 kb on - strand, within Pf6N2E2_4958at 5718.493 kb on - strand, within Pf6N2E2_4958at 5718.493 kb on - strand, within Pf6N2E2_4958at 5718.493 kb on - strand, within Pf6N2E2_4958at 5718.607 kb on + strandat 5718.608 kb on - strandat 5718.608 kb on - strandat 5718.608 kb on - strandat 5718.608 kb on - strandat 5718.608 kb on - strandat 5718.747 kb on + strandat 5718.757 kb on - strandat 5718.767 kb on - strandat 5718.783 kb on + strandat 5718.796 kb on - strandat 5718.796 kb on - strandat 5718.812 kb on + strandat 5718.824 kb on + strandat 5718.912 kb on + strandat 5718.912 kb on + strandat 5718.973 kb on - strandat 5718.973 kb on - strandat 5719.012 kb on + strand, within Pf6N2E2_4959at 5719.013 kb on - strand, within Pf6N2E2_4959at 5719.013 kb on - strand, within Pf6N2E2_4959at 5719.036 kb on + strand, within Pf6N2E2_4959at 5719.036 kb on + strand, within Pf6N2E2_4959at 5719.036 kb on + strand, within Pf6N2E2_4959at 5719.036 kb on + strand, within Pf6N2E2_4959at 5719.036 kb on + strand, within Pf6N2E2_4959at 5719.037 kb on - strand, within Pf6N2E2_4959at 5719.037 kb on - strand, within Pf6N2E2_4959at 5719.037 kb on - strand, within Pf6N2E2_4959at 5719.102 kb on + strand, within Pf6N2E2_4959at 5719.102 kb on + strand, within Pf6N2E2_4959at 5719.102 kb on + strand, within Pf6N2E2_4959at 5719.103 kb on - strand, within Pf6N2E2_4959at 5719.103 kb on - strand, within Pf6N2E2_4959at 5719.135 kb on + strand, within Pf6N2E2_4959at 5719.135 kb on + strand, within Pf6N2E2_4959at 5719.153 kb on + strand, within Pf6N2E2_4959at 5719.154 kb on - strand, within Pf6N2E2_4959at 5719.154 kb on - strand, within Pf6N2E2_4959at 5719.565 kb on + strand, within Pf6N2E2_4959at 5719.565 kb on + strand, within Pf6N2E2_4959at 5719.565 kb on + strand, within Pf6N2E2_4959at 5719.565 kb on + strand, within Pf6N2E2_4959at 5719.566 kb on - strand, within Pf6N2E2_4959at 5719.566 kb on - strand, within Pf6N2E2_4959at 5719.696 kb on + strand, within Pf6N2E2_4959at 5719.696 kb on + strand, within Pf6N2E2_4959at 5719.696 kb on + strand, within Pf6N2E2_4959at 5719.697 kb on - strand, within Pf6N2E2_4959at 5719.697 kb on - strand, within Pf6N2E2_4959at 5719.697 kb on - strand, within Pf6N2E2_4959at 5719.697 kb on - strand, within Pf6N2E2_4959at 5719.712 kb on - strand, within Pf6N2E2_4959at 5719.777 kb on + strand, within Pf6N2E2_4959at 5719.777 kb on + strand, within Pf6N2E2_4959at 5719.778 kb on - strand, within Pf6N2E2_4959at 5719.778 kb on - strand, within Pf6N2E2_4959at 5719.939 kb on + strand, within Pf6N2E2_4959at 5719.939 kb on + strand, within Pf6N2E2_4959at 5719.939 kb on + strand, within Pf6N2E2_4959at 5719.940 kb on - strand, within Pf6N2E2_4959at 5719.940 kb on - strand, within Pf6N2E2_4959at 5719.940 kb on - strand, within Pf6N2E2_4959at 5720.026 kb on + strand, within Pf6N2E2_4959at 5720.026 kb on + strand, within Pf6N2E2_4959at 5720.026 kb on + strand, within Pf6N2E2_4959at 5720.026 kb on + strand, within Pf6N2E2_4959at 5720.026 kb on + strand, within Pf6N2E2_4959at 5720.026 kb on + strand, within Pf6N2E2_4959at 5720.026 kb on + strand, within Pf6N2E2_4959at 5720.026 kb on + strand, within Pf6N2E2_4959at 5720.026 kb on + strand, within Pf6N2E2_4959at 5720.026 kb on + strand, within Pf6N2E2_4959at 5720.027 kb on - strand, within Pf6N2E2_4959at 5720.027 kb on - strand, within Pf6N2E2_4959at 5720.027 kb on - strand, within Pf6N2E2_4959at 5720.027 kb on - strand, within Pf6N2E2_4959at 5720.027 kb on - strand, within Pf6N2E2_4959at 5720.027 kb on - strand, within Pf6N2E2_4959at 5720.027 kb on - strand, within Pf6N2E2_4959at 5720.027 kb on - strand, within Pf6N2E2_4959at 5720.027 kb on - strand, within Pf6N2E2_4959at 5720.164 kb on + strandat 5720.194 kb on + strandat 5720.194 kb on + strandat 5720.194 kb on + strandat 5720.204 kb on - strandat 5720.204 kb on - strandat 5720.206 kb on + strandat 5720.206 kb on + strandat 5720.206 kb on + strandat 5720.206 kb on + strandat 5720.206 kb on + strandat 5720.206 kb on + strandat 5720.206 kb on + strandat 5720.206 kb on + strandat 5720.206 kb on + strandat 5720.206 kb on + strandat 5720.206 kb on + strandat 5720.207 kb on - strandat 5720.207 kb on - strandat 5720.207 kb on - strandat 5720.207 kb on - strandat 5720.207 kb on - strandat 5720.207 kb on - strandat 5720.211 kb on + strandat 5720.275 kb on + strandat 5720.276 kb on - strandat 5720.276 kb on - strandat 5720.276 kb on - strandat 5720.276 kb on - strandat 5720.544 kb on + strand, within Pf6N2E2_4960at 5720.545 kb on - strand, within Pf6N2E2_4960at 5720.719 kb on - strand, within Pf6N2E2_4960at 5720.719 kb on - strand, within Pf6N2E2_4960at 5720.916 kb on + strand, within Pf6N2E2_4960at 5720.935 kb on - strand, within Pf6N2E2_4960at 5721.132 kb on + strand, within Pf6N2E2_4960at 5721.132 kb on + strand, within Pf6N2E2_4960at 5721.132 kb on + strand, within Pf6N2E2_4960at 5721.133 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction phosphite 0.25 M
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5,717,920 - Pf6N2E2_4958 0.38 +0.9
5,717,920 - Pf6N2E2_4958 0.38 +0.8
5,718,077 + Pf6N2E2_4958 0.51 +0.3
5,718,077 + Pf6N2E2_4958 0.51 +0.1
5,718,078 - Pf6N2E2_4958 0.51 +0.9
5,718,207 + Pf6N2E2_4958 0.61 +1.3
5,718,492 + Pf6N2E2_4958 0.84 +0.5
5,718,492 + Pf6N2E2_4958 0.84 +1.1
5,718,492 + Pf6N2E2_4958 0.84 -1.3
5,718,492 + Pf6N2E2_4958 0.84 +0.3
5,718,492 + Pf6N2E2_4958 0.84 +0.5
5,718,492 + Pf6N2E2_4958 0.84 -0.7
5,718,493 - Pf6N2E2_4958 0.84 -2.3
5,718,493 - Pf6N2E2_4958 0.84 -0.0
5,718,493 - Pf6N2E2_4958 0.84 -0.3
5,718,493 - Pf6N2E2_4958 0.84 -0.3
5,718,493 - Pf6N2E2_4958 0.84 +0.5
5,718,607 + +0.2
5,718,608 - +0.5
5,718,608 - +0.1
5,718,608 - +0.7
5,718,608 - +0.1
5,718,608 - -0.3
5,718,747 + -1.8
5,718,757 - -0.0
5,718,767 - +0.8
5,718,783 + -0.2
5,718,796 - +0.3
5,718,796 - +0.3
5,718,812 + -0.8
5,718,824 + -0.2
5,718,912 + -1.0
5,718,912 + -0.4
5,718,973 - -0.4
5,718,973 - +1.9
5,719,012 + Pf6N2E2_4959 0.12 -0.9
5,719,013 - Pf6N2E2_4959 0.12 -1.4
5,719,013 - Pf6N2E2_4959 0.12 -1.5
5,719,036 + Pf6N2E2_4959 0.13 -0.4
5,719,036 + Pf6N2E2_4959 0.13 +0.6
5,719,036 + Pf6N2E2_4959 0.13 -0.7
5,719,036 + Pf6N2E2_4959 0.13 +0.2
5,719,036 + Pf6N2E2_4959 0.13 -0.3
5,719,037 - Pf6N2E2_4959 0.13 +0.5
5,719,037 - Pf6N2E2_4959 0.13 +2.7
5,719,037 - Pf6N2E2_4959 0.13 +0.5
5,719,102 + Pf6N2E2_4959 0.18 -2.4
5,719,102 + Pf6N2E2_4959 0.18 -0.4
5,719,102 + Pf6N2E2_4959 0.18 +1.1
5,719,103 - Pf6N2E2_4959 0.18 +0.6
5,719,103 - Pf6N2E2_4959 0.18 +0.2
5,719,135 + Pf6N2E2_4959 0.20 -0.4
5,719,135 + Pf6N2E2_4959 0.20 -0.5
5,719,153 + Pf6N2E2_4959 0.21 -0.3
5,719,154 - Pf6N2E2_4959 0.21 -0.9
5,719,154 - Pf6N2E2_4959 0.21 +0.5
5,719,565 + Pf6N2E2_4959 0.50 +0.7
5,719,565 + Pf6N2E2_4959 0.50 -0.5
5,719,565 + Pf6N2E2_4959 0.50 -0.1
5,719,565 + Pf6N2E2_4959 0.50 -1.3
5,719,566 - Pf6N2E2_4959 0.50 -1.0
5,719,566 - Pf6N2E2_4959 0.50 +0.2
5,719,696 + Pf6N2E2_4959 0.59 -0.5
5,719,696 + Pf6N2E2_4959 0.59 -1.3
5,719,696 + Pf6N2E2_4959 0.59 +0.1
5,719,697 - Pf6N2E2_4959 0.59 -0.0
5,719,697 - Pf6N2E2_4959 0.59 -1.4
5,719,697 - Pf6N2E2_4959 0.59 +0.2
5,719,697 - Pf6N2E2_4959 0.59 -0.0
5,719,712 - Pf6N2E2_4959 0.60 +0.3
5,719,777 + Pf6N2E2_4959 0.65 +0.5
5,719,777 + Pf6N2E2_4959 0.65 +1.0
5,719,778 - Pf6N2E2_4959 0.65 -0.6
5,719,778 - Pf6N2E2_4959 0.65 -0.6
5,719,939 + Pf6N2E2_4959 0.76 -0.1
5,719,939 + Pf6N2E2_4959 0.76 -0.6
5,719,939 + Pf6N2E2_4959 0.76 -1.8
5,719,940 - Pf6N2E2_4959 0.76 +1.0
5,719,940 - Pf6N2E2_4959 0.76 +0.3
5,719,940 - Pf6N2E2_4959 0.76 -2.1
5,720,026 + Pf6N2E2_4959 0.82 -0.9
5,720,026 + Pf6N2E2_4959 0.82 -1.0
5,720,026 + Pf6N2E2_4959 0.82 -0.1
5,720,026 + Pf6N2E2_4959 0.82 -0.9
5,720,026 + Pf6N2E2_4959 0.82 -0.7
5,720,026 + Pf6N2E2_4959 0.82 -0.7
5,720,026 + Pf6N2E2_4959 0.82 +1.7
5,720,026 + Pf6N2E2_4959 0.82 -1.2
5,720,026 + Pf6N2E2_4959 0.82 -1.4
5,720,026 + Pf6N2E2_4959 0.82 -0.5
5,720,027 - Pf6N2E2_4959 0.82 -0.4
5,720,027 - Pf6N2E2_4959 0.82 -0.5
5,720,027 - Pf6N2E2_4959 0.82 -2.2
5,720,027 - Pf6N2E2_4959 0.82 +1.0
5,720,027 - Pf6N2E2_4959 0.82 +1.0
5,720,027 - Pf6N2E2_4959 0.82 +0.1
5,720,027 - Pf6N2E2_4959 0.82 -0.3
5,720,027 - Pf6N2E2_4959 0.82 -0.8
5,720,027 - Pf6N2E2_4959 0.82 -0.0
5,720,164 + +0.4
5,720,194 + +0.5
5,720,194 + +0.1
5,720,194 + +1.1
5,720,204 - +0.4
5,720,204 - -0.5
5,720,206 + -1.2
5,720,206 + -0.6
5,720,206 + -0.0
5,720,206 + +1.1
5,720,206 + -2.2
5,720,206 + -1.3
5,720,206 + +0.7
5,720,206 + -0.6
5,720,206 + -0.8
5,720,206 + -0.3
5,720,206 + -1.3
5,720,207 - -0.7
5,720,207 - -1.7
5,720,207 - +0.3
5,720,207 - +1.7
5,720,207 - +0.3
5,720,207 - -0.1
5,720,211 + -0.5
5,720,275 + -1.1
5,720,276 - +0.4
5,720,276 - -0.0
5,720,276 - +0.5
5,720,276 - +1.1
5,720,544 + Pf6N2E2_4960 0.23 -1.6
5,720,545 - Pf6N2E2_4960 0.24 +0.3
5,720,719 - Pf6N2E2_4960 0.43 -0.5
5,720,719 - Pf6N2E2_4960 0.43 +0.3
5,720,916 + Pf6N2E2_4960 0.65 -1.0
5,720,935 - Pf6N2E2_4960 0.68 -1.9
5,721,132 + Pf6N2E2_4960 0.90 -2.5
5,721,132 + Pf6N2E2_4960 0.90 -1.6
5,721,132 + Pf6N2E2_4960 0.90 -0.7
5,721,133 - +0.5

Or see this region's nucleotide sequence