Experiment: phosphite 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4581 and Pf6N2E2_4582 are separated by 155 nucleotides Pf6N2E2_4582 and Pf6N2E2_4583 are separated by 57 nucleotides
Pf6N2E2_4581 - FIG015547: peptidase, M16 family, at 5,311,596 to 5,312,951
_4581
Pf6N2E2_4582 - hypothetical protein, at 5,313,107 to 5,313,238
_4582
Pf6N2E2_4583 - Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1), at 5,313,296 to 5,314,729
_4583
Position (kb) 5313
5314 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5312.255 kb on - strand, within Pf6N2E2_4581 at 5312.330 kb on - strand, within Pf6N2E2_4581 at 5312.450 kb on - strand, within Pf6N2E2_4581 at 5312.608 kb on + strand, within Pf6N2E2_4581 at 5312.608 kb on + strand, within Pf6N2E2_4581 at 5312.609 kb on - strand, within Pf6N2E2_4581 at 5312.609 kb on - strand, within Pf6N2E2_4581 at 5312.609 kb on - strand, within Pf6N2E2_4581 at 5312.609 kb on - strand, within Pf6N2E2_4581 at 5312.611 kb on + strand, within Pf6N2E2_4581 at 5312.611 kb on + strand, within Pf6N2E2_4581 at 5312.612 kb on - strand, within Pf6N2E2_4581 at 5312.612 kb on - strand, within Pf6N2E2_4581 at 5312.761 kb on + strand, within Pf6N2E2_4581 at 5312.779 kb on + strand, within Pf6N2E2_4581 at 5312.779 kb on + strand, within Pf6N2E2_4581 at 5312.779 kb on + strand, within Pf6N2E2_4581 at 5313.011 kb on + strand at 5313.057 kb on + strand at 5313.240 kb on + strand at 5313.240 kb on + strand at 5313.240 kb on + strand at 5313.240 kb on + strand at 5313.244 kb on + strand at 5313.244 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction phosphite 0.25 M remove 5,312,255 - Pf6N2E2_4581 0.49 +1.2 5,312,330 - Pf6N2E2_4581 0.54 +1.1 5,312,450 - Pf6N2E2_4581 0.63 +0.0 5,312,608 + Pf6N2E2_4581 0.75 +0.5 5,312,608 + Pf6N2E2_4581 0.75 +0.3 5,312,609 - Pf6N2E2_4581 0.75 -0.1 5,312,609 - Pf6N2E2_4581 0.75 +1.1 5,312,609 - Pf6N2E2_4581 0.75 -0.0 5,312,609 - Pf6N2E2_4581 0.75 -0.8 5,312,611 + Pf6N2E2_4581 0.75 +0.6 5,312,611 + Pf6N2E2_4581 0.75 +0.0 5,312,612 - Pf6N2E2_4581 0.75 -0.7 5,312,612 - Pf6N2E2_4581 0.75 +0.1 5,312,761 + Pf6N2E2_4581 0.86 +1.3 5,312,779 + Pf6N2E2_4581 0.87 +0.4 5,312,779 + Pf6N2E2_4581 0.87 +0.8 5,312,779 + Pf6N2E2_4581 0.87 +0.9 5,313,011 + +1.3 5,313,057 + -0.8 5,313,240 + -3.1 5,313,240 + +0.5 5,313,240 + +0.6 5,313,240 + -0.8 5,313,244 + +0.0 5,313,244 + -1.0
Or see this region's nucleotide sequence