Experiment: phosphite 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4162 and Pf6N2E2_4163 are separated by 74 nucleotides Pf6N2E2_4163 and Pf6N2E2_4164 are separated by 59 nucleotides Pf6N2E2_4164 and Pf6N2E2_4165 are separated by 165 nucleotides
Pf6N2E2_4162 - DNA polymerase I (EC 2.7.7.7), at 4,843,318 to 4,846,086
_4162
Pf6N2E2_4163 - FIG00953549: hypothetical protein, at 4,846,161 to 4,846,448
_4163
Pf6N2E2_4164 - Homoserine kinase (EC 2.7.1.39), at 4,846,508 to 4,847,458
_4164
Pf6N2E2_4165 - Zinc ABC transporter, periplasmic-binding protein ZnuA, at 4,847,624 to 4,848,553
_4165
Position (kb)
4846
4847
4848 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 4846.121 kb on - strand at 4846.157 kb on + strand at 4846.157 kb on + strand at 4846.158 kb on - strand at 4846.166 kb on + strand at 4846.166 kb on + strand at 4846.166 kb on + strand at 4846.166 kb on + strand at 4846.167 kb on - strand at 4846.167 kb on - strand at 4846.171 kb on + strand at 4846.171 kb on + strand at 4846.171 kb on + strand at 4846.172 kb on - strand at 4846.280 kb on + strand, within Pf6N2E2_4163 at 4846.318 kb on - strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.353 kb on + strand, within Pf6N2E2_4163 at 4846.435 kb on + strand at 4846.435 kb on + strand at 4846.446 kb on + strand at 4846.446 kb on + strand at 4846.446 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.456 kb on + strand at 4847.457 kb on - strand at 4847.457 kb on - strand at 4847.457 kb on - strand at 4847.457 kb on - strand at 4847.457 kb on - strand at 4847.457 kb on - strand at 4847.457 kb on - strand at 4847.460 kb on + strand at 4847.460 kb on + strand at 4847.460 kb on + strand at 4847.460 kb on + strand at 4847.461 kb on - strand at 4847.461 kb on - strand at 4847.461 kb on - strand at 4847.461 kb on - strand at 4847.461 kb on - strand at 4847.461 kb on - strand at 4847.627 kb on + strand at 4847.627 kb on + strand at 4847.627 kb on + strand at 4847.627 kb on + strand at 4847.627 kb on + strand at 4847.628 kb on - strand at 4847.628 kb on - strand at 4847.628 kb on - strand at 4847.628 kb on - strand at 4847.682 kb on + strand at 4847.706 kb on + strand at 4847.706 kb on + strand at 4847.706 kb on + strand at 4847.706 kb on + strand at 4847.707 kb on - strand at 4847.907 kb on + strand, within Pf6N2E2_4165 at 4847.907 kb on + strand, within Pf6N2E2_4165 at 4847.907 kb on + strand, within Pf6N2E2_4165 at 4847.907 kb on + strand, within Pf6N2E2_4165 at 4847.907 kb on + strand, within Pf6N2E2_4165 at 4847.907 kb on + strand, within Pf6N2E2_4165 at 4847.907 kb on + strand, within Pf6N2E2_4165 at 4847.908 kb on - strand, within Pf6N2E2_4165 at 4847.908 kb on - strand, within Pf6N2E2_4165 at 4847.908 kb on - strand, within Pf6N2E2_4165 at 4847.923 kb on - strand, within Pf6N2E2_4165 at 4847.949 kb on + strand, within Pf6N2E2_4165 at 4847.950 kb on - strand, within Pf6N2E2_4165 at 4847.950 kb on - strand, within Pf6N2E2_4165 at 4848.322 kb on - strand, within Pf6N2E2_4165 at 4848.372 kb on + strand, within Pf6N2E2_4165 at 4848.373 kb on - strand, within Pf6N2E2_4165
Per-strain Table
Position Strand Gene LocusTag Fraction phosphite 0.25 M remove 4,846,121 - -0.3 4,846,157 + +0.8 4,846,157 + -0.2 4,846,158 - -0.2 4,846,166 + -0.0 4,846,166 + -0.1 4,846,166 + +0.4 4,846,166 + +0.5 4,846,167 - -0.9 4,846,167 - -1.1 4,846,171 + -1.4 4,846,171 + +0.2 4,846,171 + +0.9 4,846,172 - -0.8 4,846,280 + Pf6N2E2_4163 0.41 +0.0 4,846,318 - Pf6N2E2_4163 0.55 +0.4 4,846,353 + Pf6N2E2_4163 0.67 +0.4 4,846,353 + Pf6N2E2_4163 0.67 -0.3 4,846,353 + Pf6N2E2_4163 0.67 +0.2 4,846,353 + Pf6N2E2_4163 0.67 -0.0 4,846,353 + Pf6N2E2_4163 0.67 -0.4 4,846,435 + -0.2 4,846,435 + +0.2 4,846,446 + -0.3 4,846,446 + +0.4 4,846,446 + +0.5 4,847,456 + -0.0 4,847,456 + -1.3 4,847,456 + +0.6 4,847,456 + -1.7 4,847,456 + +1.2 4,847,456 + -0.4 4,847,456 + -0.5 4,847,456 + +1.1 4,847,456 + -0.7 4,847,456 + +0.4 4,847,457 - +1.2 4,847,457 - -0.1 4,847,457 - -0.9 4,847,457 - +0.3 4,847,457 - +0.4 4,847,457 - +0.3 4,847,457 - +0.6 4,847,460 + +0.0 4,847,460 + +0.3 4,847,460 + -0.5 4,847,460 + -1.1 4,847,461 - +0.7 4,847,461 - -0.7 4,847,461 - -0.1 4,847,461 - +1.0 4,847,461 - -0.5 4,847,461 - -1.4 4,847,627 + +0.1 4,847,627 + +0.3 4,847,627 + +0.7 4,847,627 + +0.2 4,847,627 + +0.5 4,847,628 - +0.2 4,847,628 - +0.1 4,847,628 - -0.2 4,847,628 - +1.0 4,847,682 + -0.3 4,847,706 + +0.3 4,847,706 + +1.1 4,847,706 + +0.2 4,847,706 + -0.1 4,847,707 - -1.4 4,847,907 + Pf6N2E2_4165 0.30 -0.7 4,847,907 + Pf6N2E2_4165 0.30 +0.8 4,847,907 + Pf6N2E2_4165 0.30 +0.4 4,847,907 + Pf6N2E2_4165 0.30 -0.4 4,847,907 + Pf6N2E2_4165 0.30 -0.1 4,847,907 + Pf6N2E2_4165 0.30 -0.2 4,847,907 + Pf6N2E2_4165 0.30 +0.2 4,847,908 - Pf6N2E2_4165 0.31 +0.1 4,847,908 - Pf6N2E2_4165 0.31 -0.2 4,847,908 - Pf6N2E2_4165 0.31 +0.5 4,847,923 - Pf6N2E2_4165 0.32 +0.2 4,847,949 + Pf6N2E2_4165 0.35 +1.3 4,847,950 - Pf6N2E2_4165 0.35 +0.8 4,847,950 - Pf6N2E2_4165 0.35 +0.6 4,848,322 - Pf6N2E2_4165 0.75 +4.4 4,848,372 + Pf6N2E2_4165 0.80 -0.5 4,848,373 - Pf6N2E2_4165 0.81 +0.8
Or see this region's nucleotide sequence