Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3082

Experiment: phosphite 0.25 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3080 and Pf6N2E2_3081 are separated by 126 nucleotidesPf6N2E2_3081 and Pf6N2E2_3082 are separated by 44 nucleotidesPf6N2E2_3082 and Pf6N2E2_3083 overlap by 12 nucleotides Pf6N2E2_3080 - Cysteine desulfurase CsdA-CsdE, sulfur acceptor protein CsdE, at 3,568,614 to 3,569,021 _3080 Pf6N2E2_3081 - HesA/MoeB/ThiF family protein related to EC-YgdL, at 3,569,148 to 3,569,966 _3081 Pf6N2E2_3082 - hypothetical protein, at 3,570,011 to 3,570,190 _3082 Pf6N2E2_3083 - N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18), at 3,570,179 to 3,571,330 _3083 Position (kb) 3570 3571Strain fitness (log2 ratio) -3 -2 -1 0 1at 3569.201 kb on + strandat 3569.380 kb on + strand, within Pf6N2E2_3081at 3569.380 kb on + strand, within Pf6N2E2_3081at 3569.381 kb on - strand, within Pf6N2E2_3081at 3569.381 kb on - strand, within Pf6N2E2_3081at 3569.433 kb on + strand, within Pf6N2E2_3081at 3569.433 kb on + strand, within Pf6N2E2_3081at 3569.433 kb on + strand, within Pf6N2E2_3081at 3569.434 kb on - strand, within Pf6N2E2_3081at 3569.434 kb on - strand, within Pf6N2E2_3081at 3569.626 kb on + strand, within Pf6N2E2_3081at 3569.626 kb on + strand, within Pf6N2E2_3081at 3569.626 kb on + strand, within Pf6N2E2_3081at 3569.627 kb on - strand, within Pf6N2E2_3081at 3569.627 kb on - strand, within Pf6N2E2_3081at 3569.918 kb on - strandat 3569.955 kb on - strandat 3569.989 kb on + strandat 3569.989 kb on + strandat 3569.990 kb on - strandat 3569.990 kb on - strandat 3569.990 kb on - strandat 3569.991 kb on + strandat 3569.992 kb on - strandat 3569.992 kb on - strandat 3569.992 kb on - strandat 3569.992 kb on - strandat 3570.100 kb on + strand, within Pf6N2E2_3082at 3570.101 kb on - strand, within Pf6N2E2_3082at 3570.102 kb on + strand, within Pf6N2E2_3082at 3570.102 kb on + strand, within Pf6N2E2_3082at 3570.103 kb on - strand, within Pf6N2E2_3082at 3570.107 kb on + strand, within Pf6N2E2_3082at 3570.107 kb on + strand, within Pf6N2E2_3082at 3570.107 kb on + strand, within Pf6N2E2_3082at 3570.107 kb on + strand, within Pf6N2E2_3082at 3570.141 kb on + strand, within Pf6N2E2_3082at 3570.161 kb on + strand, within Pf6N2E2_3082at 3570.161 kb on + strand, within Pf6N2E2_3082

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Per-strain Table

Position Strand Gene LocusTag Fraction phosphite 0.25 M
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3,569,201 + +1.0
3,569,380 + Pf6N2E2_3081 0.28 -0.1
3,569,380 + Pf6N2E2_3081 0.28 +0.7
3,569,381 - Pf6N2E2_3081 0.28 -1.0
3,569,381 - Pf6N2E2_3081 0.28 +0.0
3,569,433 + Pf6N2E2_3081 0.35 +0.9
3,569,433 + Pf6N2E2_3081 0.35 -0.2
3,569,433 + Pf6N2E2_3081 0.35 -0.4
3,569,434 - Pf6N2E2_3081 0.35 -0.3
3,569,434 - Pf6N2E2_3081 0.35 -0.3
3,569,626 + Pf6N2E2_3081 0.58 -0.1
3,569,626 + Pf6N2E2_3081 0.58 -2.6
3,569,626 + Pf6N2E2_3081 0.58 -2.7
3,569,627 - Pf6N2E2_3081 0.58 +0.6
3,569,627 - Pf6N2E2_3081 0.58 -0.9
3,569,918 - +0.1
3,569,955 - -1.2
3,569,989 + +0.2
3,569,989 + +0.1
3,569,990 - +0.4
3,569,990 - +0.7
3,569,990 - +0.4
3,569,991 + -1.3
3,569,992 - +0.2
3,569,992 - +0.4
3,569,992 - -1.9
3,569,992 - -1.2
3,570,100 + Pf6N2E2_3082 0.49 -0.2
3,570,101 - Pf6N2E2_3082 0.50 -0.7
3,570,102 + Pf6N2E2_3082 0.51 -0.2
3,570,102 + Pf6N2E2_3082 0.51 -1.3
3,570,103 - Pf6N2E2_3082 0.51 -0.4
3,570,107 + Pf6N2E2_3082 0.53 -1.2
3,570,107 + Pf6N2E2_3082 0.53 +1.1
3,570,107 + Pf6N2E2_3082 0.53 -1.3
3,570,107 + Pf6N2E2_3082 0.53 -0.2
3,570,141 + Pf6N2E2_3082 0.72 -1.1
3,570,161 + Pf6N2E2_3082 0.83 -2.9
3,570,161 + Pf6N2E2_3082 0.83 -0.5

Or see this region's nucleotide sequence