Experiment: phosphite 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1868 and Pf6N2E2_1869 are separated by 822 nucleotides Pf6N2E2_1869 and Pf6N2E2_1870 are separated by 788 nucleotides
Pf6N2E2_1868 - hypothetical protein, at 2,213,819 to 2,214,892
_1868
Pf6N2E2_1869 - hypothetical protein, at 2,215,715 to 2,215,939
_1869
Pf6N2E2_1870 - hypothetical protein, at 2,216,728 to 2,217,258
_1870
Position (kb)
2215
2216 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2214.792 kb on + strand at 2214.793 kb on - strand at 2214.824 kb on + strand at 2214.927 kb on - strand at 2214.927 kb on - strand at 2214.980 kb on + strand at 2215.006 kb on - strand at 2215.012 kb on + strand at 2215.012 kb on + strand at 2215.012 kb on + strand at 2215.145 kb on + strand at 2215.146 kb on - strand at 2215.168 kb on + strand at 2215.169 kb on - strand at 2215.169 kb on - strand at 2215.169 kb on - strand at 2215.221 kb on + strand at 2215.240 kb on + strand at 2215.361 kb on + strand at 2215.388 kb on + strand at 2215.388 kb on + strand at 2215.388 kb on + strand at 2215.389 kb on - strand at 2215.394 kb on + strand at 2215.616 kb on + strand at 2215.616 kb on + strand at 2215.616 kb on + strand at 2215.616 kb on + strand at 2215.649 kb on + strand at 2215.650 kb on - strand at 2215.690 kb on - strand at 2215.788 kb on + strand, within Pf6N2E2_1869 at 2215.788 kb on + strand, within Pf6N2E2_1869 at 2215.789 kb on - strand, within Pf6N2E2_1869 at 2215.789 kb on - strand, within Pf6N2E2_1869 at 2215.824 kb on - strand, within Pf6N2E2_1869 at 2215.875 kb on + strand, within Pf6N2E2_1869 at 2215.875 kb on + strand, within Pf6N2E2_1869 at 2215.876 kb on - strand, within Pf6N2E2_1869 at 2215.876 kb on - strand, within Pf6N2E2_1869 at 2215.907 kb on + strand, within Pf6N2E2_1869 at 2215.916 kb on + strand, within Pf6N2E2_1869 at 2215.952 kb on + strand at 2216.002 kb on + strand at 2216.032 kb on + strand at 2216.032 kb on + strand at 2216.032 kb on + strand at 2216.044 kb on - strand at 2216.044 kb on - strand at 2216.086 kb on + strand at 2216.429 kb on + strand at 2216.430 kb on - strand at 2216.469 kb on + strand at 2216.470 kb on - strand at 2216.578 kb on + strand at 2216.578 kb on + strand at 2216.579 kb on - strand at 2216.579 kb on - strand at 2216.610 kb on - strand at 2216.683 kb on + strand at 2216.684 kb on - strand at 2216.684 kb on - strand at 2216.739 kb on + strand at 2216.740 kb on - strand at 2216.740 kb on - strand at 2216.740 kb on - strand at 2216.750 kb on - strand at 2216.750 kb on - strand at 2216.938 kb on + strand, within Pf6N2E2_1870
Per-strain Table
Position Strand Gene LocusTag Fraction phosphite 0.25 M remove 2,214,792 + +1.1 2,214,793 - +0.7 2,214,824 + -0.7 2,214,927 - -2.2 2,214,927 - -1.0 2,214,980 + +0.6 2,215,006 - +0.8 2,215,012 + +0.6 2,215,012 + +0.8 2,215,012 + -0.7 2,215,145 + -0.5 2,215,146 - +0.8 2,215,168 + -0.0 2,215,169 - -0.5 2,215,169 - +1.0 2,215,169 - -0.4 2,215,221 + -0.9 2,215,240 + +0.6 2,215,361 + +0.4 2,215,388 + +0.7 2,215,388 + -1.0 2,215,388 + +0.4 2,215,389 - -0.3 2,215,394 + +1.4 2,215,616 + +0.8 2,215,616 + +0.1 2,215,616 + -0.7 2,215,616 + -0.5 2,215,649 + -0.2 2,215,650 - -2.0 2,215,690 - -0.7 2,215,788 + Pf6N2E2_1869 0.32 +0.6 2,215,788 + Pf6N2E2_1869 0.32 -0.1 2,215,789 - Pf6N2E2_1869 0.33 -0.7 2,215,789 - Pf6N2E2_1869 0.33 -1.8 2,215,824 - Pf6N2E2_1869 0.48 -0.7 2,215,875 + Pf6N2E2_1869 0.71 -1.5 2,215,875 + Pf6N2E2_1869 0.71 -1.7 2,215,876 - Pf6N2E2_1869 0.72 -0.0 2,215,876 - Pf6N2E2_1869 0.72 +0.5 2,215,907 + Pf6N2E2_1869 0.85 +0.3 2,215,916 + Pf6N2E2_1869 0.89 -1.3 2,215,952 + -0.6 2,216,002 + -0.7 2,216,032 + +0.2 2,216,032 + +0.8 2,216,032 + +0.7 2,216,044 - -2.8 2,216,044 - -0.5 2,216,086 + -0.0 2,216,429 + +0.3 2,216,430 - +0.1 2,216,469 + +0.8 2,216,470 - +0.7 2,216,578 + -1.9 2,216,578 + -0.2 2,216,579 - -0.3 2,216,579 - +0.3 2,216,610 - +1.7 2,216,683 + +0.4 2,216,684 - +0.8 2,216,684 - +0.7 2,216,739 + -0.4 2,216,740 - +0.5 2,216,740 - -0.7 2,216,740 - +1.3 2,216,750 - +0.5 2,216,750 - -0.9 2,216,938 + Pf6N2E2_1870 0.40 -0.2
Or see this region's nucleotide sequence