Experiment: phosphite 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1767 and Pf6N2E2_1768 are separated by 57 nucleotides Pf6N2E2_1768 and Pf6N2E2_1769 overlap by 4 nucleotides Pf6N2E2_1769 and Pf6N2E2_1770 are separated by 44 nucleotides
Pf6N2E2_1767 - Transcriptional regulator, AraC family, at 2,088,739 to 2,089,500
_1767
Pf6N2E2_1768 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 2,089,558 to 2,090,982
_1768
Pf6N2E2_1769 - Nucleoside-diphosphate-sugar epimerases, at 2,090,979 to 2,091,320
_1769
Pf6N2E2_1770 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 2,091,365 to 2,092,438
_1770
Position (kb)
2089
2090
2091 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2088.582 kb on + strand at 2088.583 kb on - strand at 2088.583 kb on - strand at 2088.583 kb on - strand at 2088.601 kb on + strand at 2088.634 kb on + strand at 2088.812 kb on - strand at 2088.812 kb on - strand at 2088.880 kb on + strand, within Pf6N2E2_1767 at 2088.881 kb on - strand, within Pf6N2E2_1767 at 2088.916 kb on + strand, within Pf6N2E2_1767 at 2088.917 kb on - strand, within Pf6N2E2_1767 at 2088.917 kb on - strand, within Pf6N2E2_1767 at 2089.057 kb on - strand, within Pf6N2E2_1767 at 2089.057 kb on - strand, within Pf6N2E2_1767 at 2089.058 kb on + strand, within Pf6N2E2_1767 at 2089.067 kb on - strand, within Pf6N2E2_1767 at 2089.067 kb on - strand, within Pf6N2E2_1767 at 2089.067 kb on - strand, within Pf6N2E2_1767 at 2089.067 kb on - strand, within Pf6N2E2_1767 at 2089.125 kb on + strand, within Pf6N2E2_1767 at 2089.125 kb on + strand, within Pf6N2E2_1767 at 2089.126 kb on - strand, within Pf6N2E2_1767 at 2089.180 kb on + strand, within Pf6N2E2_1767 at 2089.249 kb on + strand, within Pf6N2E2_1767 at 2089.270 kb on + strand, within Pf6N2E2_1767 at 2089.270 kb on + strand, within Pf6N2E2_1767 at 2089.270 kb on + strand, within Pf6N2E2_1767 at 2089.303 kb on + strand, within Pf6N2E2_1767 at 2089.304 kb on - strand, within Pf6N2E2_1767 at 2089.304 kb on - strand, within Pf6N2E2_1767 at 2089.309 kb on + strand, within Pf6N2E2_1767 at 2089.423 kb on + strand, within Pf6N2E2_1767 at 2089.499 kb on - strand at 2089.630 kb on + strand at 2089.631 kb on - strand at 2089.693 kb on + strand at 2089.693 kb on + strand at 2089.696 kb on + strand at 2089.696 kb on + strand at 2089.696 kb on + strand at 2090.069 kb on + strand, within Pf6N2E2_1768 at 2090.069 kb on + strand, within Pf6N2E2_1768 at 2090.070 kb on - strand, within Pf6N2E2_1768 at 2090.099 kb on - strand, within Pf6N2E2_1768 at 2090.099 kb on - strand, within Pf6N2E2_1768 at 2090.175 kb on - strand, within Pf6N2E2_1768 at 2090.363 kb on + strand, within Pf6N2E2_1768 at 2090.364 kb on - strand, within Pf6N2E2_1768 at 2090.364 kb on - strand, within Pf6N2E2_1768 at 2090.364 kb on - strand, within Pf6N2E2_1768 at 2090.364 kb on - strand, within Pf6N2E2_1768 at 2090.364 kb on - strand, within Pf6N2E2_1768 at 2090.425 kb on + strand, within Pf6N2E2_1768 at 2090.425 kb on + strand, within Pf6N2E2_1768 at 2090.426 kb on - strand, within Pf6N2E2_1768 at 2090.476 kb on + strand, within Pf6N2E2_1768 at 2090.696 kb on + strand, within Pf6N2E2_1768 at 2090.696 kb on + strand, within Pf6N2E2_1768 at 2090.843 kb on - strand at 2090.883 kb on - strand at 2090.883 kb on - strand at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.224 kb on + strand, within Pf6N2E2_1769 at 2091.224 kb on + strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.280 kb on + strand, within Pf6N2E2_1769 at 2091.303 kb on + strand at 2091.304 kb on - strand at 2091.304 kb on - strand at 2091.304 kb on - strand at 2091.308 kb on - strand at 2091.388 kb on - strand at 2091.434 kb on + strand at 2091.434 kb on + strand at 2091.434 kb on + strand at 2091.449 kb on + strand at 2091.507 kb on - strand, within Pf6N2E2_1770 at 2091.507 kb on - strand, within Pf6N2E2_1770 at 2091.729 kb on - strand, within Pf6N2E2_1770 at 2091.729 kb on - strand, within Pf6N2E2_1770 at 2091.792 kb on + strand, within Pf6N2E2_1770 at 2091.792 kb on + strand, within Pf6N2E2_1770 at 2091.792 kb on + strand, within Pf6N2E2_1770 at 2091.793 kb on - strand, within Pf6N2E2_1770 at 2091.793 kb on - strand, within Pf6N2E2_1770 at 2091.905 kb on + strand, within Pf6N2E2_1770 at 2091.906 kb on - strand, within Pf6N2E2_1770 at 2091.906 kb on - strand, within Pf6N2E2_1770 at 2091.906 kb on - strand, within Pf6N2E2_1770 at 2091.945 kb on - strand, within Pf6N2E2_1770
Per-strain Table
Position Strand Gene LocusTag Fraction phosphite 0.25 M remove 2,088,582 + -0.5 2,088,583 - -1.5 2,088,583 - -0.1 2,088,583 - +1.3 2,088,601 + +0.7 2,088,634 + -2.1 2,088,812 - +0.7 2,088,812 - +1.9 2,088,880 + Pf6N2E2_1767 0.19 -1.6 2,088,881 - Pf6N2E2_1767 0.19 +0.3 2,088,916 + Pf6N2E2_1767 0.23 +1.3 2,088,917 - Pf6N2E2_1767 0.23 -1.7 2,088,917 - Pf6N2E2_1767 0.23 +1.3 2,089,057 - Pf6N2E2_1767 0.42 -0.9 2,089,057 - Pf6N2E2_1767 0.42 -0.1 2,089,058 + Pf6N2E2_1767 0.42 +0.1 2,089,067 - Pf6N2E2_1767 0.43 -0.5 2,089,067 - Pf6N2E2_1767 0.43 +0.1 2,089,067 - Pf6N2E2_1767 0.43 -1.3 2,089,067 - Pf6N2E2_1767 0.43 +0.3 2,089,125 + Pf6N2E2_1767 0.51 +0.3 2,089,125 + Pf6N2E2_1767 0.51 -0.9 2,089,126 - Pf6N2E2_1767 0.51 -0.6 2,089,180 + Pf6N2E2_1767 0.58 -0.5 2,089,249 + Pf6N2E2_1767 0.67 +0.2 2,089,270 + Pf6N2E2_1767 0.70 -0.7 2,089,270 + Pf6N2E2_1767 0.70 +0.4 2,089,270 + Pf6N2E2_1767 0.70 +1.7 2,089,303 + Pf6N2E2_1767 0.74 -0.5 2,089,304 - Pf6N2E2_1767 0.74 -0.5 2,089,304 - Pf6N2E2_1767 0.74 +0.4 2,089,309 + Pf6N2E2_1767 0.75 +0.7 2,089,423 + Pf6N2E2_1767 0.90 -0.7 2,089,499 - +0.9 2,089,630 + +1.1 2,089,631 - -1.3 2,089,693 + +0.6 2,089,693 + -0.7 2,089,696 + +0.7 2,089,696 + +0.3 2,089,696 + -0.5 2,090,069 + Pf6N2E2_1768 0.36 -0.3 2,090,069 + Pf6N2E2_1768 0.36 +0.6 2,090,070 - Pf6N2E2_1768 0.36 +0.8 2,090,099 - Pf6N2E2_1768 0.38 -0.7 2,090,099 - Pf6N2E2_1768 0.38 +0.7 2,090,175 - Pf6N2E2_1768 0.43 +0.3 2,090,363 + Pf6N2E2_1768 0.56 +0.1 2,090,364 - Pf6N2E2_1768 0.57 -0.6 2,090,364 - Pf6N2E2_1768 0.57 +0.8 2,090,364 - Pf6N2E2_1768 0.57 +2.3 2,090,364 - Pf6N2E2_1768 0.57 +0.1 2,090,364 - Pf6N2E2_1768 0.57 +1.7 2,090,425 + Pf6N2E2_1768 0.61 -0.2 2,090,425 + Pf6N2E2_1768 0.61 +0.5 2,090,426 - Pf6N2E2_1768 0.61 +0.8 2,090,476 + Pf6N2E2_1768 0.64 -0.5 2,090,696 + Pf6N2E2_1768 0.80 -1.2 2,090,696 + Pf6N2E2_1768 0.80 -0.1 2,090,843 - -0.6 2,090,883 - -0.5 2,090,883 - -0.0 2,091,220 + Pf6N2E2_1769 0.70 -0.5 2,091,220 + Pf6N2E2_1769 0.70 -0.7 2,091,220 + Pf6N2E2_1769 0.70 -0.2 2,091,220 + Pf6N2E2_1769 0.70 +0.1 2,091,220 + Pf6N2E2_1769 0.70 +2.0 2,091,220 + Pf6N2E2_1769 0.70 -0.1 2,091,221 - Pf6N2E2_1769 0.71 -0.3 2,091,221 - Pf6N2E2_1769 0.71 +0.1 2,091,221 - Pf6N2E2_1769 0.71 +0.8 2,091,221 - Pf6N2E2_1769 0.71 +1.0 2,091,224 + Pf6N2E2_1769 0.72 +0.3 2,091,224 + Pf6N2E2_1769 0.72 -0.9 2,091,225 - Pf6N2E2_1769 0.72 +1.4 2,091,225 - Pf6N2E2_1769 0.72 -0.4 2,091,225 - Pf6N2E2_1769 0.72 +0.4 2,091,225 - Pf6N2E2_1769 0.72 -1.9 2,091,280 + Pf6N2E2_1769 0.88 -0.5 2,091,303 + +0.3 2,091,304 - +0.5 2,091,304 - +1.0 2,091,304 - +0.6 2,091,308 - +3.1 2,091,388 - +0.8 2,091,434 + -0.5 2,091,434 + -1.1 2,091,434 + +0.8 2,091,449 + +0.7 2,091,507 - Pf6N2E2_1770 0.13 -0.9 2,091,507 - Pf6N2E2_1770 0.13 -0.2 2,091,729 - Pf6N2E2_1770 0.34 +1.1 2,091,729 - Pf6N2E2_1770 0.34 +1.3 2,091,792 + Pf6N2E2_1770 0.40 +1.3 2,091,792 + Pf6N2E2_1770 0.40 -0.0 2,091,792 + Pf6N2E2_1770 0.40 +1.3 2,091,793 - Pf6N2E2_1770 0.40 -0.6 2,091,793 - Pf6N2E2_1770 0.40 -0.7 2,091,905 + Pf6N2E2_1770 0.50 -0.2 2,091,906 - Pf6N2E2_1770 0.50 -0.6 2,091,906 - Pf6N2E2_1770 0.50 -0.7 2,091,906 - Pf6N2E2_1770 0.50 -0.0 2,091,945 - Pf6N2E2_1770 0.54 +0.1
Or see this region's nucleotide sequence