Experiment: phosphite 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1490 and Pf6N2E2_1491 are separated by 114 nucleotides Pf6N2E2_1491 and Pf6N2E2_1492 are separated by 45 nucleotides Pf6N2E2_1492 and Pf6N2E2_1493 are separated by 27 nucleotides
Pf6N2E2_1490 - hypothetical protein, at 1,743,433 to 1,743,597
_1490
Pf6N2E2_1491 - Cystathionine beta-lyase (EC 4.4.1.8), at 1,743,712 to 1,746,477
_1491
Pf6N2E2_1492 - No significant database matches, at 1,746,523 to 1,747,122
_1492
Pf6N2E2_1493 - ABC-type polar amino acid transport system, ATPase component, at 1,747,150 to 1,747,986
_1493
Position (kb)
1743
1744
1745
1746
1747 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1742.804 kb on - strand at 1742.872 kb on + strand at 1742.875 kb on + strand at 1742.956 kb on + strand at 1743.080 kb on - strand at 1743.392 kb on - strand at 1743.405 kb on + strand at 1743.473 kb on - strand, within Pf6N2E2_1490 at 1743.554 kb on + strand, within Pf6N2E2_1490 at 1743.559 kb on - strand, within Pf6N2E2_1490 at 1743.620 kb on + strand at 1743.938 kb on + strand at 1743.958 kb on + strand at 1744.155 kb on - strand, within Pf6N2E2_1491 at 1744.271 kb on + strand, within Pf6N2E2_1491 at 1744.272 kb on - strand, within Pf6N2E2_1491 at 1744.344 kb on + strand, within Pf6N2E2_1491 at 1744.345 kb on - strand, within Pf6N2E2_1491 at 1744.345 kb on - strand, within Pf6N2E2_1491 at 1744.513 kb on + strand, within Pf6N2E2_1491 at 1744.563 kb on - strand, within Pf6N2E2_1491 at 1744.563 kb on - strand, within Pf6N2E2_1491 at 1744.605 kb on - strand, within Pf6N2E2_1491 at 1744.727 kb on + strand, within Pf6N2E2_1491 at 1744.728 kb on - strand, within Pf6N2E2_1491 at 1744.985 kb on + strand, within Pf6N2E2_1491 at 1744.986 kb on - strand, within Pf6N2E2_1491 at 1745.431 kb on + strand, within Pf6N2E2_1491 at 1745.431 kb on + strand, within Pf6N2E2_1491 at 1745.431 kb on + strand, within Pf6N2E2_1491 at 1745.431 kb on + strand, within Pf6N2E2_1491 at 1745.432 kb on - strand, within Pf6N2E2_1491 at 1745.534 kb on + strand, within Pf6N2E2_1491 at 1745.534 kb on + strand, within Pf6N2E2_1491 at 1745.534 kb on + strand, within Pf6N2E2_1491 at 1745.535 kb on - strand, within Pf6N2E2_1491 at 1745.535 kb on - strand, within Pf6N2E2_1491 at 1745.840 kb on + strand, within Pf6N2E2_1491 at 1745.840 kb on + strand, within Pf6N2E2_1491 at 1745.840 kb on + strand, within Pf6N2E2_1491 at 1745.840 kb on + strand, within Pf6N2E2_1491 at 1745.841 kb on - strand, within Pf6N2E2_1491 at 1745.841 kb on - strand, within Pf6N2E2_1491 at 1745.841 kb on - strand, within Pf6N2E2_1491 at 1746.118 kb on + strand, within Pf6N2E2_1491 at 1746.119 kb on - strand, within Pf6N2E2_1491 at 1746.119 kb on - strand, within Pf6N2E2_1491 at 1746.346 kb on - strand at 1746.463 kb on + strand at 1746.464 kb on - strand at 1746.534 kb on - strand at 1746.569 kb on + strand at 1746.570 kb on - strand at 1746.636 kb on - strand, within Pf6N2E2_1492 at 1746.715 kb on - strand, within Pf6N2E2_1492 at 1746.715 kb on - strand, within Pf6N2E2_1492 at 1746.758 kb on + strand, within Pf6N2E2_1492 at 1746.792 kb on - strand, within Pf6N2E2_1492 at 1747.117 kb on - strand at 1747.191 kb on - strand at 1747.191 kb on - strand at 1747.191 kb on - strand at 1747.243 kb on + strand, within Pf6N2E2_1493 at 1747.332 kb on - strand, within Pf6N2E2_1493 at 1747.382 kb on - strand, within Pf6N2E2_1493
Per-strain Table
Position Strand Gene LocusTag Fraction phosphite 0.25 M remove 1,742,804 - -0.0 1,742,872 + +0.4 1,742,875 + -0.0 1,742,956 + +0.6 1,743,080 - +1.1 1,743,392 - +1.9 1,743,405 + +0.5 1,743,473 - Pf6N2E2_1490 0.24 -0.4 1,743,554 + Pf6N2E2_1490 0.73 -0.6 1,743,559 - Pf6N2E2_1490 0.76 +0.1 1,743,620 + -0.1 1,743,938 + +1.1 1,743,958 + +0.1 1,744,155 - Pf6N2E2_1491 0.16 +0.3 1,744,271 + Pf6N2E2_1491 0.20 +1.1 1,744,272 - Pf6N2E2_1491 0.20 -0.0 1,744,344 + Pf6N2E2_1491 0.23 +0.9 1,744,345 - Pf6N2E2_1491 0.23 +0.5 1,744,345 - Pf6N2E2_1491 0.23 -0.4 1,744,513 + Pf6N2E2_1491 0.29 +0.4 1,744,563 - Pf6N2E2_1491 0.31 +0.5 1,744,563 - Pf6N2E2_1491 0.31 -1.4 1,744,605 - Pf6N2E2_1491 0.32 +0.0 1,744,727 + Pf6N2E2_1491 0.37 -0.4 1,744,728 - Pf6N2E2_1491 0.37 -1.4 1,744,985 + Pf6N2E2_1491 0.46 -0.1 1,744,986 - Pf6N2E2_1491 0.46 -0.4 1,745,431 + Pf6N2E2_1491 0.62 +1.3 1,745,431 + Pf6N2E2_1491 0.62 +0.5 1,745,431 + Pf6N2E2_1491 0.62 +0.1 1,745,431 + Pf6N2E2_1491 0.62 -1.2 1,745,432 - Pf6N2E2_1491 0.62 +1.0 1,745,534 + Pf6N2E2_1491 0.66 +0.4 1,745,534 + Pf6N2E2_1491 0.66 +0.1 1,745,534 + Pf6N2E2_1491 0.66 +1.0 1,745,535 - Pf6N2E2_1491 0.66 +1.0 1,745,535 - Pf6N2E2_1491 0.66 -1.9 1,745,840 + Pf6N2E2_1491 0.77 -1.7 1,745,840 + Pf6N2E2_1491 0.77 +1.0 1,745,840 + Pf6N2E2_1491 0.77 +0.6 1,745,840 + Pf6N2E2_1491 0.77 +0.1 1,745,841 - Pf6N2E2_1491 0.77 +0.4 1,745,841 - Pf6N2E2_1491 0.77 +1.3 1,745,841 - Pf6N2E2_1491 0.77 -1.2 1,746,118 + Pf6N2E2_1491 0.87 +0.8 1,746,119 - Pf6N2E2_1491 0.87 +0.7 1,746,119 - Pf6N2E2_1491 0.87 +0.2 1,746,346 - +0.6 1,746,463 + -0.2 1,746,464 - -1.0 1,746,534 - -0.6 1,746,569 + -1.9 1,746,570 - +0.4 1,746,636 - Pf6N2E2_1492 0.19 -1.2 1,746,715 - Pf6N2E2_1492 0.32 -0.7 1,746,715 - Pf6N2E2_1492 0.32 -0.4 1,746,758 + Pf6N2E2_1492 0.39 -0.2 1,746,792 - Pf6N2E2_1492 0.45 -1.9 1,747,117 - -0.6 1,747,191 - +0.3 1,747,191 - +0.4 1,747,191 - +0.8 1,747,243 + Pf6N2E2_1493 0.11 -0.1 1,747,332 - Pf6N2E2_1493 0.22 -0.2 1,747,382 - Pf6N2E2_1493 0.28 -1.9
Or see this region's nucleotide sequence