Experiment: Hydroxy-L-Proline (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06556 and H281DRAFT_06557 are separated by 147 nucleotides H281DRAFT_06557 and H281DRAFT_06558 are separated by 78 nucleotides H281DRAFT_06558 and H281DRAFT_06559 overlap by 18 nucleotides H281DRAFT_06559 and H281DRAFT_06560 are separated by 85 nucleotides H281DRAFT_06560 and H281DRAFT_06561 overlap by 10 nucleotides H281DRAFT_06561 and H281DRAFT_06562 are separated by 90 nucleotides H281DRAFT_06562 and H281DRAFT_06563 are separated by 236 nucleotides
H281DRAFT_06556: H281DRAFT_06556 - hypothetical protein, at 758 to 979
_06556
H281DRAFT_06557: H281DRAFT_06557 - hypothetical protein, at 1,127 to 1,321
_06557
H281DRAFT_06558: H281DRAFT_06558 - hypothetical protein, at 1,400 to 1,603
_06558
H281DRAFT_06559: H281DRAFT_06559 - hypothetical protein, at 1,586 to 1,891
_06559
H281DRAFT_06560: H281DRAFT_06560 - hypothetical protein, at 1,977 to 2,675
_06560
H281DRAFT_06561: H281DRAFT_06561 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 2,666 to 3,037
_06561
H281DRAFT_06562: H281DRAFT_06562 - hypothetical protein, at 3,128 to 3,346
_06562
H281DRAFT_06563: H281DRAFT_06563 - hypothetical protein, at 3,583 to 3,846
_06563
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-2
-1
0
1 at 1.014 kb on - strand at 1.043 kb on + strand at 1.060 kb on + strand at 1.256 kb on + strand, within H281DRAFT_06557 at 1.319 kb on + strand at 1.349 kb on - strand at 1.358 kb on + strand at 1.497 kb on + strand, within H281DRAFT_06558 at 1.497 kb on + strand, within H281DRAFT_06558 at 1.498 kb on - strand, within H281DRAFT_06558 at 1.524 kb on - strand, within H281DRAFT_06558 at 1.556 kb on + strand, within H281DRAFT_06558 at 1.557 kb on - strand, within H281DRAFT_06558 at 1.575 kb on + strand, within H281DRAFT_06558 at 1.575 kb on + strand, within H281DRAFT_06558 at 1.584 kb on + strand at 1.584 kb on + strand at 1.585 kb on - strand at 1.585 kb on - strand at 1.587 kb on + strand at 1.587 kb on + strand at 1.995 kb on - strand at 2.147 kb on - strand, within H281DRAFT_06560 at 2.315 kb on - strand, within H281DRAFT_06560 at 2.376 kb on + strand, within H281DRAFT_06560 at 2.613 kb on + strand at 2.613 kb on + strand at 2.613 kb on + strand at 2.614 kb on - strand at 2.642 kb on + strand at 2.674 kb on - strand at 2.708 kb on + strand, within H281DRAFT_06561 at 2.709 kb on - strand, within H281DRAFT_06561 at 2.787 kb on + strand, within H281DRAFT_06561 at 2.788 kb on - strand, within H281DRAFT_06561 at 2.788 kb on - strand, within H281DRAFT_06561 at 2.788 kb on - strand, within H281DRAFT_06561 at 2.788 kb on - strand, within H281DRAFT_06561 at 2.891 kb on + strand, within H281DRAFT_06561 at 2.891 kb on + strand, within H281DRAFT_06561 at 2.892 kb on - strand, within H281DRAFT_06561 at 2.917 kb on + strand, within H281DRAFT_06561 at 2.918 kb on - strand at 2.918 kb on - strand, within H281DRAFT_06561 at 2.918 kb on - strand, within H281DRAFT_06561 at 2.923 kb on + strand, within H281DRAFT_06561 at 2.924 kb on - strand, within H281DRAFT_06561 at 3.012 kb on + strand at 3.012 kb on + strand at 3.012 kb on + strand at 3.013 kb on - strand at 3.013 kb on - strand at 3.013 kb on - strand at 3.013 kb on - strand at 3.095 kb on + strand at 3.095 kb on + strand at 3.141 kb on + strand at 3.142 kb on - strand at 3.250 kb on + strand, within H281DRAFT_06562 at 3.334 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.347 kb on - strand at 3.347 kb on - strand at 3.347 kb on - strand at 3.347 kb on - strand at 3.347 kb on - strand at 3.348 kb on + strand at 3.349 kb on - strand at 3.349 kb on - strand at 3.356 kb on + strand at 3.357 kb on - strand at 3.357 kb on - strand at 3.357 kb on - strand at 3.375 kb on + strand at 3.375 kb on + strand at 3.376 kb on - strand at 3.376 kb on - strand at 3.376 kb on - strand at 3.430 kb on - strand at 3.435 kb on + strand at 3.436 kb on - strand at 3.558 kb on + strand at 3.559 kb on - strand at 3.563 kb on + strand at 3.563 kb on + strand at 3.568 kb on + strand at 3.568 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydroxy-L-Proline (C) remove 1,014 - +0.3 1,043 + +0.7 1,060 + -0.4 1,256 + H281DRAFT_06557 0.66 +0.2 1,319 + +0.8 1,349 - -0.2 1,358 + +0.1 1,497 + H281DRAFT_06558 0.48 +0.5 1,497 + H281DRAFT_06558 0.48 +0.1 1,498 - H281DRAFT_06558 0.48 -1.7 1,524 - H281DRAFT_06558 0.61 -0.5 1,556 + H281DRAFT_06558 0.76 -0.5 1,557 - H281DRAFT_06558 0.77 +0.0 1,575 + H281DRAFT_06558 0.86 -0.1 1,575 + H281DRAFT_06558 0.86 +0.1 1,584 + -1.5 1,584 + -0.7 1,585 - -0.6 1,585 - +0.0 1,587 + -1.7 1,587 + +0.2 1,995 - +0.3 2,147 - H281DRAFT_06560 0.24 +0.4 2,315 - H281DRAFT_06560 0.48 -0.1 2,376 + H281DRAFT_06560 0.57 -0.3 2,613 + -0.4 2,613 + -0.4 2,613 + +0.4 2,614 - -0.3 2,642 + +0.1 2,674 - +0.3 2,708 + H281DRAFT_06561 0.11 -0.1 2,709 - H281DRAFT_06561 0.12 -0.0 2,787 + H281DRAFT_06561 0.33 -0.2 2,788 - H281DRAFT_06561 0.33 -0.6 2,788 - H281DRAFT_06561 0.33 +1.0 2,788 - H281DRAFT_06561 0.33 +0.5 2,788 - H281DRAFT_06561 0.33 -0.8 2,891 + H281DRAFT_06561 0.60 -1.0 2,891 + H281DRAFT_06561 0.60 -0.3 2,892 - H281DRAFT_06561 0.61 +0.4 2,917 + H281DRAFT_06561 0.67 +0.2 2,918 - +0.5 2,918 - H281DRAFT_06561 0.68 -0.3 2,918 - H281DRAFT_06561 0.68 -0.3 2,923 + H281DRAFT_06561 0.69 +0.2 2,924 - H281DRAFT_06561 0.69 -0.2 3,012 + +0.7 3,012 + +0.2 3,012 + -0.5 3,013 - +0.1 3,013 - +0.3 3,013 - -0.2 3,013 - +1.7 3,095 + -1.7 3,095 + +0.1 3,141 + +0.1 3,142 - -0.0 3,250 + H281DRAFT_06562 0.56 +1.0 3,334 + -0.3 3,346 + -1.2 3,346 + -0.3 3,346 + -1.5 3,346 + -0.1 3,346 + -0.3 3,346 + +0.0 3,346 + +0.8 3,346 + +0.0 3,347 - -0.2 3,347 - +1.3 3,347 - -1.5 3,347 - +0.1 3,347 - -0.1 3,348 + +0.0 3,349 - -0.2 3,349 - -0.3 3,356 + +0.9 3,357 - +0.0 3,357 - -1.2 3,357 - -0.4 3,375 + +0.2 3,375 + +0.3 3,376 - -2.0 3,376 - -0.6 3,376 - -2.2 3,430 - -0.4 3,435 + -0.4 3,436 - -0.3 3,558 + +0.1 3,559 - +0.1 3,563 + +0.6 3,563 + -0.1 3,568 + -0.2 3,568 + +0.3
Or see this region's nucleotide sequence