Experiment: L-Phenylalanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2343 and HP15_tRNA44 are separated by 53 nucleotides HP15_tRNA44 and HP15_2344 are separated by 99 nucleotides HP15_2344 and HP15_2345 are separated by 316 nucleotides
HP15_2343: HP15_2343 - amidase signature enzyme, at 2,465,028 to 2,466,515
_2343
HP15_tRNA44: HP15_tRNA44 - tRNA-Met, at 2,466,569 to 2,466,645
_tRNA44
HP15_2344: HP15_2344 - acetyl-CoA acetyltransferase, at 2,466,745 to 2,468,088
_2344
HP15_2345: HP15_2345 - 3-oxoacyl-(acyl-carrier protein) reductase, at 2,468,405 to 2,469,811
_2345
Position (kb)
2466
2467
2468
2469 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2465.800 kb on + strand, within HP15_2343 at 2465.805 kb on + strand, within HP15_2343 at 2465.805 kb on + strand, within HP15_2343 at 2465.887 kb on - strand, within HP15_2343 at 2465.921 kb on + strand, within HP15_2343 at 2465.967 kb on - strand, within HP15_2343 at 2465.969 kb on + strand, within HP15_2343 at 2466.005 kb on + strand, within HP15_2343 at 2466.005 kb on + strand, within HP15_2343 at 2466.013 kb on + strand, within HP15_2343 at 2466.013 kb on - strand, within HP15_2343 at 2466.013 kb on - strand, within HP15_2343 at 2466.302 kb on + strand, within HP15_2343 at 2466.310 kb on - strand, within HP15_2343 at 2466.318 kb on + strand, within HP15_2343 at 2466.440 kb on - strand at 2466.465 kb on + strand at 2466.509 kb on - strand at 2466.633 kb on + strand, within HP15_tRNA44 at 2466.641 kb on - strand at 2466.641 kb on - strand at 2466.641 kb on - strand at 2466.670 kb on + strand at 2466.713 kb on - strand at 2466.921 kb on + strand, within HP15_2344 at 2466.931 kb on + strand, within HP15_2344 at 2466.943 kb on + strand, within HP15_2344 at 2466.970 kb on + strand, within HP15_2344 at 2466.978 kb on + strand, within HP15_2344 at 2466.980 kb on + strand, within HP15_2344 at 2466.995 kb on - strand, within HP15_2344 at 2466.997 kb on + strand, within HP15_2344 at 2466.997 kb on + strand, within HP15_2344 at 2467.091 kb on - strand, within HP15_2344 at 2467.096 kb on - strand, within HP15_2344 at 2467.165 kb on + strand, within HP15_2344 at 2467.384 kb on + strand, within HP15_2344 at 2467.435 kb on + strand, within HP15_2344 at 2467.472 kb on - strand, within HP15_2344 at 2467.487 kb on - strand, within HP15_2344 at 2467.535 kb on + strand, within HP15_2344 at 2467.589 kb on + strand, within HP15_2344 at 2467.589 kb on + strand, within HP15_2344 at 2467.600 kb on + strand, within HP15_2344 at 2467.608 kb on - strand, within HP15_2344 at 2467.695 kb on + strand, within HP15_2344 at 2467.753 kb on - strand, within HP15_2344 at 2467.788 kb on + strand, within HP15_2344 at 2467.796 kb on - strand, within HP15_2344 at 2467.879 kb on + strand, within HP15_2344 at 2467.911 kb on + strand, within HP15_2344 at 2467.963 kb on - strand at 2467.963 kb on - strand at 2468.073 kb on + strand at 2468.156 kb on + strand at 2468.238 kb on - strand at 2468.296 kb on - strand at 2468.353 kb on - strand at 2468.414 kb on - strand at 2468.414 kb on - strand at 2468.635 kb on + strand, within HP15_2345 at 2468.753 kb on + strand, within HP15_2345 at 2468.760 kb on - strand, within HP15_2345 at 2468.875 kb on + strand, within HP15_2345 at 2468.875 kb on - strand, within HP15_2345 at 2468.992 kb on - strand, within HP15_2345 at 2469.019 kb on - strand, within HP15_2345
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine remove 2,465,800 + HP15_2343 0.52 +1.7 2,465,805 + HP15_2343 0.52 -0.2 2,465,805 + HP15_2343 0.52 -0.7 2,465,887 - HP15_2343 0.58 -0.1 2,465,921 + HP15_2343 0.60 -0.1 2,465,967 - HP15_2343 0.63 +1.3 2,465,969 + HP15_2343 0.63 -0.9 2,466,005 + HP15_2343 0.66 -1.5 2,466,005 + HP15_2343 0.66 +1.8 2,466,013 + HP15_2343 0.66 -0.7 2,466,013 - HP15_2343 0.66 +0.4 2,466,013 - HP15_2343 0.66 +0.1 2,466,302 + HP15_2343 0.86 +1.4 2,466,310 - HP15_2343 0.86 -0.0 2,466,318 + HP15_2343 0.87 +0.0 2,466,440 - -0.2 2,466,465 + +1.2 2,466,509 - -0.6 2,466,633 + HP15_tRNA44 0.83 -0.2 2,466,641 - +1.4 2,466,641 - -1.0 2,466,641 - -0.6 2,466,670 + -1.7 2,466,713 - +1.3 2,466,921 + HP15_2344 0.13 +1.1 2,466,931 + HP15_2344 0.14 +1.6 2,466,943 + HP15_2344 0.15 -0.4 2,466,970 + HP15_2344 0.17 -0.4 2,466,978 + HP15_2344 0.17 +1.7 2,466,980 + HP15_2344 0.17 +0.3 2,466,995 - HP15_2344 0.19 +0.8 2,466,997 + HP15_2344 0.19 +0.2 2,466,997 + HP15_2344 0.19 +0.1 2,467,091 - HP15_2344 0.26 +1.4 2,467,096 - HP15_2344 0.26 +1.8 2,467,165 + HP15_2344 0.31 -0.4 2,467,384 + HP15_2344 0.48 +0.2 2,467,435 + HP15_2344 0.51 +0.8 2,467,472 - HP15_2344 0.54 +1.3 2,467,487 - HP15_2344 0.55 +0.3 2,467,535 + HP15_2344 0.59 +1.9 2,467,589 + HP15_2344 0.63 -1.2 2,467,589 + HP15_2344 0.63 -0.8 2,467,600 + HP15_2344 0.64 -1.0 2,467,608 - HP15_2344 0.64 +0.1 2,467,695 + HP15_2344 0.71 -0.4 2,467,753 - HP15_2344 0.75 +1.7 2,467,788 + HP15_2344 0.78 -0.1 2,467,796 - HP15_2344 0.78 +1.1 2,467,879 + HP15_2344 0.84 -0.2 2,467,911 + HP15_2344 0.87 +1.2 2,467,963 - -0.7 2,467,963 - +0.7 2,468,073 + -0.1 2,468,156 + +1.2 2,468,238 - -3.0 2,468,296 - -0.5 2,468,353 - +1.3 2,468,414 - -0.8 2,468,414 - -1.8 2,468,635 + HP15_2345 0.16 +0.4 2,468,753 + HP15_2345 0.25 +1.5 2,468,760 - HP15_2345 0.25 +0.8 2,468,875 + HP15_2345 0.33 -0.8 2,468,875 - HP15_2345 0.33 -0.3 2,468,992 - HP15_2345 0.42 +0.1 2,469,019 - HP15_2345 0.44 -0.4
Or see this region's nucleotide sequence