Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08220

Experiment: N-Acetyl-D-glucosamine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08215 and BWI76_RS08220 are separated by 312 nucleotidesBWI76_RS08220 and BWI76_RS08225 are separated by 178 nucleotides BWI76_RS08215: BWI76_RS08215 - glucosamine-6-phosphate deaminase, at 1,737,921 to 1,738,721 _RS08215 BWI76_RS08220: BWI76_RS08220 - PTS N-acetyl glucosamine transporter subunit IIABC, at 1,739,034 to 1,740,986 _RS08220 BWI76_RS08225: BWI76_RS08225 - glutamine--tRNA ligase, at 1,741,165 to 1,742,832 _RS08225 Position (kb) 1739 1740 1741Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 1738.242 kb on + strand, within BWI76_RS08215at 1738.483 kb on + strand, within BWI76_RS08215at 1738.505 kb on + strand, within BWI76_RS08215at 1738.534 kb on + strand, within BWI76_RS08215at 1738.696 kb on + strandat 1738.773 kb on + strandat 1739.234 kb on - strandat 1739.299 kb on + strand, within BWI76_RS08220at 1739.595 kb on + strand, within BWI76_RS08220at 1739.715 kb on - strand, within BWI76_RS08220at 1739.815 kb on + strand, within BWI76_RS08220at 1739.880 kb on - strand, within BWI76_RS08220at 1739.932 kb on + strand, within BWI76_RS08220at 1740.090 kb on + strand, within BWI76_RS08220at 1740.095 kb on + strand, within BWI76_RS08220at 1740.316 kb on - strand, within BWI76_RS08220at 1740.823 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N-Acetyl-D-glucosamine (N)
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1,738,242 + BWI76_RS08215 0.40 -0.3
1,738,483 + BWI76_RS08215 0.70 -2.6
1,738,505 + BWI76_RS08215 0.73 -5.1
1,738,534 + BWI76_RS08215 0.77 -3.5
1,738,696 + -4.8
1,738,773 + -2.7
1,739,234 - -0.5
1,739,299 + BWI76_RS08220 0.14 -4.1
1,739,595 + BWI76_RS08220 0.29 -4.3
1,739,715 - BWI76_RS08220 0.35 -1.1
1,739,815 + BWI76_RS08220 0.40 -3.0
1,739,880 - BWI76_RS08220 0.43 -3.8
1,739,932 + BWI76_RS08220 0.46 -2.0
1,740,090 + BWI76_RS08220 0.54 -2.2
1,740,095 + BWI76_RS08220 0.54 -7.2
1,740,316 - BWI76_RS08220 0.66 -2.6
1,740,823 + -3.8

Or see this region's nucleotide sequence