Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS19030

Experiment: reconstituted MRS with Sialyllacto-N-tetraose a 5.0 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	reconstituted MRS with Sialyllacto-N-tetraose a 5.0 mM
2,046,576	+		BBR_RS19025	0.41	-1.7
2,046,576	+		BBR_RS19025	0.41	+0.1
2,046,581	+		BBR_RS19025	0.41	-0.5
2,046,584	-		BBR_RS19025	0.41	-0.7
2,046,591	+		BBR_RS19025	0.42	-0.1
2,046,593	-		BBR_RS19025	0.42	+0.0
2,046,601	-		BBR_RS19025	0.43	-0.4
2,046,603	+		BBR_RS19025	0.43	-0.0
2,046,605	+		BBR_RS19025	0.43	+0.6
2,046,621	-		BBR_RS19025	0.44	+1.0
2,046,632	-		BBR_RS19025	0.45	+0.1
2,046,633	-		BBR_RS19025	0.45	+0.8
2,046,657	-		BBR_RS19025	0.47	+0.2
2,046,657	-		BBR_RS19025	0.47	-0.8
2,046,658	+		BBR_RS19025	0.47	+0.3
2,046,687	+		BBR_RS19025	0.49	-0.1
2,046,690	-		BBR_RS19025	0.49	+0.7
2,046,695	-		BBR_RS19025	0.50	-0.3
2,046,731	-		BBR_RS19025	0.53	+0.3
2,046,733	+		BBR_RS19025	0.53	+0.4
2,046,773	-		BBR_RS19025	0.56	-1.8
2,046,792	-		BBR_RS19025	0.57	-1.3
2,046,792	-		BBR_RS19025	0.57	+1.1
2,046,799	-		BBR_RS19025	0.58	-0.5
2,046,799	-		BBR_RS19025	0.58	+0.0
2,046,803	+		BBR_RS19025	0.58	+0.5
2,046,813	+		BBR_RS19025	0.59	-0.2
2,046,836	-		BBR_RS19025	0.61	+0.9
2,046,840	+		BBR_RS19025	0.61	+0.7
2,046,840	+		BBR_RS19025	0.61	+0.6
2,046,863	+		BBR_RS19025	0.63	-0.9
2,046,866	+		BBR_RS19025	0.63	-0.1
2,046,866	+		BBR_RS19025	0.63	-0.6
2,046,866	+		BBR_RS19025	0.63	+0.1
2,046,866	+		BBR_RS19025	0.63	+0.2
2,046,866	+		BBR_RS19025	0.63	+0.3
2,046,868	+		BBR_RS19025	0.63	+0.7
2,046,876	+		BBR_RS19025	0.64	-0.5
2,046,876	-		BBR_RS19025	0.64	-0.6
2,046,876	-		BBR_RS19025	0.64	-0.3
2,046,876	-		BBR_RS19025	0.64	-0.0
2,046,876	-		BBR_RS19025	0.64	+0.1
2,046,879	-		BBR_RS19025	0.64	-0.1
2,046,883	+		BBR_RS19025	0.64	+0.5
2,046,883	+		BBR_RS19025	0.64	-0.1
2,046,883	+		BBR_RS19025	0.64	-0.2
2,046,883	+		BBR_RS19025	0.64	-0.1
2,046,888	+		BBR_RS19025	0.65	+0.8
2,046,891	-		BBR_RS19025	0.65	+0.7
2,046,891	-		BBR_RS19025	0.65	+0.8
2,046,891	-		BBR_RS19025	0.65	-1.7
2,046,891	-		BBR_RS19025	0.65	+2.3
2,046,906	-		BBR_RS19025	0.66	-0.7
2,046,926	+		BBR_RS19025	0.68	+0.9
2,046,971	-		BBR_RS19025	0.71	-2.1
2,047,023	-		BBR_RS19025	0.75	+0.4
2,047,032	-		BBR_RS19025	0.76	-0.1
2,047,032	-		BBR_RS19025	0.76	-0.5
2,047,032	-		BBR_RS19025	0.76	-0.5
2,047,035	+		BBR_RS19025	0.76	+0.6
2,047,035	+		BBR_RS19025	0.76	+0.3
2,047,066	-		BBR_RS19025	0.78	+0.4
2,047,088	+		BBR_RS19025	0.80	-0.2
2,047,096	-		BBR_RS19025	0.81	-0.4
2,047,096	-		BBR_RS19025	0.81	+0.7
2,047,103	-		BBR_RS19025	0.81	+0.5
2,047,103	-		BBR_RS19025	0.81	+0.5
2,047,107	+		BBR_RS19025	0.82	-0.9
2,047,108	-		BBR_RS19025	0.82	-0.0
2,047,115	+		BBR_RS19025	0.82	+0.9
2,047,124	+		BBR_RS19025	0.83	-1.3
2,047,124	+		BBR_RS19025	0.83	+1.1
2,047,184	+		BBR_RS19025	0.88	-0.3
2,047,184	+		BBR_RS19025	0.88	-0.5
2,047,191	+		BBR_RS19025	0.88	+0.6
2,047,192	-		BBR_RS19025	0.88	+0.6
2,047,192	-		BBR_RS19025	0.88	+0.2
2,047,231	+				+0.7
2,047,231	+				-1.3
2,047,236	+				+0.2
2,047,236	+				-0.0
2,047,246	+				+0.1
2,047,246	+				+1.6
2,047,249	-				+0.1
2,047,256	+				+0.2
2,047,256	+				+1.4
2,047,264	-				+0.0
2,047,266	-				-0.1
2,047,281	-				+0.9
2,047,293	-				-0.6
2,047,293	-				+0.3
2,047,311	-				-0.4
2,047,322	+				+0.3
2,047,322	+				-0.1
2,047,332	+				-0.1
2,047,355	+				+0.1
2,047,355	+				-0.5
2,047,355	+				+0.4
2,047,355	+				-1.4
2,047,363	-				-1.0
2,047,363	-				-0.6
2,047,363	-				-0.9
2,047,363	-				-0.3
2,047,363	-				-0.0
2,047,486	-				-0.5
2,047,492	+				+0.8
2,047,533	+				-1.5
2,047,537	+				-2.1
2,047,537	+				-3.4
2,047,537	+				-1.7
2,047,538	-				-1.9
2,047,555	+				-2.4
2,047,582	+				+0.3
2,047,582	+				-0.1
2,047,582	+				-1.4
2,047,582	+				-3.0
2,047,582	+				-0.7
2,047,588	+				-0.8
2,047,590	-				-2.1
2,047,595	+				-2.0
2,047,595	+				-1.1
2,047,601	-				-2.3
2,047,612	+				-1.7
2,047,651	+		BBR_RS19030	0.11	-0.8
2,047,651	-		BBR_RS19030	0.11	-0.3
2,047,659	-		BBR_RS19030	0.12	-1.5
2,047,671	-		BBR_RS19030	0.13	-0.7
2,047,678	-		BBR_RS19030	0.13	-0.2
2,047,678	-		BBR_RS19030	0.13	-0.7
2,047,678	-		BBR_RS19030	0.13	-0.8
2,047,678	-		BBR_RS19030	0.13	-1.0
2,047,678	-		BBR_RS19030	0.13	-0.5
2,047,685	+		BBR_RS19030	0.14	-1.3
2,047,685	-		BBR_RS19030	0.14	-0.9
2,047,690	-		BBR_RS19030	0.14	-2.2
2,047,691	-		BBR_RS19030	0.15	-2.9
2,047,704	+		BBR_RS19030	0.16	-1.6
2,047,708	+		BBR_RS19030	0.16	-2.7
2,047,716	-		BBR_RS19030	0.17	-1.6
2,047,725	+		BBR_RS19030	0.18	-2.4
2,047,761	+		BBR_RS19030	0.21	-1.0
2,047,769	-		BBR_RS19030	0.21	-1.1
2,047,802	-		BBR_RS19030	0.24	-0.8
2,047,823	+		BBR_RS19030	0.26	-2.2
2,047,831	+		BBR_RS19030	0.27	-2.0
2,047,834	-		BBR_RS19030	0.27	-0.6
2,047,841	+		BBR_RS19030	0.28	-2.0
2,047,849	-		BBR_RS19030	0.29	-0.6
2,047,849	-		BBR_RS19030	0.29	-1.0
2,047,851	+		BBR_RS19030	0.29	-2.5
2,047,851	+		BBR_RS19030	0.29	-1.2
2,047,851	+		BBR_RS19030	0.29	-2.2
2,047,851	+		BBR_RS19030	0.29	-1.8
2,047,859	-		BBR_RS19030	0.29	-2.3
2,047,859	-		BBR_RS19030	0.29	-0.8
2,047,859	-		BBR_RS19030	0.29	-0.9
2,047,859	-		BBR_RS19030	0.29	-1.6
2,047,861	+		BBR_RS19030	0.30	-2.9
2,047,861	-		BBR_RS19030	0.30	-2.1
2,047,869	-		BBR_RS19030	0.30	-1.3
2,047,875	-		BBR_RS19030	0.31	+0.5
2,047,890	+		BBR_RS19030	0.32	+0.2
2,047,890	+		BBR_RS19030	0.32	-0.9
2,047,898	+		BBR_RS19030	0.33	-1.3
2,047,899	+		BBR_RS19030	0.33	-1.2
2,047,899	-		BBR_RS19030	0.33	-1.2
2,047,906	-		BBR_RS19030	0.34	-0.5
2,047,961	+		BBR_RS19030	0.38	-1.9
2,047,961	+		BBR_RS19030	0.38	+0.0
2,047,961	+		BBR_RS19030	0.38	-1.7
2,047,969	+		BBR_RS19030	0.39	-1.0
2,047,969	-		BBR_RS19030	0.39	-1.0
2,047,969	-		BBR_RS19030	0.39	-1.4
2,047,969	-		BBR_RS19030	0.39	-1.7
2,047,980	+		BBR_RS19030	0.40	-1.6
2,047,980	+		BBR_RS19030	0.40	-1.2
2,047,988	+		BBR_RS19030	0.41	-1.8
2,047,994	-		BBR_RS19030	0.41	-1.2
2,048,002	+		BBR_RS19030	0.42	-2.3
2,048,003	+		BBR_RS19030	0.42	-2.1
2,048,014	-		BBR_RS19030	0.43	-1.7
2,048,025	-		BBR_RS19030	0.44	-1.7
2,048,050	+		BBR_RS19030	0.46	-2.5
2,048,055	+		BBR_RS19030	0.47	+0.4
2,048,056	-		BBR_RS19030	0.47	-1.7
2,048,063	-		BBR_RS19030	0.48	-1.0
2,048,063	-		BBR_RS19030	0.48	-1.6
2,048,063	-		BBR_RS19030	0.48	-2.5
2,048,078	-		BBR_RS19030	0.49	-0.2
2,048,080	-		BBR_RS19030	0.49	-2.0
2,048,083	+		BBR_RS19030	0.49	-1.5
2,048,105	-		BBR_RS19030	0.51	-1.8
2,048,110	+		BBR_RS19030	0.52	-1.8
2,048,111	-		BBR_RS19030	0.52	-0.8
2,048,131	+		BBR_RS19030	0.54	-0.9
2,048,132	+		BBR_RS19030	0.54	-2.3
2,048,135	-		BBR_RS19030	0.54	-1.7
2,048,139	-		BBR_RS19030	0.54	-1.9
2,048,140	-		BBR_RS19030	0.54	-0.9
2,048,140	-		BBR_RS19030	0.54	-1.2
2,048,162	+		BBR_RS19030	0.56	-2.5
2,048,170	-		BBR_RS19030	0.57	-1.0
2,048,170	-		BBR_RS19030	0.57	-3.1
2,048,170	-		BBR_RS19030	0.57	-2.3
2,048,170	-		BBR_RS19030	0.57	-1.6
2,048,192	-		BBR_RS19030	0.59	-2.4
2,048,194	-		BBR_RS19030	0.59	-2.7
2,048,194	-		BBR_RS19030	0.59	-0.3
2,048,230	-		BBR_RS19030	0.62	-0.6
2,048,230	-		BBR_RS19030	0.62	-2.5
2,048,235	-		BBR_RS19030	0.63	-2.3
2,048,237	+		BBR_RS19030	0.63	-0.3
2,048,242	+		BBR_RS19030	0.63	-2.6
2,048,272	+		BBR_RS19030	0.66	-3.3
2,048,290	-		BBR_RS19030	0.68	-2.5
2,048,298	+		BBR_RS19030	0.68	-2.4
2,048,302	+		BBR_RS19030	0.69	-0.4
2,048,302	+		BBR_RS19030	0.69	-1.0
2,048,302	-		BBR_RS19030	0.69	-1.7
2,048,307	+		BBR_RS19030	0.69	-0.3
2,048,315	+		BBR_RS19030	0.70	-2.7
2,048,331	+		BBR_RS19030	0.71	-1.0
2,048,331	+		BBR_RS19030	0.71	-2.4
2,048,331	+		BBR_RS19030	0.71	-1.2
2,048,336	+		BBR_RS19030	0.72	+0.4
2,048,339	-		BBR_RS19030	0.72	-0.1
2,048,339	-		BBR_RS19030	0.72	+0.6
2,048,339	-		BBR_RS19030	0.72	-1.9
2,048,384	-		BBR_RS19030	0.76	-0.7
2,048,384	-		BBR_RS19030	0.76	-1.2
2,048,390	+		BBR_RS19030	0.77	-2.5
2,048,435	+		BBR_RS19030	0.80	-1.9
2,048,444	+		BBR_RS19030	0.81	-0.3
2,048,444	+		BBR_RS19030	0.81	-1.1
2,048,451	+		BBR_RS19030	0.82	-2.0
2,048,452	-		BBR_RS19030	0.82	-1.7
2,048,452	-		BBR_RS19030	0.82	-1.0
2,048,452	-		BBR_RS19030	0.82	-0.7
2,048,453	+		BBR_RS19030	0.82	-1.5
2,048,461	+		BBR_RS19030	0.83	-2.5
2,048,461	-		BBR_RS19030	0.83	-1.3
2,048,462	-		BBR_RS19030	0.83	-1.1
2,048,462	-		BBR_RS19030	0.83	-1.9
2,048,462	-		BBR_RS19030	0.83	-0.7
2,048,463	+		BBR_RS19030	0.83	-2.2
2,048,503	+		BBR_RS19030	0.87	-2.4
2,048,519	-		BBR_RS19030	0.88	-1.7
2,048,541	-		BBR_RS19030	0.90	-1.8
2,048,544	-				-0.7
2,048,553	+				-2.0
2,048,553	+				-0.4
2,048,560	+				+0.0
2,048,560	+				-2.1
2,048,560	+				-0.3
2,048,561	+				-2.3
2,048,568	-				-1.7
2,048,568	-				-0.7
2,048,568	-				-2.5
2,048,568	-				-1.1
2,048,570	+				-2.3
2,048,602	+				-1.0
2,048,610	-				-1.4
2,048,612	+				-2.3
2,048,620	-				-2.7
2,048,631	+				-0.0
2,048,631	+				-2.0
2,048,631	+				-1.6
2,048,631	+				-1.0
2,048,639	+				-0.3
2,048,639	+				-0.2
2,048,639	+				-1.1
2,048,639	+				-3.1
2,048,639	-				-0.2
2,048,639	-				-2.1
2,048,639	-				-2.0
2,048,639	-				-0.1
2,048,639	-				-0.4
2,048,639	-				-1.1
2,048,639	-				-0.8
2,048,639	-				-1.6
2,048,647	-				-2.2
2,048,647	-				-2.9
2,048,647	-				-3.4
2,048,647	-				-2.0
2,048,647	-				-1.4
2,048,680	+				-0.5
2,048,685	+				-0.2
2,048,690	+				-0.9
2,048,690	+				-0.1
2,048,693	-				+0.5
2,048,698	-				+0.5
2,048,753	+				+0.2
2,048,753	+				-0.4
2,048,774	-				-1.0
2,048,774	-				+0.1
2,048,774	-				-0.5
2,048,776	-				+0.6
2,048,781	-				+0.1
2,048,781	-				-0.5
2,048,813	+				+0.2
2,048,830	-				+0.2
2,048,832	+				-0.1
2,048,832	+				-0.1
2,048,832	+				+0.2
2,048,832	+				-0.5
2,048,832	+				+0.2
2,048,832	+				+0.1
2,048,832	+				-0.8
2,048,838	-				+0.3
2,048,840	-				-0.6
2,048,840	-				+1.7
2,048,840	-				+0.5
2,048,855	-				-1.0
2,048,863	+				-0.2
2,048,863	+				-0.2
2,048,868	-				+0.2
2,048,880	-				+0.2
2,048,889	+				+0.6
2,048,889	+				+1.1
2,048,897	-				+0.2
2,048,911	-				-1.0
2,048,930	-				+0.6
2,048,931	-				-0.6
2,048,968	+				-0.4
2,048,971	-				+0.2
2,048,976	-				-0.1
2,048,983	-				+0.4
2,048,983	-				+0.2
2,048,990	+				-0.0
2,048,999	+				+0.3
2,048,999	+				-0.2
2,048,999	+				-0.3
2,049,007	-				-0.7
2,049,007	-				-0.5
2,049,008	-				+1.0
2,049,043	-				-0.6
2,049,089	+				+1.3
2,049,101	+				+0.1
2,049,104	-				-0.3
2,049,142	-				+0.7
2,049,154	-				-0.2
2,049,157	+				-1.4
2,049,159	-				-0.5
2,049,165	+				-0.6
2,049,165	+				-0.0
2,049,165	+				+0.3
2,049,175	+				-1.2
2,049,405	+				+0.2
2,049,414	+				+1.2
2,049,451	+				+0.3
2,049,459	-				-0.3
2,049,463	+				+0.4
2,049,463	+				-0.3
2,049,463	+				-0.4
2,049,465	-				+0.8
2,049,465	-				-0.7
2,049,471	-				-0.9
2,049,471	-				-0.3
2,049,474	+				+1.0
2,049,476	-				+0.2
2,049,492	+				-0.1
2,049,500	+				-0.4
2,049,500	-				+0.5
2,049,500	-				+0.6
2,049,505	+				-0.9
2,049,506	-				+0.1
2,049,511	-				-0.2
2,049,513	-				+0.9
2,049,526	+		BBR_RS19040	0.10	+0.1
2,049,541	-		BBR_RS19040	0.12	-0.9
2,049,557	-		BBR_RS19040	0.14	+0.5
2,049,562	-		BBR_RS19040	0.15	-0.0
2,049,582	-		BBR_RS19040	0.18	+0.1
2,049,582	-		BBR_RS19040	0.18	+0.1
2,049,589	+		BBR_RS19040	0.19	-0.6
2,049,607	+		BBR_RS19040	0.22	+0.2
2,049,617	+		BBR_RS19040	0.23	+0.1
2,049,625	-		BBR_RS19040	0.24	+0.3
2,049,625	-		BBR_RS19040	0.24	-0.8
2,049,641	-		BBR_RS19040	0.26	-0.8