Experiment: reconstituted MRS with Sialyllacto-N-tetraose a 5.0 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16295 and BBR_RS16300 are separated by 83 nucleotides BBR_RS16300 and BBR_RS16305 are separated by 234 nucleotides BBR_RS16305 and BBR_RS16310 are separated by 74 nucleotides BBR_RS16310 and BBR_RS16315 are separated by 171 nucleotides
BBR_RS16295: BBR_RS16295 - DNA methyltransferase, at 1,403,192 to 1,404,463
_RS16295
BBR_RS16300: BBR_RS16300 - pyridoxamine 5'-phosphate oxidase family protein, at 1,404,547 to 1,404,951
_RS16300
BBR_RS16305: BBR_RS16305 - EamA family transporter, at 1,405,186 to 1,406,121
_RS16305
BBR_RS16310: BBR_RS16310 - hypothetical protein, at 1,406,196 to 1,406,894
_RS16310
BBR_RS16315: BBR_RS16315 - hypothetical protein, at 1,407,066 to 1,408,268
_RS16315
Position (kb)
1405
1406
1407 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1404.225 kb on - strand, within BBR_RS16295 at 1404.340 kb on - strand at 1404.340 kb on - strand at 1404.414 kb on - strand at 1404.428 kb on + strand at 1404.517 kb on + strand at 1404.520 kb on - strand at 1404.523 kb on - strand at 1404.525 kb on - strand at 1404.525 kb on - strand at 1404.525 kb on - strand at 1404.525 kb on - strand at 1404.548 kb on - strand at 1404.548 kb on - strand at 1404.667 kb on - strand, within BBR_RS16300 at 1404.667 kb on - strand, within BBR_RS16300 at 1404.667 kb on - strand, within BBR_RS16300 at 1404.667 kb on - strand, within BBR_RS16300 at 1404.667 kb on - strand, within BBR_RS16300 at 1404.667 kb on - strand, within BBR_RS16300 at 1404.667 kb on - strand, within BBR_RS16300 at 1404.667 kb on - strand, within BBR_RS16300 at 1404.702 kb on - strand, within BBR_RS16300 at 1404.762 kb on + strand, within BBR_RS16300 at 1404.765 kb on - strand, within BBR_RS16300 at 1404.781 kb on - strand, within BBR_RS16300 at 1404.782 kb on + strand, within BBR_RS16300 at 1404.790 kb on + strand, within BBR_RS16300 at 1404.798 kb on - strand, within BBR_RS16300 at 1404.860 kb on - strand, within BBR_RS16300 at 1404.887 kb on - strand, within BBR_RS16300 at 1404.952 kb on + strand at 1404.993 kb on + strand at 1405.052 kb on + strand at 1405.096 kb on - strand at 1405.187 kb on - strand at 1405.188 kb on + strand at 1405.188 kb on + strand at 1405.196 kb on - strand at 1405.255 kb on + strand at 1405.274 kb on + strand at 1405.277 kb on - strand at 1405.279 kb on + strand at 1405.281 kb on + strand, within BBR_RS16305 at 1405.287 kb on - strand, within BBR_RS16305 at 1405.287 kb on - strand, within BBR_RS16305 at 1405.291 kb on + strand, within BBR_RS16305 at 1405.341 kb on + strand, within BBR_RS16305 at 1405.348 kb on - strand, within BBR_RS16305 at 1405.393 kb on - strand, within BBR_RS16305 at 1405.492 kb on - strand, within BBR_RS16305 at 1405.499 kb on + strand, within BBR_RS16305 at 1405.499 kb on + strand, within BBR_RS16305 at 1405.499 kb on + strand, within BBR_RS16305 at 1405.499 kb on + strand, within BBR_RS16305 at 1405.499 kb on + strand, within BBR_RS16305 at 1405.504 kb on + strand, within BBR_RS16305 at 1405.507 kb on - strand, within BBR_RS16305 at 1405.728 kb on - strand, within BBR_RS16305 at 1405.730 kb on + strand, within BBR_RS16305 at 1405.745 kb on - strand, within BBR_RS16305 at 1405.798 kb on + strand, within BBR_RS16305 at 1405.816 kb on + strand, within BBR_RS16305 at 1405.836 kb on + strand, within BBR_RS16305 at 1405.858 kb on + strand, within BBR_RS16305 at 1405.895 kb on - strand, within BBR_RS16305 at 1405.959 kb on - strand, within BBR_RS16305 at 1405.963 kb on + strand, within BBR_RS16305 at 1405.980 kb on + strand, within BBR_RS16305 at 1406.008 kb on - strand, within BBR_RS16305 at 1406.010 kb on + strand, within BBR_RS16305 at 1406.010 kb on + strand, within BBR_RS16305 at 1406.010 kb on + strand, within BBR_RS16305 at 1406.010 kb on + strand, within BBR_RS16305 at 1406.010 kb on + strand, within BBR_RS16305 at 1406.010 kb on + strand, within BBR_RS16305 at 1406.010 kb on + strand, within BBR_RS16305 at 1406.012 kb on + strand, within BBR_RS16305 at 1406.018 kb on - strand, within BBR_RS16305 at 1406.018 kb on - strand, within BBR_RS16305 at 1406.018 kb on - strand, within BBR_RS16305 at 1406.018 kb on - strand, within BBR_RS16305 at 1406.018 kb on - strand, within BBR_RS16305 at 1406.018 kb on - strand, within BBR_RS16305 at 1406.018 kb on - strand, within BBR_RS16305 at 1406.018 kb on - strand, within BBR_RS16305 at 1406.018 kb on - strand, within BBR_RS16305 at 1406.018 kb on - strand, within BBR_RS16305 at 1406.018 kb on - strand, within BBR_RS16305 at 1406.018 kb on - strand, within BBR_RS16305 at 1406.079 kb on - strand at 1406.138 kb on + strand at 1406.158 kb on - strand at 1406.194 kb on + strand at 1406.260 kb on + strand at 1406.268 kb on - strand, within BBR_RS16310 at 1406.270 kb on + strand, within BBR_RS16310 at 1406.270 kb on + strand, within BBR_RS16310 at 1406.273 kb on - strand, within BBR_RS16310 at 1406.273 kb on - strand, within BBR_RS16310 at 1406.278 kb on - strand, within BBR_RS16310 at 1406.314 kb on - strand, within BBR_RS16310 at 1406.316 kb on + strand, within BBR_RS16310 at 1406.341 kb on - strand, within BBR_RS16310 at 1406.365 kb on + strand, within BBR_RS16310 at 1406.384 kb on + strand, within BBR_RS16310 at 1406.392 kb on - strand, within BBR_RS16310 at 1406.515 kb on + strand, within BBR_RS16310 at 1406.523 kb on - strand, within BBR_RS16310 at 1406.529 kb on + strand, within BBR_RS16310 at 1406.531 kb on + strand, within BBR_RS16310 at 1406.531 kb on + strand, within BBR_RS16310 at 1406.539 kb on - strand, within BBR_RS16310 at 1406.580 kb on + strand, within BBR_RS16310 at 1406.662 kb on - strand, within BBR_RS16310 at 1406.662 kb on - strand, within BBR_RS16310 at 1406.710 kb on + strand, within BBR_RS16310 at 1406.710 kb on + strand, within BBR_RS16310 at 1406.764 kb on - strand, within BBR_RS16310 at 1406.834 kb on + strand at 1407.035 kb on + strand at 1407.035 kb on + strand at 1407.115 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Sialyllacto-N-tetraose a 5.0 mM remove 1,404,225 - BBR_RS16295 0.81 -0.7 1,404,340 - -0.9 1,404,340 - -0.3 1,404,414 - -0.7 1,404,428 + -0.3 1,404,517 + -0.7 1,404,520 - -0.1 1,404,523 - -0.4 1,404,525 - -0.6 1,404,525 - +0.8 1,404,525 - -0.1 1,404,525 - -0.5 1,404,548 - +0.6 1,404,548 - -1.5 1,404,667 - BBR_RS16300 0.30 +0.3 1,404,667 - BBR_RS16300 0.30 -0.5 1,404,667 - BBR_RS16300 0.30 +0.1 1,404,667 - BBR_RS16300 0.30 -0.0 1,404,667 - BBR_RS16300 0.30 -0.1 1,404,667 - BBR_RS16300 0.30 -0.9 1,404,667 - BBR_RS16300 0.30 -0.4 1,404,667 - BBR_RS16300 0.30 +0.2 1,404,702 - BBR_RS16300 0.38 -0.4 1,404,762 + BBR_RS16300 0.53 +0.0 1,404,765 - BBR_RS16300 0.54 -0.7 1,404,781 - BBR_RS16300 0.58 +0.6 1,404,782 + BBR_RS16300 0.58 +1.6 1,404,790 + BBR_RS16300 0.60 +0.2 1,404,798 - BBR_RS16300 0.62 +0.6 1,404,860 - BBR_RS16300 0.77 +0.9 1,404,887 - BBR_RS16300 0.84 +1.1 1,404,952 + +1.8 1,404,993 + +0.0 1,405,052 + +0.1 1,405,096 - +1.0 1,405,187 - -0.9 1,405,188 + +0.3 1,405,188 + -1.5 1,405,196 - -0.1 1,405,255 + -0.4 1,405,274 + +0.4 1,405,277 - +0.9 1,405,279 + +0.6 1,405,281 + BBR_RS16305 0.10 +0.2 1,405,287 - BBR_RS16305 0.11 +1.2 1,405,287 - BBR_RS16305 0.11 +0.1 1,405,291 + BBR_RS16305 0.11 -0.8 1,405,341 + BBR_RS16305 0.17 +0.8 1,405,348 - BBR_RS16305 0.17 +0.6 1,405,393 - BBR_RS16305 0.22 +1.0 1,405,492 - BBR_RS16305 0.33 +0.3 1,405,499 + BBR_RS16305 0.33 -0.2 1,405,499 + BBR_RS16305 0.33 +0.7 1,405,499 + BBR_RS16305 0.33 -0.3 1,405,499 + BBR_RS16305 0.33 +0.3 1,405,499 + BBR_RS16305 0.33 -0.1 1,405,504 + BBR_RS16305 0.34 -0.2 1,405,507 - BBR_RS16305 0.34 -1.1 1,405,728 - BBR_RS16305 0.58 -1.1 1,405,730 + BBR_RS16305 0.58 +0.3 1,405,745 - BBR_RS16305 0.60 +1.0 1,405,798 + BBR_RS16305 0.65 -1.8 1,405,816 + BBR_RS16305 0.67 -2.0 1,405,836 + BBR_RS16305 0.69 +0.2 1,405,858 + BBR_RS16305 0.72 -0.3 1,405,895 - BBR_RS16305 0.76 +0.1 1,405,959 - BBR_RS16305 0.83 +2.0 1,405,963 + BBR_RS16305 0.83 -0.0 1,405,980 + BBR_RS16305 0.85 +0.8 1,406,008 - BBR_RS16305 0.88 +0.1 1,406,010 + BBR_RS16305 0.88 +2.6 1,406,010 + BBR_RS16305 0.88 +0.3 1,406,010 + BBR_RS16305 0.88 -0.0 1,406,010 + BBR_RS16305 0.88 +1.0 1,406,010 + BBR_RS16305 0.88 -0.0 1,406,010 + BBR_RS16305 0.88 -0.2 1,406,010 + BBR_RS16305 0.88 -0.2 1,406,012 + BBR_RS16305 0.88 +2.0 1,406,018 - BBR_RS16305 0.89 +0.4 1,406,018 - BBR_RS16305 0.89 +0.2 1,406,018 - BBR_RS16305 0.89 -1.3 1,406,018 - BBR_RS16305 0.89 -0.6 1,406,018 - BBR_RS16305 0.89 +0.7 1,406,018 - BBR_RS16305 0.89 -0.1 1,406,018 - BBR_RS16305 0.89 +0.3 1,406,018 - BBR_RS16305 0.89 +0.4 1,406,018 - BBR_RS16305 0.89 -0.3 1,406,018 - BBR_RS16305 0.89 -0.4 1,406,018 - BBR_RS16305 0.89 -1.2 1,406,018 - BBR_RS16305 0.89 -0.2 1,406,079 - +0.9 1,406,138 + -0.6 1,406,158 - +1.1 1,406,194 + -0.1 1,406,260 + +1.0 1,406,268 - BBR_RS16310 0.10 +2.2 1,406,270 + BBR_RS16310 0.11 +0.4 1,406,270 + BBR_RS16310 0.11 -0.8 1,406,273 - BBR_RS16310 0.11 +1.0 1,406,273 - BBR_RS16310 0.11 -1.1 1,406,278 - BBR_RS16310 0.12 +0.4 1,406,314 - BBR_RS16310 0.17 -0.0 1,406,316 + BBR_RS16310 0.17 -0.3 1,406,341 - BBR_RS16310 0.21 -0.0 1,406,365 + BBR_RS16310 0.24 +0.2 1,406,384 + BBR_RS16310 0.27 -0.0 1,406,392 - BBR_RS16310 0.28 +0.5 1,406,515 + BBR_RS16310 0.46 +0.2 1,406,523 - BBR_RS16310 0.47 +0.0 1,406,529 + BBR_RS16310 0.48 +0.4 1,406,531 + BBR_RS16310 0.48 -0.0 1,406,531 + BBR_RS16310 0.48 +0.4 1,406,539 - BBR_RS16310 0.49 -0.2 1,406,580 + BBR_RS16310 0.55 +0.4 1,406,662 - BBR_RS16310 0.67 -0.5 1,406,662 - BBR_RS16310 0.67 -0.0 1,406,710 + BBR_RS16310 0.74 -0.8 1,406,710 + BBR_RS16310 0.74 -0.2 1,406,764 - BBR_RS16310 0.81 -0.0 1,406,834 + -0.4 1,407,035 + -0.5 1,407,035 + +0.4 1,407,115 - +0.1
Or see this region's nucleotide sequence