Experiment: reconstituted MRS with Sialyllacto-N-tetraose a 5.0 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13905 and BBR_RS13910 overlap by 4 nucleotides BBR_RS13910 and BBR_RS13915 are separated by 155 nucleotides BBR_RS13915 and BBR_RS20805 are separated by 366 nucleotides
BBR_RS13905: BBR_RS13905 - ornithine carbamoyltransferase, at 899,670 to 900,635
_RS13905
BBR_RS13910: BBR_RS13910 - arginine repressor, at 900,632 to 901,144
_RS13910
BBR_RS13915: BBR_RS13915 - argininosuccinate synthase, at 901,300 to 902,538
_RS13915
BBR_RS20805: BBR_RS20805 - hypothetical protein, at 902,905 to 903,759
_RS20805
Position (kb)
901
902
903 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 900.310 kb on + strand, within BBR_RS13905 at 900.310 kb on + strand, within BBR_RS13905 at 900.410 kb on + strand, within BBR_RS13905 at 900.418 kb on - strand, within BBR_RS13905 at 900.425 kb on + strand, within BBR_RS13905 at 900.425 kb on + strand, within BBR_RS13905 at 900.539 kb on + strand at 900.540 kb on + strand at 900.826 kb on + strand, within BBR_RS13910 at 900.932 kb on + strand, within BBR_RS13910 at 900.985 kb on + strand, within BBR_RS13910 at 901.159 kb on + strand at 901.167 kb on - strand at 901.171 kb on + strand at 901.179 kb on - strand at 901.190 kb on - strand at 901.205 kb on + strand at 901.213 kb on - strand at 901.316 kb on + strand at 901.326 kb on + strand at 901.329 kb on - strand at 901.339 kb on + strand at 901.339 kb on - strand at 901.417 kb on + strand at 901.417 kb on + strand at 901.417 kb on + strand at 901.417 kb on + strand at 901.417 kb on + strand at 901.441 kb on - strand, within BBR_RS13915 at 901.442 kb on + strand, within BBR_RS13915 at 901.442 kb on + strand, within BBR_RS13915 at 901.442 kb on - strand, within BBR_RS13915 at 901.489 kb on + strand, within BBR_RS13915 at 901.539 kb on + strand, within BBR_RS13915 at 901.539 kb on + strand, within BBR_RS13915 at 901.567 kb on + strand, within BBR_RS13915 at 901.573 kb on - strand, within BBR_RS13915 at 901.606 kb on + strand, within BBR_RS13915 at 901.629 kb on - strand, within BBR_RS13915 at 901.676 kb on - strand, within BBR_RS13915 at 901.693 kb on + strand, within BBR_RS13915 at 901.693 kb on - strand, within BBR_RS13915 at 901.719 kb on - strand, within BBR_RS13915 at 901.728 kb on - strand, within BBR_RS13915 at 901.750 kb on + strand, within BBR_RS13915 at 901.774 kb on + strand, within BBR_RS13915 at 901.799 kb on + strand, within BBR_RS13915 at 901.809 kb on + strand, within BBR_RS13915 at 901.839 kb on + strand, within BBR_RS13915 at 901.851 kb on - strand, within BBR_RS13915 at 901.905 kb on + strand, within BBR_RS13915 at 901.905 kb on + strand, within BBR_RS13915 at 901.911 kb on + strand, within BBR_RS13915 at 901.919 kb on - strand, within BBR_RS13915 at 901.940 kb on - strand, within BBR_RS13915 at 901.998 kb on - strand, within BBR_RS13915 at 902.019 kb on - strand, within BBR_RS13915 at 902.021 kb on + strand, within BBR_RS13915 at 902.094 kb on - strand, within BBR_RS13915 at 902.105 kb on + strand, within BBR_RS13915 at 902.115 kb on + strand, within BBR_RS13915 at 902.122 kb on + strand, within BBR_RS13915 at 902.123 kb on - strand, within BBR_RS13915 at 902.123 kb on - strand, within BBR_RS13915 at 902.123 kb on - strand, within BBR_RS13915 at 902.123 kb on - strand, within BBR_RS13915 at 902.132 kb on + strand, within BBR_RS13915 at 902.137 kb on + strand, within BBR_RS13915 at 902.142 kb on + strand, within BBR_RS13915 at 902.142 kb on + strand, within BBR_RS13915 at 902.161 kb on + strand, within BBR_RS13915 at 902.193 kb on - strand, within BBR_RS13915 at 902.217 kb on + strand, within BBR_RS13915 at 902.217 kb on + strand, within BBR_RS13915 at 902.217 kb on + strand, within BBR_RS13915 at 902.232 kb on + strand, within BBR_RS13915 at 902.232 kb on + strand, within BBR_RS13915 at 902.232 kb on + strand, within BBR_RS13915 at 902.326 kb on + strand, within BBR_RS13915 at 902.326 kb on + strand, within BBR_RS13915 at 902.326 kb on + strand, within BBR_RS13915 at 902.326 kb on + strand, within BBR_RS13915 at 902.326 kb on + strand, within BBR_RS13915 at 902.334 kb on - strand, within BBR_RS13915 at 902.347 kb on + strand, within BBR_RS13915 at 902.362 kb on + strand, within BBR_RS13915 at 902.362 kb on + strand, within BBR_RS13915 at 902.363 kb on + strand, within BBR_RS13915 at 902.422 kb on + strand at 902.451 kb on + strand at 902.507 kb on + strand at 902.554 kb on - strand at 902.567 kb on + strand at 902.575 kb on - strand at 902.599 kb on - strand at 902.601 kb on + strand at 902.604 kb on - strand at 902.604 kb on - strand at 902.609 kb on - strand at 902.609 kb on - strand at 902.618 kb on + strand at 902.755 kb on + strand at 902.821 kb on + strand at 902.834 kb on - strand at 902.893 kb on + strand at 902.906 kb on - strand at 902.933 kb on - strand at 902.949 kb on + strand at 903.006 kb on + strand, within BBR_RS20805 at 903.006 kb on + strand, within BBR_RS20805 at 903.014 kb on - strand, within BBR_RS20805 at 903.014 kb on - strand, within BBR_RS20805 at 903.039 kb on - strand, within BBR_RS20805 at 903.055 kb on + strand, within BBR_RS20805 at 903.084 kb on + strand, within BBR_RS20805 at 903.162 kb on - strand, within BBR_RS20805 at 903.177 kb on + strand, within BBR_RS20805 at 903.313 kb on - strand, within BBR_RS20805 at 903.326 kb on - strand, within BBR_RS20805 at 903.392 kb on + strand, within BBR_RS20805 at 903.400 kb on - strand, within BBR_RS20805
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Sialyllacto-N-tetraose a 5.0 mM remove 900,310 + BBR_RS13905 0.66 -2.6 900,310 + BBR_RS13905 0.66 +0.7 900,410 + BBR_RS13905 0.77 +0.0 900,418 - BBR_RS13905 0.77 +1.6 900,425 + BBR_RS13905 0.78 +0.5 900,425 + BBR_RS13905 0.78 -0.6 900,539 + -0.3 900,540 + -0.3 900,826 + BBR_RS13910 0.38 +1.9 900,932 + BBR_RS13910 0.58 +0.3 900,985 + BBR_RS13910 0.69 +0.2 901,159 + +0.5 901,167 - -0.5 901,171 + +1.3 901,179 - +0.9 901,190 - -0.9 901,205 + -0.6 901,213 - +0.3 901,316 + -0.0 901,326 + +0.6 901,329 - -0.8 901,339 + -0.0 901,339 - -0.5 901,417 + -0.6 901,417 + +0.3 901,417 + +0.0 901,417 + -0.3 901,417 + -0.7 901,441 - BBR_RS13915 0.11 -1.1 901,442 + BBR_RS13915 0.11 +0.3 901,442 + BBR_RS13915 0.11 +0.1 901,442 - BBR_RS13915 0.11 +0.6 901,489 + BBR_RS13915 0.15 -1.1 901,539 + BBR_RS13915 0.19 -0.6 901,539 + BBR_RS13915 0.19 -0.1 901,567 + BBR_RS13915 0.22 +0.6 901,573 - BBR_RS13915 0.22 +0.1 901,606 + BBR_RS13915 0.25 -0.4 901,629 - BBR_RS13915 0.27 +0.3 901,676 - BBR_RS13915 0.30 -0.2 901,693 + BBR_RS13915 0.32 +0.3 901,693 - BBR_RS13915 0.32 -0.7 901,719 - BBR_RS13915 0.34 -1.2 901,728 - BBR_RS13915 0.35 +0.8 901,750 + BBR_RS13915 0.36 +0.1 901,774 + BBR_RS13915 0.38 +0.5 901,799 + BBR_RS13915 0.40 -0.3 901,809 + BBR_RS13915 0.41 -0.4 901,839 + BBR_RS13915 0.44 +0.6 901,851 - BBR_RS13915 0.44 -0.2 901,905 + BBR_RS13915 0.49 -0.6 901,905 + BBR_RS13915 0.49 -0.2 901,911 + BBR_RS13915 0.49 -0.0 901,919 - BBR_RS13915 0.50 +0.2 901,940 - BBR_RS13915 0.52 +0.6 901,998 - BBR_RS13915 0.56 +0.5 902,019 - BBR_RS13915 0.58 +0.2 902,021 + BBR_RS13915 0.58 -0.6 902,094 - BBR_RS13915 0.64 +1.3 902,105 + BBR_RS13915 0.65 -0.9 902,115 + BBR_RS13915 0.66 -0.0 902,122 + BBR_RS13915 0.66 -1.3 902,123 - BBR_RS13915 0.66 +0.5 902,123 - BBR_RS13915 0.66 -0.1 902,123 - BBR_RS13915 0.66 -0.8 902,123 - BBR_RS13915 0.66 +0.4 902,132 + BBR_RS13915 0.67 -0.4 902,137 + BBR_RS13915 0.68 -0.2 902,142 + BBR_RS13915 0.68 +0.7 902,142 + BBR_RS13915 0.68 +0.4 902,161 + BBR_RS13915 0.69 +0.3 902,193 - BBR_RS13915 0.72 -0.3 902,217 + BBR_RS13915 0.74 -0.1 902,217 + BBR_RS13915 0.74 -0.3 902,217 + BBR_RS13915 0.74 +0.1 902,232 + BBR_RS13915 0.75 -0.2 902,232 + BBR_RS13915 0.75 +0.6 902,232 + BBR_RS13915 0.75 -0.1 902,326 + BBR_RS13915 0.83 -0.1 902,326 + BBR_RS13915 0.83 -0.5 902,326 + BBR_RS13915 0.83 +0.0 902,326 + BBR_RS13915 0.83 +0.3 902,326 + BBR_RS13915 0.83 -0.1 902,334 - BBR_RS13915 0.83 +0.1 902,347 + BBR_RS13915 0.85 +0.3 902,362 + BBR_RS13915 0.86 +0.2 902,362 + BBR_RS13915 0.86 +0.3 902,363 + BBR_RS13915 0.86 +0.5 902,422 + -0.3 902,451 + -0.2 902,507 + -1.3 902,554 - -2.1 902,567 + -0.7 902,575 - +0.2 902,599 - -0.8 902,601 + -0.1 902,604 - -1.9 902,604 - -0.5 902,609 - +0.2 902,609 - -0.4 902,618 + +0.3 902,755 + +0.3 902,821 + +0.3 902,834 - -0.9 902,893 + +1.1 902,906 - -0.0 902,933 - +0.5 902,949 + +0.1 903,006 + BBR_RS20805 0.12 -0.1 903,006 + BBR_RS20805 0.12 -0.7 903,014 - BBR_RS20805 0.13 -0.3 903,014 - BBR_RS20805 0.13 +0.9 903,039 - BBR_RS20805 0.16 +0.5 903,055 + BBR_RS20805 0.18 -0.1 903,084 + BBR_RS20805 0.21 +0.5 903,162 - BBR_RS20805 0.30 +0.1 903,177 + BBR_RS20805 0.32 +0.2 903,313 - BBR_RS20805 0.48 +0.5 903,326 - BBR_RS20805 0.49 -0.5 903,392 + BBR_RS20805 0.57 -0.7 903,400 - BBR_RS20805 0.58 +0.4
Or see this region's nucleotide sequence