Experiment: L-Methionine as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nifH and DvMF_2614 are separated by 101 nucleotides DvMF_2614 and DvMF_2615 overlap by 4 nucleotides DvMF_2615 and DvMF_2616 are separated by 231 nucleotides
DvMF_2613: nifH - nitrogenase reductase (RefSeq), at 3,302,404 to 3,303,234
nifH
DvMF_2614: DvMF_2614 - nitrogen regulatory protein P-II (RefSeq), at 3,303,336 to 3,303,686
_2614
DvMF_2615: DvMF_2615 - nitrogen regulatory protein P-II (RefSeq), at 3,303,683 to 3,304,057
_2615
DvMF_2616: DvMF_2616 - nitrogenase molybdenum-iron protein alpha chain (RefSeq), at 3,304,289 to 3,305,923
_2616
Position (kb)
3303
3304 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3302.380 kb on + strand at 3302.453 kb on - strand at 3302.455 kb on + strand at 3302.685 kb on - strand, within nifH at 3302.826 kb on + strand, within nifH at 3303.112 kb on + strand, within nifH at 3303.120 kb on - strand, within nifH at 3303.222 kb on + strand at 3303.222 kb on + strand at 3303.237 kb on - strand at 3303.269 kb on + strand at 3303.292 kb on + strand at 3303.637 kb on - strand, within DvMF_2614 at 3303.637 kb on - strand, within DvMF_2614 at 3303.681 kb on + strand at 3303.744 kb on - strand, within DvMF_2615 at 3303.790 kb on + strand, within DvMF_2615 at 3303.810 kb on - strand, within DvMF_2615 at 3303.837 kb on + strand, within DvMF_2615 at 3303.912 kb on - strand, within DvMF_2615 at 3303.951 kb on + strand, within DvMF_2615 at 3303.959 kb on - strand, within DvMF_2615 at 3304.102 kb on - strand at 3304.243 kb on + strand at 3304.308 kb on - strand at 3304.311 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine as nutrient remove 3,302,380 + +2.9 3,302,453 - +0.6 3,302,455 + +0.1 3,302,685 - nifH DvMF_2613 0.34 +0.8 3,302,826 + nifH DvMF_2613 0.51 +0.4 3,303,112 + nifH DvMF_2613 0.85 +0.2 3,303,120 - nifH DvMF_2613 0.86 +0.8 3,303,222 + +0.6 3,303,222 + +1.0 3,303,237 - -0.5 3,303,269 + +2.3 3,303,292 + +0.3 3,303,637 - DvMF_2614 0.86 +0.2 3,303,637 - DvMF_2614 0.86 -1.2 3,303,681 + +0.5 3,303,744 - DvMF_2615 0.16 +0.5 3,303,790 + DvMF_2615 0.29 -0.3 3,303,810 - DvMF_2615 0.34 +1.7 3,303,837 + DvMF_2615 0.41 -0.1 3,303,912 - DvMF_2615 0.61 +0.8 3,303,951 + DvMF_2615 0.71 +1.0 3,303,959 - DvMF_2615 0.74 +0.7 3,304,102 - +0.9 3,304,243 + +1.0 3,304,308 - +0.3 3,304,311 + +0.1
Or see this region's nucleotide sequence