Experiment: L-Methionine as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0462 and DvMF_0463 are separated by 62 nucleotides DvMF_0463 and DvMF_0464 are separated by 134 nucleotides DvMF_0464 and DvMF_0465 are separated by 120 nucleotides
DvMF_0462: DvMF_0462 - protein of unknown function DUF190 (RefSeq), at 541,348 to 541,743
_0462
DvMF_0463: DvMF_0463 - GCN5-related N-acetyltransferase (RefSeq), at 541,806 to 542,342
_0463
DvMF_0464: DvMF_0464 - nitrite and sulphite reductase 4Fe-4S region (RefSeq), at 542,477 to 543,154
_0464
DvMF_0465: DvMF_0465 - response regulator receiver modulated CheB methylesterase (RefSeq), at 543,275 to 544,345
_0465
Position (kb) 542
543
544 Strain fitness (log2 ratio) -1
0
1
2
3 at 541.641 kb on - strand, within DvMF_0462 at 541.738 kb on + strand at 542.136 kb on + strand, within DvMF_0463 at 542.555 kb on - strand, within DvMF_0464 at 542.786 kb on + strand, within DvMF_0464 at 542.809 kb on - strand, within DvMF_0464 at 542.896 kb on + strand, within DvMF_0464 at 542.904 kb on - strand, within DvMF_0464 at 542.966 kb on + strand, within DvMF_0464 at 543.220 kb on - strand at 543.266 kb on + strand at 543.274 kb on - strand at 543.362 kb on + strand at 543.383 kb on - strand, within DvMF_0465 at 543.399 kb on - strand, within DvMF_0465 at 543.399 kb on - strand, within DvMF_0465 at 543.781 kb on - strand, within DvMF_0465 at 543.862 kb on - strand, within DvMF_0465 at 544.149 kb on - strand, within DvMF_0465
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine as nutrient remove 541,641 - DvMF_0462 0.74 +1.5 541,738 + +0.3 542,136 + DvMF_0463 0.61 -0.5 542,555 - DvMF_0464 0.12 +0.7 542,786 + DvMF_0464 0.46 +0.3 542,809 - DvMF_0464 0.49 +0.5 542,896 + DvMF_0464 0.62 +1.4 542,904 - DvMF_0464 0.63 +0.5 542,966 + DvMF_0464 0.72 +1.3 543,220 - +0.4 543,266 + +0.3 543,274 - +0.0 543,362 + +1.1 543,383 - DvMF_0465 0.10 +2.3 543,399 - DvMF_0465 0.12 +1.2 543,399 - DvMF_0465 0.12 +2.4 543,781 - DvMF_0465 0.47 +2.0 543,862 - DvMF_0465 0.55 +1.4 544,149 - DvMF_0465 0.82 +3.3
Or see this region's nucleotide sequence