Experiment: m.b. Cephalothin 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2227 and Dshi_2228 are separated by 72 nucleotides Dshi_2228 and Dshi_2229 overlap by 4 nucleotides Dshi_2229 and Dshi_2230 are separated by 45 nucleotides
Dshi_2227: Dshi_2227 - FAD dependent oxidoreductase (RefSeq), at 2,350,024 to 2,351,328
_2227
Dshi_2228: Dshi_2228 - DegT/DnrJ/EryC1/StrS aminotransferase (RefSeq), at 2,351,401 to 2,352,594
_2228
Dshi_2229: Dshi_2229 - transcriptional regulator, LysR family (RefSeq), at 2,352,591 to 2,353,535
_2229
Dshi_2230: Dshi_2230 - polyhydroxyalkonate synthesis repressor, PhaR (RefSeq), at 2,353,581 to 2,354,141
_2230
Position (kb)
2351
2352
2353 Strain fitness (log2 ratio)
-2
-1
0
1 at 2350.414 kb on + strand, within Dshi_2227 at 2350.419 kb on + strand, within Dshi_2227 at 2350.461 kb on + strand, within Dshi_2227 at 2350.518 kb on - strand, within Dshi_2227 at 2350.598 kb on + strand, within Dshi_2227 at 2350.598 kb on - strand, within Dshi_2227 at 2350.653 kb on - strand, within Dshi_2227 at 2350.771 kb on + strand, within Dshi_2227 at 2350.841 kb on + strand, within Dshi_2227 at 2350.869 kb on - strand, within Dshi_2227 at 2351.297 kb on + strand at 2351.496 kb on - strand at 2351.501 kb on + strand at 2351.565 kb on + strand, within Dshi_2228 at 2351.573 kb on - strand, within Dshi_2228 at 2351.674 kb on + strand, within Dshi_2228 at 2351.689 kb on - strand, within Dshi_2228 at 2351.827 kb on - strand, within Dshi_2228 at 2351.859 kb on + strand, within Dshi_2228 at 2351.877 kb on + strand, within Dshi_2228 at 2351.902 kb on + strand, within Dshi_2228 at 2351.910 kb on - strand, within Dshi_2228 at 2352.116 kb on - strand, within Dshi_2228 at 2352.125 kb on + strand, within Dshi_2228 at 2352.243 kb on + strand, within Dshi_2228 at 2352.243 kb on + strand, within Dshi_2228 at 2352.248 kb on + strand, within Dshi_2228 at 2352.278 kb on - strand, within Dshi_2228 at 2352.384 kb on - strand, within Dshi_2228 at 2352.451 kb on - strand, within Dshi_2228 at 2352.500 kb on + strand at 2352.536 kb on + strand at 2352.625 kb on - strand at 2352.792 kb on - strand, within Dshi_2229 at 2352.824 kb on - strand, within Dshi_2229 at 2353.331 kb on + strand, within Dshi_2229 at 2353.349 kb on + strand, within Dshi_2229 at 2353.357 kb on - strand, within Dshi_2229 at 2353.375 kb on - strand, within Dshi_2229 at 2353.524 kb on + strand at 2353.524 kb on + strand at 2353.533 kb on - strand at 2353.566 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cephalothin 0.0001 mg/ml remove 2,350,414 + Dshi_2227 0.30 -1.1 2,350,419 + Dshi_2227 0.30 -1.9 2,350,461 + Dshi_2227 0.33 -0.1 2,350,518 - Dshi_2227 0.38 -0.6 2,350,598 + Dshi_2227 0.44 -1.6 2,350,598 - Dshi_2227 0.44 -1.4 2,350,653 - Dshi_2227 0.48 -1.7 2,350,771 + Dshi_2227 0.57 -1.7 2,350,841 + Dshi_2227 0.63 +0.5 2,350,869 - Dshi_2227 0.65 -0.1 2,351,297 + -0.5 2,351,496 - -0.1 2,351,501 + +0.7 2,351,565 + Dshi_2228 0.14 +0.0 2,351,573 - Dshi_2228 0.14 +0.2 2,351,674 + Dshi_2228 0.23 +0.1 2,351,689 - Dshi_2228 0.24 +0.1 2,351,827 - Dshi_2228 0.36 +0.9 2,351,859 + Dshi_2228 0.38 -0.1 2,351,877 + Dshi_2228 0.40 +0.3 2,351,902 + Dshi_2228 0.42 -0.4 2,351,910 - Dshi_2228 0.43 +0.5 2,352,116 - Dshi_2228 0.60 +0.5 2,352,125 + Dshi_2228 0.61 +0.2 2,352,243 + Dshi_2228 0.71 +0.1 2,352,243 + Dshi_2228 0.71 +1.2 2,352,248 + Dshi_2228 0.71 +1.2 2,352,278 - Dshi_2228 0.73 +0.3 2,352,384 - Dshi_2228 0.82 -0.1 2,352,451 - Dshi_2228 0.88 +0.2 2,352,500 + +0.3 2,352,536 + +0.4 2,352,625 - -0.2 2,352,792 - Dshi_2229 0.21 +0.5 2,352,824 - Dshi_2229 0.25 -0.7 2,353,331 + Dshi_2229 0.78 -1.5 2,353,349 + Dshi_2229 0.80 -0.5 2,353,357 - Dshi_2229 0.81 -0.6 2,353,375 - Dshi_2229 0.83 -0.7 2,353,524 + -0.1 2,353,524 + -0.3 2,353,533 - +0.2 2,353,566 + +0.0
Or see this region's nucleotide sequence