Experiment: selenate 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_671 and Pf6N2E2_672 are separated by 51 nucleotides Pf6N2E2_672 and Pf6N2E2_673 overlap by 8 nucleotides Pf6N2E2_673 and Pf6N2E2_674 are separated by 12 nucleotides
Pf6N2E2_671 - 2-methylaconitate racemase, at 776,215 to 777,243
_671
Pf6N2E2_672 - ABC-type polar amino acid transport system, ATPase component, at 777,295 to 778,017
_672
Pf6N2E2_673 - Probable permease of ABC transporter, at 778,010 to 778,660
_673
Pf6N2E2_674 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 778,673 to 779,338
_674
Position (kb) 777
778
779 Strain fitness (log2 ratio) -2
-1
0
1
2 at 776.328 kb on - strand, within Pf6N2E2_671 at 776.406 kb on + strand, within Pf6N2E2_671 at 776.406 kb on + strand, within Pf6N2E2_671 at 776.454 kb on + strand, within Pf6N2E2_671 at 776.454 kb on + strand, within Pf6N2E2_671 at 776.739 kb on - strand, within Pf6N2E2_671 at 777.217 kb on + strand at 777.217 kb on + strand at 777.218 kb on - strand at 777.218 kb on - strand at 777.494 kb on - strand, within Pf6N2E2_672 at 777.548 kb on + strand, within Pf6N2E2_672 at 777.548 kb on + strand, within Pf6N2E2_672 at 777.549 kb on - strand, within Pf6N2E2_672 at 778.078 kb on - strand, within Pf6N2E2_673 at 778.078 kb on - strand, within Pf6N2E2_673 at 778.081 kb on - strand, within Pf6N2E2_673 at 778.282 kb on - strand, within Pf6N2E2_673 at 778.282 kb on - strand, within Pf6N2E2_673 at 778.375 kb on - strand, within Pf6N2E2_673 at 778.623 kb on + strand at 778.624 kb on - strand at 778.651 kb on + strand at 778.651 kb on + strand at 778.652 kb on - strand at 778.740 kb on + strand, within Pf6N2E2_674 at 778.741 kb on - strand, within Pf6N2E2_674 at 778.741 kb on - strand, within Pf6N2E2_674 at 778.741 kb on - strand, within Pf6N2E2_674 at 778.741 kb on - strand, within Pf6N2E2_674 at 778.765 kb on - strand, within Pf6N2E2_674 at 778.765 kb on - strand, within Pf6N2E2_674 at 778.794 kb on + strand, within Pf6N2E2_674 at 778.795 kb on - strand, within Pf6N2E2_674 at 778.891 kb on - strand, within Pf6N2E2_674 at 778.891 kb on - strand, within Pf6N2E2_674
Per-strain Table
Position Strand Gene LocusTag Fraction selenate 0.5 M remove 776,328 - Pf6N2E2_671 0.11 +0.2 776,406 + Pf6N2E2_671 0.19 +0.1 776,406 + Pf6N2E2_671 0.19 +1.3 776,454 + Pf6N2E2_671 0.23 -0.2 776,454 + Pf6N2E2_671 0.23 +0.1 776,739 - Pf6N2E2_671 0.51 -0.3 777,217 + +0.3 777,217 + +0.5 777,218 - +0.5 777,218 - +0.6 777,494 - Pf6N2E2_672 0.28 -0.5 777,548 + Pf6N2E2_672 0.35 -0.5 777,548 + Pf6N2E2_672 0.35 -0.6 777,549 - Pf6N2E2_672 0.35 -0.1 778,078 - Pf6N2E2_673 0.10 -0.2 778,078 - Pf6N2E2_673 0.10 +0.5 778,081 - Pf6N2E2_673 0.11 +1.7 778,282 - Pf6N2E2_673 0.42 +0.6 778,282 - Pf6N2E2_673 0.42 +0.2 778,375 - Pf6N2E2_673 0.56 -0.8 778,623 + -1.8 778,624 - +1.8 778,651 + +0.2 778,651 + -0.6 778,652 - -1.1 778,740 + Pf6N2E2_674 0.10 -0.9 778,741 - Pf6N2E2_674 0.10 +0.6 778,741 - Pf6N2E2_674 0.10 +0.3 778,741 - Pf6N2E2_674 0.10 -1.8 778,741 - Pf6N2E2_674 0.10 +0.8 778,765 - Pf6N2E2_674 0.14 -0.4 778,765 - Pf6N2E2_674 0.14 +0.6 778,794 + Pf6N2E2_674 0.18 -0.6 778,795 - Pf6N2E2_674 0.18 +0.5 778,891 - Pf6N2E2_674 0.33 +0.6 778,891 - Pf6N2E2_674 0.33 -0.9
Or see this region's nucleotide sequence