Experiment: selenate 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_645 and Pf6N2E2_646 are separated by 110 nucleotides Pf6N2E2_646 and Pf6N2E2_647 are separated by 147 nucleotides Pf6N2E2_647 and Pf6N2E2_648 are separated by 47 nucleotides Pf6N2E2_648 and Pf6N2E2_649 overlap by 4 nucleotides
Pf6N2E2_645 - General secretion pathway protein C, at 735,146 to 735,589
_645
Pf6N2E2_646 - General secretion pathway protein I, at 735,700 to 736,074
_646
Pf6N2E2_647 - General secretion pathway protein G, at 736,222 to 736,671
_647
Pf6N2E2_648 - General secretion pathway protein K, at 736,719 to 737,666
_648
Pf6N2E2_649 - General secretion pathway protein L, at 737,663 to 738,835
_649
Position (kb) 736
737 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 735.459 kb on + strand, within Pf6N2E2_645 at 736.159 kb on + strand at 736.171 kb on - strand at 736.171 kb on - strand at 736.171 kb on - strand at 736.519 kb on + strand, within Pf6N2E2_647 at 736.559 kb on - strand, within Pf6N2E2_647 at 736.564 kb on + strand, within Pf6N2E2_647 at 736.565 kb on - strand, within Pf6N2E2_647 at 736.670 kb on - strand at 736.670 kb on - strand at 737.400 kb on + strand, within Pf6N2E2_648 at 737.468 kb on + strand, within Pf6N2E2_648 at 737.618 kb on + strand at 737.662 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction selenate 0.5 M remove 735,459 + Pf6N2E2_645 0.70 -3.1 736,159 + +2.3 736,171 - +0.1 736,171 - +0.2 736,171 - -1.2 736,519 + Pf6N2E2_647 0.66 -0.4 736,559 - Pf6N2E2_647 0.75 -0.9 736,564 + Pf6N2E2_647 0.76 -0.8 736,565 - Pf6N2E2_647 0.76 -0.5 736,670 - -0.9 736,670 - +1.3 737,400 + Pf6N2E2_648 0.72 -1.4 737,468 + Pf6N2E2_648 0.79 -0.3 737,618 + +0.3 737,662 + +0.4
Or see this region's nucleotide sequence