Experiment: selenate 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3738 and Pf6N2E2_3739 overlap by 4 nucleotides Pf6N2E2_3739 and Pf6N2E2_3740 overlap by 4 nucleotides Pf6N2E2_3740 and Pf6N2E2_3741 overlap by 8 nucleotides Pf6N2E2_3741 and Pf6N2E2_3742 overlap by 8 nucleotides
Pf6N2E2_3738 - General secretion pathway protein L, at 4,354,377 to 4,355,480
_3738
Pf6N2E2_3739 - General secretion pathway protein K, at 4,355,477 to 4,356,325
_3739
Pf6N2E2_3740 - General secretion pathway protein J, at 4,356,322 to 4,356,855
_3740
Pf6N2E2_3741 - General secretion pathway protein I, at 4,356,848 to 4,357,234
_3741
Pf6N2E2_3742 - General secretion pathway protein H, at 4,357,227 to 4,357,694
_3742
Position (kb) 4355
4356
4357 Strain fitness (log2 ratio) -1
0
1
2 at 4354.727 kb on - strand, within Pf6N2E2_3738 at 4354.727 kb on - strand, within Pf6N2E2_3738 at 4354.791 kb on + strand, within Pf6N2E2_3738 at 4354.792 kb on - strand, within Pf6N2E2_3738 at 4355.100 kb on + strand, within Pf6N2E2_3738 at 4355.100 kb on + strand, within Pf6N2E2_3738 at 4355.464 kb on + strand at 4355.798 kb on - strand, within Pf6N2E2_3739 at 4355.933 kb on + strand, within Pf6N2E2_3739 at 4356.010 kb on + strand, within Pf6N2E2_3739 at 4356.010 kb on + strand, within Pf6N2E2_3739 at 4356.011 kb on - strand, within Pf6N2E2_3739 at 4356.110 kb on + strand, within Pf6N2E2_3739 at 4356.111 kb on - strand, within Pf6N2E2_3739 at 4356.128 kb on + strand, within Pf6N2E2_3739 at 4356.129 kb on - strand, within Pf6N2E2_3739 at 4356.143 kb on + strand, within Pf6N2E2_3739 at 4356.144 kb on - strand, within Pf6N2E2_3739 at 4356.144 kb on - strand, within Pf6N2E2_3739 at 4356.455 kb on - strand, within Pf6N2E2_3740 at 4356.455 kb on - strand, within Pf6N2E2_3740 at 4356.455 kb on - strand, within Pf6N2E2_3740 at 4356.475 kb on + strand, within Pf6N2E2_3740 at 4356.475 kb on + strand, within Pf6N2E2_3740 at 4356.475 kb on + strand, within Pf6N2E2_3740 at 4356.476 kb on - strand, within Pf6N2E2_3740 at 4356.476 kb on - strand, within Pf6N2E2_3740 at 4356.615 kb on + strand, within Pf6N2E2_3740 at 4356.616 kb on - strand, within Pf6N2E2_3740 at 4356.849 kb on + strand at 4356.849 kb on + strand at 4356.849 kb on + strand at 4356.850 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction selenate 0.5 M remove 4,354,727 - Pf6N2E2_3738 0.32 +1.4 4,354,727 - Pf6N2E2_3738 0.32 -1.1 4,354,791 + Pf6N2E2_3738 0.38 +0.1 4,354,792 - Pf6N2E2_3738 0.38 +0.8 4,355,100 + Pf6N2E2_3738 0.65 -0.1 4,355,100 + Pf6N2E2_3738 0.65 -1.0 4,355,464 + +0.4 4,355,798 - Pf6N2E2_3739 0.38 +0.2 4,355,933 + Pf6N2E2_3739 0.54 +2.6 4,356,010 + Pf6N2E2_3739 0.63 +1.0 4,356,010 + Pf6N2E2_3739 0.63 -0.1 4,356,011 - Pf6N2E2_3739 0.63 +0.2 4,356,110 + Pf6N2E2_3739 0.75 -0.3 4,356,111 - Pf6N2E2_3739 0.75 -0.6 4,356,128 + Pf6N2E2_3739 0.77 +0.9 4,356,129 - Pf6N2E2_3739 0.77 +0.9 4,356,143 + Pf6N2E2_3739 0.78 +0.9 4,356,144 - Pf6N2E2_3739 0.79 +1.0 4,356,144 - Pf6N2E2_3739 0.79 +0.0 4,356,455 - Pf6N2E2_3740 0.25 -0.2 4,356,455 - Pf6N2E2_3740 0.25 +0.9 4,356,455 - Pf6N2E2_3740 0.25 -0.7 4,356,475 + Pf6N2E2_3740 0.29 -0.3 4,356,475 + Pf6N2E2_3740 0.29 +0.4 4,356,475 + Pf6N2E2_3740 0.29 +0.1 4,356,476 - Pf6N2E2_3740 0.29 -0.0 4,356,476 - Pf6N2E2_3740 0.29 -0.4 4,356,615 + Pf6N2E2_3740 0.55 -0.0 4,356,616 - Pf6N2E2_3740 0.55 -0.1 4,356,849 + -0.3 4,356,849 + +1.1 4,356,849 + -0.1 4,356,850 - +0.6
Or see this region's nucleotide sequence