Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10710 and BWI76_RS10715 are separated by 171 nucleotides BWI76_RS10715 and BWI76_RS10720 overlap by 11 nucleotides BWI76_RS10720 and BWI76_RS10725 are separated by 516 nucleotides BWI76_RS10725 and BWI76_RS10730 are separated by 139 nucleotides
BWI76_RS10710: BWI76_RS10710 - Glutamate--putrescine ligase (EC 6.3.1.11) (from data) , at 2,263,423 to 2,264,841
_RS10710
BWI76_RS10715: BWI76_RS10715 - hypothetical protein, at 2,265,013 to 2,265,201
_RS10715
BWI76_RS10720: BWI76_RS10720 - putrescine importer, at 2,265,191 to 2,266,582
_RS10720
BWI76_RS10725: BWI76_RS10725 - hypothetical protein, at 2,267,099 to 2,267,323
_RS10725
BWI76_RS10730: BWI76_RS10730 - bifunctional glucose-1-phosphatase/inositol phosphatase, at 2,267,463 to 2,268,710
_RS10730
Position (kb)
2265
2266
2267 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2264.266 kb on + strand, within BWI76_RS10710 at 2264.645 kb on + strand, within BWI76_RS10710 at 2264.650 kb on + strand, within BWI76_RS10710 at 2264.949 kb on + strand at 2264.949 kb on + strand at 2264.990 kb on - strand at 2264.994 kb on + strand at 2265.219 kb on + strand at 2265.219 kb on + strand at 2265.260 kb on - strand at 2265.446 kb on + strand, within BWI76_RS10720 at 2265.522 kb on - strand, within BWI76_RS10720 at 2265.582 kb on - strand, within BWI76_RS10720 at 2265.602 kb on - strand, within BWI76_RS10720 at 2266.112 kb on - strand, within BWI76_RS10720 at 2266.492 kb on - strand at 2266.506 kb on - strand at 2266.551 kb on - strand at 2266.567 kb on + strand at 2266.567 kb on + strand at 2266.570 kb on - strand at 2266.598 kb on + strand at 2266.739 kb on - strand at 2266.776 kb on + strand at 2266.776 kb on + strand at 2267.058 kb on + strand at 2267.064 kb on + strand at 2267.064 kb on + strand at 2267.066 kb on - strand at 2267.148 kb on - strand, within BWI76_RS10725 at 2267.264 kb on - strand, within BWI76_RS10725 at 2267.502 kb on + strand at 2267.510 kb on - strand at 2267.535 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 2,264,266 + BWI76_RS10710 0.59 -3.1 2,264,645 + BWI76_RS10710 0.86 -3.0 2,264,650 + BWI76_RS10710 0.86 -3.4 2,264,949 + -2.7 2,264,949 + -3.0 2,264,990 - -1.9 2,264,994 + -2.5 2,265,219 + -0.9 2,265,219 + -2.7 2,265,260 - -1.2 2,265,446 + BWI76_RS10720 0.18 -2.8 2,265,522 - BWI76_RS10720 0.24 -1.1 2,265,582 - BWI76_RS10720 0.28 -3.3 2,265,602 - BWI76_RS10720 0.30 -3.3 2,266,112 - BWI76_RS10720 0.66 -3.0 2,266,492 - -1.1 2,266,506 - -1.8 2,266,551 - -2.7 2,266,567 + -1.1 2,266,567 + -0.1 2,266,570 - -3.1 2,266,598 + -1.4 2,266,739 - -1.5 2,266,776 + +0.4 2,266,776 + +4.7 2,267,058 + -0.7 2,267,064 + +0.2 2,267,064 + +1.2 2,267,066 - -1.0 2,267,148 - BWI76_RS10725 0.22 -0.4 2,267,264 - BWI76_RS10725 0.73 +0.1 2,267,502 + +0.7 2,267,510 - +0.2 2,267,535 - +1.5
Or see this region's nucleotide sequence