Experiment: L-Methionine as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgB and DvMF_2285 overlap by 1 nucleotides DvMF_2285 and DvMF_2286 are separated by 19 nucleotides DvMF_2286 and DvMF_2287 are separated by 316 nucleotides
DvMF_2284: flgB - flagellar basal body rod protein FlgB (RefSeq), at 2,873,035 to 2,873,445
flgB
DvMF_2285: DvMF_2285 - flagellar basal-body rod protein FlgC (RefSeq), at 2,873,445 to 2,873,882
_2285
DvMF_2286: DvMF_2286 - flagellar hook-basal body complex subunit FliE (RefSeq), at 2,873,902 to 2,874,234
_2286
DvMF_2287: DvMF_2287 - flagellar M-ring protein FliF (RefSeq), at 2,874,551 to 2,876,170
_2287
Position (kb)
2873
2874
2875 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2873.064 kb on - strand at 2873.236 kb on + strand, within flgB at 2873.403 kb on + strand, within flgB at 2873.566 kb on - strand, within DvMF_2285 at 2873.566 kb on - strand, within DvMF_2285 at 2873.817 kb on - strand, within DvMF_2285 at 2874.020 kb on - strand, within DvMF_2286 at 2874.219 kb on - strand at 2874.503 kb on + strand at 2874.506 kb on - strand at 2874.508 kb on + strand at 2874.583 kb on + strand at 2874.643 kb on - strand at 2874.837 kb on + strand, within DvMF_2287 at 2874.877 kb on - strand, within DvMF_2287 at 2874.909 kb on - strand, within DvMF_2287 at 2874.909 kb on - strand, within DvMF_2287 at 2875.111 kb on - strand, within DvMF_2287 at 2875.170 kb on + strand, within DvMF_2287 at 2875.194 kb on + strand, within DvMF_2287 at 2875.206 kb on - strand, within DvMF_2287
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine as nutrient remove 2,873,064 - +1.5 2,873,236 + flgB DvMF_2284 0.49 +1.7 2,873,403 + flgB DvMF_2284 0.90 +2.1 2,873,566 - DvMF_2285 0.28 +1.8 2,873,566 - DvMF_2285 0.28 +1.3 2,873,817 - DvMF_2285 0.85 +2.5 2,874,020 - DvMF_2286 0.35 +2.2 2,874,219 - +1.9 2,874,503 + +0.1 2,874,506 - +0.0 2,874,508 + +0.6 2,874,583 + +1.9 2,874,643 - +2.1 2,874,837 + DvMF_2287 0.18 +1.8 2,874,877 - DvMF_2287 0.20 +1.7 2,874,909 - DvMF_2287 0.22 +1.7 2,874,909 - DvMF_2287 0.22 +2.6 2,875,111 - DvMF_2287 0.35 +2.2 2,875,170 + DvMF_2287 0.38 +1.5 2,875,194 + DvMF_2287 0.40 +2.1 2,875,206 - DvMF_2287 0.40 +3.0
Or see this region's nucleotide sequence