Experiment: L-Methionine as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0162 and DvMF_0163 are separated by 335 nucleotides DvMF_0163 and DvMF_0164 are separated by 81 nucleotides DvMF_0164 and DvMF_0165 are separated by 121 nucleotides DvMF_0165 and DvMF_0166 are separated by 11 nucleotides
DvMF_0162: DvMF_0162 - hypothetical protein (RefSeq), at 176,913 to 178,202
_0162
DvMF_0163: DvMF_0163 - no description, at 178,538 to 178,755
_0163
DvMF_0164: DvMF_0164 - hypothetical protein (RefSeq), at 178,837 to 179,658
_0164
DvMF_0165: DvMF_0165 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 179,780 to 180,337
_0165
DvMF_0166: DvMF_0166 - Formate dehydrogenase (RefSeq), at 180,349 to 182,484
_0166
Position (kb) 178
179
180 Strain fitness (log2 ratio) -1
0
1 at 177.871 kb on - strand, within DvMF_0162 at 177.940 kb on + strand, within DvMF_0162 at 177.948 kb on - strand, within DvMF_0162 at 177.976 kb on + strand, within DvMF_0162 at 177.984 kb on - strand, within DvMF_0162 at 177.984 kb on - strand, within DvMF_0162 at 178.104 kb on - strand at 178.291 kb on - strand at 178.319 kb on + strand at 178.415 kb on + strand at 178.591 kb on + strand, within DvMF_0163 at 178.591 kb on + strand, within DvMF_0163 at 178.591 kb on + strand, within DvMF_0163 at 178.599 kb on - strand, within DvMF_0163 at 178.788 kb on + strand at 178.796 kb on - strand at 178.796 kb on - strand at 178.833 kb on - strand at 178.853 kb on - strand at 178.882 kb on - strand at 178.962 kb on + strand, within DvMF_0164 at 179.071 kb on - strand, within DvMF_0164 at 179.499 kb on + strand, within DvMF_0164 at 179.523 kb on + strand, within DvMF_0164 at 180.066 kb on + strand, within DvMF_0165 at 180.110 kb on - strand, within DvMF_0165 at 180.407 kb on - strand at 180.502 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine as nutrient remove 177,871 - DvMF_0162 0.74 +0.6 177,940 + DvMF_0162 0.80 +0.9 177,948 - DvMF_0162 0.80 +1.1 177,976 + DvMF_0162 0.82 +1.6 177,984 - DvMF_0162 0.83 +0.6 177,984 - DvMF_0162 0.83 +0.1 178,104 - -0.1 178,291 - +0.1 178,319 + -1.0 178,415 + +0.7 178,591 + DvMF_0163 0.24 +0.4 178,591 + DvMF_0163 0.24 +0.2 178,591 + DvMF_0163 0.24 +0.1 178,599 - DvMF_0163 0.28 +0.6 178,788 + +0.3 178,796 - +0.3 178,796 - -0.0 178,833 - +1.2 178,853 - +0.4 178,882 - +0.6 178,962 + DvMF_0164 0.15 +1.2 179,071 - DvMF_0164 0.28 +0.2 179,499 + DvMF_0164 0.81 +0.7 179,523 + DvMF_0164 0.83 +0.8 180,066 + DvMF_0165 0.51 +0.2 180,110 - DvMF_0165 0.59 -1.0 180,407 - -0.5 180,502 + +0.0
Or see this region's nucleotide sequence