Experiment: L-Methionine as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0150 and DvMF_0151 overlap by 14 nucleotides DvMF_0151 and DvMF_0152 are separated by 29 nucleotides DvMF_0152 and DvMF_0153 are separated by 0 nucleotides DvMF_0153 and ilvH are separated by 12 nucleotides ilvH and DvMF_0155 are separated by 335 nucleotides
DvMF_0150: DvMF_0150 - DivIVA family protein (RefSeq), at 166,177 to 166,686
_0150
DvMF_0151: DvMF_0151 - protein of unknown function DUF167 (RefSeq), at 166,673 to 166,993
_0151
DvMF_0152: DvMF_0152 - hypothetical protein (RefSeq), at 167,023 to 167,253
_0152
DvMF_0153: DvMF_0153 - acetolactate synthase, large subunit, biosynthetic type (RefSeq), at 167,254 to 168,942
_0153
DvMF_0154: ilvH - acetolactate synthase 3 regulatory subunit (RefSeq), at 168,955 to 169,446
ilvH
DvMF_0155: DvMF_0155 - ketol-acid reductoisomerase (RefSeq), at 169,782 to 170,774
_0155
Position (kb) 167
168
169 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 166.452 kb on - strand, within DvMF_0150 at 166.500 kb on + strand, within DvMF_0150 at 166.630 kb on + strand, within DvMF_0150 at 166.823 kb on + strand, within DvMF_0151 at 167.247 kb on - strand at 167.302 kb on + strand at 167.442 kb on + strand, within DvMF_0153 at 167.476 kb on - strand, within DvMF_0153 at 168.461 kb on - strand, within DvMF_0153 at 168.461 kb on - strand, within DvMF_0153 at 168.549 kb on - strand, within DvMF_0153 at 168.944 kb on - strand at 169.002 kb on + strand at 169.344 kb on + strand, within ilvH at 169.429 kb on - strand at 169.802 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine as nutrient remove 166,452 - DvMF_0150 0.54 -0.2 166,500 + DvMF_0150 0.63 +0.4 166,630 + DvMF_0150 0.89 +0.6 166,823 + DvMF_0151 0.47 +0.2 167,247 - -0.7 167,302 + -3.2 167,442 + DvMF_0153 0.11 -2.8 167,476 - DvMF_0153 0.13 -2.6 168,461 - DvMF_0153 0.71 -2.9 168,461 - DvMF_0153 0.71 -2.8 168,549 - DvMF_0153 0.77 -3.5 168,944 - +0.2 169,002 + -0.7 169,344 + ilvH DvMF_0154 0.79 -0.7 169,429 - -0.0 169,802 + -1.3
Or see this region's nucleotide sequence