Experiment: m.b. Vancomycin 0.064 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0089 and Dshi_0090 overlap by 4 nucleotides Dshi_0090 and Dshi_0091 are separated by 7 nucleotides
Dshi_0089: Dshi_0089 - phosphoglycerate mutase, 2,3-bisphosphoglycerate-independent (RefSeq), at 87,158 to 88,675
_0089
Dshi_0090: Dshi_0090 - peptidase M23B (RefSeq), at 88,672 to 89,775
_0090
Dshi_0091: Dshi_0091 - carboxyl-terminal protease (RefSeq), at 89,783 to 91,120
_0091
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 87.685 kb on + strand, within Dshi_0089 at 87.706 kb on + strand, within Dshi_0089 at 87.786 kb on - strand, within Dshi_0089 at 87.877 kb on - strand, within Dshi_0089 at 88.045 kb on - strand, within Dshi_0089 at 88.239 kb on + strand, within Dshi_0089 at 88.387 kb on + strand, within Dshi_0089 at 88.509 kb on - strand, within Dshi_0089 at 88.529 kb on - strand at 88.560 kb on + strand at 88.656 kb on + strand at 88.833 kb on - strand, within Dshi_0090 at 88.859 kb on + strand, within Dshi_0090 at 88.872 kb on + strand, within Dshi_0090 at 88.916 kb on + strand, within Dshi_0090 at 88.924 kb on - strand, within Dshi_0090 at 89.036 kb on + strand, within Dshi_0090 at 89.257 kb on - strand, within Dshi_0090 at 89.369 kb on - strand, within Dshi_0090 at 89.376 kb on - strand, within Dshi_0090 at 89.376 kb on - strand, within Dshi_0090 at 89.526 kb on - strand, within Dshi_0090 at 89.526 kb on - strand, within Dshi_0090 at 89.583 kb on + strand, within Dshi_0090 at 89.586 kb on + strand, within Dshi_0090 at 89.605 kb on - strand, within Dshi_0090 at 89.643 kb on + strand, within Dshi_0090 at 89.643 kb on + strand, within Dshi_0090 at 89.651 kb on - strand, within Dshi_0090 at 89.708 kb on + strand at 89.726 kb on + strand at 89.754 kb on + strand at 89.772 kb on - strand at 89.901 kb on + strand at 89.901 kb on + strand at 89.909 kb on - strand at 89.930 kb on - strand, within Dshi_0091 at 89.999 kb on - strand, within Dshi_0091 at 90.230 kb on - strand, within Dshi_0091 at 90.244 kb on + strand, within Dshi_0091 at 90.293 kb on + strand, within Dshi_0091 at 90.341 kb on - strand, within Dshi_0091 at 90.412 kb on - strand, within Dshi_0091 at 90.607 kb on + strand, within Dshi_0091 at 90.615 kb on - strand, within Dshi_0091 at 90.768 kb on + strand, within Dshi_0091
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Vancomycin 0.064 mg/ml remove 87,685 + Dshi_0089 0.35 -0.4 87,706 + Dshi_0089 0.36 -1.7 87,786 - Dshi_0089 0.41 -1.4 87,877 - Dshi_0089 0.47 +0.1 88,045 - Dshi_0089 0.58 -0.9 88,239 + Dshi_0089 0.71 -0.9 88,387 + Dshi_0089 0.81 -0.9 88,509 - Dshi_0089 0.89 -1.4 88,529 - -1.9 88,560 + -2.0 88,656 + -2.2 88,833 - Dshi_0090 0.15 -0.2 88,859 + Dshi_0090 0.17 -0.8 88,872 + Dshi_0090 0.18 -0.2 88,916 + Dshi_0090 0.22 -0.9 88,924 - Dshi_0090 0.23 -0.6 89,036 + Dshi_0090 0.33 +0.4 89,257 - Dshi_0090 0.53 -0.4 89,369 - Dshi_0090 0.63 +0.6 89,376 - Dshi_0090 0.64 -0.4 89,376 - Dshi_0090 0.64 +1.1 89,526 - Dshi_0090 0.77 -0.1 89,526 - Dshi_0090 0.77 -0.6 89,583 + Dshi_0090 0.83 -1.1 89,586 + Dshi_0090 0.83 -0.1 89,605 - Dshi_0090 0.85 +0.1 89,643 + Dshi_0090 0.88 -2.4 89,643 + Dshi_0090 0.88 -0.3 89,651 - Dshi_0090 0.89 -0.5 89,708 + -0.5 89,726 + -0.5 89,754 + -0.9 89,772 - -0.7 89,901 + +0.1 89,901 + -2.7 89,909 - +1.1 89,930 - Dshi_0091 0.11 +0.1 89,999 - Dshi_0091 0.16 -1.4 90,230 - Dshi_0091 0.33 -1.6 90,244 + Dshi_0091 0.34 -0.2 90,293 + Dshi_0091 0.38 -2.0 90,341 - Dshi_0091 0.42 -2.0 90,412 - Dshi_0091 0.47 -0.4 90,607 + Dshi_0091 0.62 -0.6 90,615 - Dshi_0091 0.62 -3.2 90,768 + Dshi_0091 0.74 -2.1
Or see this region's nucleotide sequence