Experiment: MoYLS4 with peroxynitrite 200uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ftsH and folP overlap by 4 nucleotides folP and DVU1280 are separated by 8 nucleotides DVU1280 and DVU1281 are separated by 1 nucleotides DVU1281 and glmM are separated by 59 nucleotides
DVU1278: ftsH - cell division protein FtsH (TIGR), at 1,369,986 to 1,371,956
ftsH
DVU1279: folP - dihydropteroate synthase (TIGR), at 1,371,953 to 1,372,813
folP
DVU1280: DVU1280 - conserved hypothetical protein TIGR00159 (TIGR), at 1,372,822 to 1,373,568
DVU1280
DVU1281: DVU1281 - conserved hypothetical protein (TIGR), at 1,373,570 to 1,374,472
DVU1281
DVU1282: glmM - phosphoglucosamine mutase (TIGR), at 1,374,532 to 1,375,884
glmM
Position (kb)
1372
1373
1374 Strain fitness (log2 ratio)
-2
-1
0
1 at 1371.911 kb on - strand at 1372.780 kb on - strand at 1372.780 kb on - strand at 1372.780 kb on - strand at 1372.780 kb on - strand at 1372.999 kb on + strand, within DVU1280 at 1373.169 kb on - strand, within DVU1280 at 1373.348 kb on - strand, within DVU1280 at 1373.419 kb on + strand, within DVU1280 at 1373.524 kb on - strand at 1373.816 kb on + strand, within DVU1281 at 1373.841 kb on - strand, within DVU1281 at 1374.004 kb on - strand, within DVU1281 at 1374.026 kb on - strand, within DVU1281 at 1374.135 kb on + strand, within DVU1281 at 1374.166 kb on + strand, within DVU1281 at 1374.261 kb on - strand, within DVU1281 at 1374.293 kb on - strand, within DVU1281 at 1374.315 kb on + strand, within DVU1281 at 1374.325 kb on + strand, within DVU1281 at 1374.331 kb on - strand, within DVU1281 at 1374.345 kb on + strand, within DVU1281 at 1374.363 kb on + strand, within DVU1281 at 1374.409 kb on - strand at 1374.428 kb on - strand at 1374.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with peroxynitrite 200uM remove 1,371,911 - -0.5 1,372,780 - -0.6 1,372,780 - -0.3 1,372,780 - -0.4 1,372,780 - -0.2 1,372,999 + DVU1280 0.24 -1.5 1,373,169 - DVU1280 0.46 -0.4 1,373,348 - DVU1280 0.70 -1.1 1,373,419 + DVU1280 0.80 -2.4 1,373,524 - +0.5 1,373,816 + DVU1281 0.27 +0.4 1,373,841 - DVU1281 0.30 -0.6 1,374,004 - DVU1281 0.48 +0.8 1,374,026 - DVU1281 0.50 -1.0 1,374,135 + DVU1281 0.63 -1.2 1,374,166 + DVU1281 0.66 -1.4 1,374,261 - DVU1281 0.77 -0.7 1,374,293 - DVU1281 0.80 -1.1 1,374,315 + DVU1281 0.83 -1.1 1,374,325 + DVU1281 0.84 -1.4 1,374,331 - DVU1281 0.84 -0.9 1,374,345 + DVU1281 0.86 -0.2 1,374,363 + DVU1281 0.88 +1.7 1,374,409 - -0.2 1,374,428 - +0.8 1,374,444 - -0.9
Or see this region's nucleotide sequence