Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05927 and H281DRAFT_05928 are separated by 410 nucleotides H281DRAFT_05928 and H281DRAFT_05929 are separated by 25 nucleotides
H281DRAFT_05927: H281DRAFT_05927 - Glycogen debranching enzyme (alpha-1,6-glucosidase), at 62,841 to 65,123
_05927
H281DRAFT_05928: H281DRAFT_05928 - hypothetical protein, at 65,534 to 65,935
_05928
H281DRAFT_05929: H281DRAFT_05929 - Pimeloyl-ACP methyl ester carboxylesterase, at 65,961 to 66,749
_05929
Position (kb) 65
66 Strain fitness (log2 ratio) -1
0
1
2 at 64.552 kb on + strand, within H281DRAFT_05927 at 65.137 kb on - strand at 65.204 kb on - strand at 65.411 kb on + strand at 65.727 kb on + strand, within H281DRAFT_05928 at 65.784 kb on - strand, within H281DRAFT_05928 at 65.934 kb on - strand at 65.934 kb on - strand at 65.999 kb on - strand at 66.182 kb on - strand, within H281DRAFT_05929 at 66.444 kb on + strand, within H281DRAFT_05929 at 66.444 kb on + strand, within H281DRAFT_05929 at 66.445 kb on - strand, within H281DRAFT_05929 at 66.445 kb on - strand, within H281DRAFT_05929 at 66.644 kb on - strand, within H281DRAFT_05929 at 66.742 kb on + strand at 66.789 kb on - strand at 66.910 kb on + strand at 66.910 kb on + strand at 66.910 kb on + strand at 66.910 kb on + strand at 66.911 kb on - strand at 66.911 kb on - strand at 66.911 kb on - strand at 66.920 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 64,552 + H281DRAFT_05927 0.75 -0.2 65,137 - -0.6 65,204 - +0.1 65,411 + +1.4 65,727 + H281DRAFT_05928 0.48 -0.1 65,784 - H281DRAFT_05928 0.62 +1.1 65,934 - +0.1 65,934 - +0.2 65,999 - +0.6 66,182 - H281DRAFT_05929 0.28 -0.3 66,444 + H281DRAFT_05929 0.61 -0.2 66,444 + H281DRAFT_05929 0.61 -0.3 66,445 - H281DRAFT_05929 0.61 -0.0 66,445 - H281DRAFT_05929 0.61 +0.1 66,644 - H281DRAFT_05929 0.87 -0.1 66,742 + -0.4 66,789 - +0.9 66,910 + +0.5 66,910 + +0.1 66,910 + -1.6 66,910 + +0.0 66,911 - -0.6 66,911 - -0.9 66,911 - -0.0 66,920 + +1.8
Or see this region's nucleotide sequence