Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05380 and H281DRAFT_05381 are separated by 10 nucleotides H281DRAFT_05381 and H281DRAFT_05382 are separated by 121 nucleotides H281DRAFT_05382 and H281DRAFT_05383 are separated by 183 nucleotides
H281DRAFT_05380: H281DRAFT_05380 - transcriptional regulator, AraC family, at 138,826 to 140,028
_05380
H281DRAFT_05381: H281DRAFT_05381 - phosphonoacetate hydrolase, at 140,039 to 140,347
_05381
H281DRAFT_05382: H281DRAFT_05382 - diguanylate cyclase (GGDEF) domain-containing protein, at 140,469 to 141,947
_05382
H281DRAFT_05383: H281DRAFT_05383 - D-3-phosphoglycerate dehydrogenase, at 142,131 to 143,063
_05383
Position (kb)
140
141
142 Strain fitness (log2 ratio)
-1
0
1 at 139.795 kb on + strand, within H281DRAFT_05380 at 139.883 kb on - strand, within H281DRAFT_05380 at 139.969 kb on + strand at 140.056 kb on - strand at 140.136 kb on - strand, within H281DRAFT_05381 at 140.317 kb on - strand at 140.317 kb on - strand at 140.911 kb on + strand, within H281DRAFT_05382 at 141.428 kb on - strand, within H281DRAFT_05382 at 141.938 kb on + strand at 141.939 kb on - strand at 141.980 kb on - strand at 141.980 kb on - strand at 142.054 kb on + strand at 142.055 kb on - strand at 142.055 kb on - strand at 142.897 kb on - strand, within H281DRAFT_05383 at 142.897 kb on - strand, within H281DRAFT_05383
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 139,795 + H281DRAFT_05380 0.81 +0.5 139,883 - H281DRAFT_05380 0.88 -0.3 139,969 + +0.0 140,056 - -0.4 140,136 - H281DRAFT_05381 0.31 -0.4 140,317 - +0.2 140,317 - +0.5 140,911 + H281DRAFT_05382 0.30 +0.4 141,428 - H281DRAFT_05382 0.65 +0.7 141,938 + -1.2 141,939 - +0.3 141,980 - -0.7 141,980 - +0.4 142,054 + +0.2 142,055 - +0.0 142,055 - +0.0 142,897 - H281DRAFT_05383 0.82 +0.6 142,897 - H281DRAFT_05383 0.82 -0.1
Or see this region's nucleotide sequence