Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04497 and H281DRAFT_04498 are separated by 80 nucleotides H281DRAFT_04498 and H281DRAFT_04499 are separated by 69 nucleotides H281DRAFT_04499 and H281DRAFT_04500 are separated by 138 nucleotides H281DRAFT_04500 and H281DRAFT_04501 are separated by 251 nucleotides
H281DRAFT_04497: H281DRAFT_04497 - Pimeloyl-ACP methyl ester carboxylesterase, at 229,545 to 230,456
_04497
H281DRAFT_04498: H281DRAFT_04498 - Enamine deaminase RidA, house cleaning of reactive enamine intermediates, YjgF/YER057c/UK114 family, at 230,537 to 230,890
_04498
H281DRAFT_04499: H281DRAFT_04499 - GTP pyrophosphokinase, at 230,960 to 233,203
_04499
H281DRAFT_04500: H281DRAFT_04500 - tRNA_Val_GAC, at 233,342 to 233,418
_04500
H281DRAFT_04501: H281DRAFT_04501 - Ser-tRNA(Thr) hydrolase /threonyl-tRNA synthetase, at 233,670 to 235,577
_04501
Position (kb) 230
231
232
233
234 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 229.961 kb on - strand, within H281DRAFT_04497 at 229.961 kb on - strand, within H281DRAFT_04497 at 230.124 kb on - strand, within H281DRAFT_04497 at 230.201 kb on + strand, within H281DRAFT_04497 at 230.236 kb on + strand, within H281DRAFT_04497 at 230.454 kb on + strand at 230.455 kb on - strand at 230.455 kb on - strand at 230.457 kb on + strand at 230.457 kb on + strand at 230.458 kb on - strand at 230.458 kb on - strand at 230.458 kb on - strand at 230.517 kb on + strand at 230.517 kb on + strand at 230.517 kb on + strand at 230.518 kb on - strand at 230.518 kb on - strand at 230.522 kb on - strand at 230.604 kb on - strand, within H281DRAFT_04498 at 230.707 kb on + strand, within H281DRAFT_04498 at 230.735 kb on + strand, within H281DRAFT_04498 at 230.736 kb on - strand, within H281DRAFT_04498 at 230.736 kb on - strand, within H281DRAFT_04498 at 230.736 kb on - strand, within H281DRAFT_04498 at 230.736 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 231.384 kb on + strand, within H281DRAFT_04499 at 231.394 kb on - strand, within H281DRAFT_04499 at 231.429 kb on - strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.486 kb on - strand, within H281DRAFT_04499 at 231.486 kb on - strand, within H281DRAFT_04499 at 231.486 kb on - strand, within H281DRAFT_04499 at 231.572 kb on + strand, within H281DRAFT_04499 at 231.572 kb on + strand, within H281DRAFT_04499 at 231.572 kb on + strand, within H281DRAFT_04499 at 231.573 kb on - strand, within H281DRAFT_04499 at 231.573 kb on - strand, within H281DRAFT_04499 at 231.573 kb on - strand, within H281DRAFT_04499 at 231.573 kb on - strand, within H281DRAFT_04499 at 231.573 kb on - strand, within H281DRAFT_04499 at 231.573 kb on - strand, within H281DRAFT_04499 at 231.573 kb on - strand, within H281DRAFT_04499 at 231.574 kb on + strand, within H281DRAFT_04499 at 231.575 kb on - strand, within H281DRAFT_04499 at 231.575 kb on - strand, within H281DRAFT_04499 at 231.623 kb on + strand, within H281DRAFT_04499 at 231.623 kb on + strand, within H281DRAFT_04499 at 231.624 kb on - strand, within H281DRAFT_04499 at 231.624 kb on - strand, within H281DRAFT_04499 at 231.624 kb on - strand, within H281DRAFT_04499 at 231.624 kb on - strand, within H281DRAFT_04499 at 231.687 kb on + strand, within H281DRAFT_04499 at 231.706 kb on + strand, within H281DRAFT_04499 at 231.707 kb on - strand, within H281DRAFT_04499 at 231.707 kb on - strand, within H281DRAFT_04499 at 231.707 kb on - strand, within H281DRAFT_04499 at 231.707 kb on - strand, within H281DRAFT_04499 at 231.707 kb on - strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.711 kb on - strand, within H281DRAFT_04499 at 231.711 kb on - strand, within H281DRAFT_04499 at 231.711 kb on - strand, within H281DRAFT_04499 at 231.711 kb on - strand, within H281DRAFT_04499 at 231.711 kb on - strand, within H281DRAFT_04499 at 231.711 kb on - strand, within H281DRAFT_04499 at 231.712 kb on + strand, within H281DRAFT_04499 at 231.712 kb on + strand, within H281DRAFT_04499 at 231.712 kb on + strand, within H281DRAFT_04499 at 231.712 kb on + strand, within H281DRAFT_04499 at 231.869 kb on + strand, within H281DRAFT_04499 at 231.870 kb on - strand, within H281DRAFT_04499 at 231.870 kb on - strand, within H281DRAFT_04499 at 231.896 kb on + strand, within H281DRAFT_04499 at 231.897 kb on - strand, within H281DRAFT_04499 at 231.897 kb on - strand, within H281DRAFT_04499 at 231.898 kb on + strand, within H281DRAFT_04499 at 231.898 kb on + strand, within H281DRAFT_04499 at 231.898 kb on + strand, within H281DRAFT_04499 at 231.898 kb on + strand, within H281DRAFT_04499 at 231.911 kb on - strand, within H281DRAFT_04499 at 231.911 kb on - strand, within H281DRAFT_04499 at 231.911 kb on - strand, within H281DRAFT_04499 at 231.911 kb on - strand, within H281DRAFT_04499 at 231.987 kb on - strand, within H281DRAFT_04499 at 232.065 kb on - strand, within H281DRAFT_04499 at 232.065 kb on - strand, within H281DRAFT_04499 at 232.167 kb on + strand, within H281DRAFT_04499 at 232.168 kb on - strand, within H281DRAFT_04499 at 232.199 kb on + strand, within H281DRAFT_04499 at 232.199 kb on + strand, within H281DRAFT_04499 at 232.200 kb on - strand, within H281DRAFT_04499 at 232.200 kb on - strand, within H281DRAFT_04499 at 232.200 kb on - strand, within H281DRAFT_04499 at 232.277 kb on - strand, within H281DRAFT_04499 at 232.433 kb on - strand, within H281DRAFT_04499 at 232.482 kb on + strand, within H281DRAFT_04499 at 232.483 kb on - strand, within H281DRAFT_04499 at 232.483 kb on - strand, within H281DRAFT_04499 at 232.718 kb on - strand, within H281DRAFT_04499 at 233.269 kb on + strand at 233.294 kb on - strand at 233.325 kb on + strand at 233.489 kb on + strand at 233.634 kb on + strand at 233.636 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 229,961 - H281DRAFT_04497 0.46 -0.3 229,961 - H281DRAFT_04497 0.46 +0.4 230,124 - H281DRAFT_04497 0.63 -0.2 230,201 + H281DRAFT_04497 0.72 +0.2 230,236 + H281DRAFT_04497 0.76 +0.3 230,454 + +0.1 230,455 - +0.1 230,455 - +0.2 230,457 + +0.4 230,457 + +0.1 230,458 - +0.2 230,458 - +0.3 230,458 - +0.7 230,517 + +0.6 230,517 + +0.3 230,517 + -0.5 230,518 - -0.6 230,518 - -0.8 230,522 - +0.2 230,604 - H281DRAFT_04498 0.19 +0.4 230,707 + H281DRAFT_04498 0.48 +0.7 230,735 + H281DRAFT_04498 0.56 +0.5 230,736 - H281DRAFT_04498 0.56 -0.8 230,736 - H281DRAFT_04498 0.56 -0.3 230,736 - H281DRAFT_04498 0.56 +0.4 230,736 - H281DRAFT_04498 0.56 -0.1 230,738 - H281DRAFT_04498 0.57 -0.4 230,738 - H281DRAFT_04498 0.57 -0.8 230,738 - H281DRAFT_04498 0.57 -0.6 230,738 - H281DRAFT_04498 0.57 +0.2 230,738 - H281DRAFT_04498 0.57 -1.9 231,384 + H281DRAFT_04499 0.19 -1.0 231,394 - H281DRAFT_04499 0.19 -0.5 231,429 - H281DRAFT_04499 0.21 +0.6 231,485 + H281DRAFT_04499 0.23 -0.0 231,485 + H281DRAFT_04499 0.23 +0.5 231,485 + H281DRAFT_04499 0.23 -0.5 231,485 + H281DRAFT_04499 0.23 -0.7 231,485 + H281DRAFT_04499 0.23 -0.6 231,485 + H281DRAFT_04499 0.23 -0.7 231,485 + H281DRAFT_04499 0.23 +1.0 231,485 + H281DRAFT_04499 0.23 -0.8 231,485 + H281DRAFT_04499 0.23 -0.3 231,486 - H281DRAFT_04499 0.23 -0.4 231,486 - H281DRAFT_04499 0.23 -0.4 231,486 - H281DRAFT_04499 0.23 -0.1 231,572 + H281DRAFT_04499 0.27 -0.7 231,572 + H281DRAFT_04499 0.27 -0.4 231,572 + H281DRAFT_04499 0.27 +0.4 231,573 - H281DRAFT_04499 0.27 -1.0 231,573 - H281DRAFT_04499 0.27 -0.2 231,573 - H281DRAFT_04499 0.27 -0.3 231,573 - H281DRAFT_04499 0.27 -0.5 231,573 - H281DRAFT_04499 0.27 -1.0 231,573 - H281DRAFT_04499 0.27 -0.7 231,573 - H281DRAFT_04499 0.27 -1.0 231,574 + H281DRAFT_04499 0.27 -0.1 231,575 - H281DRAFT_04499 0.27 -0.7 231,575 - H281DRAFT_04499 0.27 -1.2 231,623 + H281DRAFT_04499 0.30 -1.3 231,623 + H281DRAFT_04499 0.30 -1.2 231,624 - H281DRAFT_04499 0.30 -1.3 231,624 - H281DRAFT_04499 0.30 -0.6 231,624 - H281DRAFT_04499 0.30 -0.8 231,624 - H281DRAFT_04499 0.30 -0.5 231,687 + H281DRAFT_04499 0.32 -0.5 231,706 + H281DRAFT_04499 0.33 -0.0 231,707 - H281DRAFT_04499 0.33 -0.6 231,707 - H281DRAFT_04499 0.33 -1.4 231,707 - H281DRAFT_04499 0.33 -0.6 231,707 - H281DRAFT_04499 0.33 -0.1 231,707 - H281DRAFT_04499 0.33 -0.1 231,710 + H281DRAFT_04499 0.33 -0.8 231,710 + H281DRAFT_04499 0.33 -0.9 231,710 + H281DRAFT_04499 0.33 -0.4 231,710 + H281DRAFT_04499 0.33 +0.0 231,710 + H281DRAFT_04499 0.33 +0.1 231,710 + H281DRAFT_04499 0.33 -0.1 231,710 + H281DRAFT_04499 0.33 -1.0 231,710 + H281DRAFT_04499 0.33 -0.1 231,711 - H281DRAFT_04499 0.33 +0.2 231,711 - H281DRAFT_04499 0.33 -0.5 231,711 - H281DRAFT_04499 0.33 -0.6 231,711 - H281DRAFT_04499 0.33 -0.6 231,711 - H281DRAFT_04499 0.33 -0.9 231,711 - H281DRAFT_04499 0.33 -0.8 231,712 + H281DRAFT_04499 0.34 -0.5 231,712 + H281DRAFT_04499 0.34 -1.2 231,712 + H281DRAFT_04499 0.34 -0.9 231,712 + H281DRAFT_04499 0.34 -1.0 231,869 + H281DRAFT_04499 0.41 +0.2 231,870 - H281DRAFT_04499 0.41 -0.8 231,870 - H281DRAFT_04499 0.41 -1.8 231,896 + H281DRAFT_04499 0.42 -0.4 231,897 - H281DRAFT_04499 0.42 -1.2 231,897 - H281DRAFT_04499 0.42 -1.2 231,898 + H281DRAFT_04499 0.42 +0.6 231,898 + H281DRAFT_04499 0.42 -1.6 231,898 + H281DRAFT_04499 0.42 -0.4 231,898 + H281DRAFT_04499 0.42 -0.7 231,911 - H281DRAFT_04499 0.42 -0.8 231,911 - H281DRAFT_04499 0.42 -0.7 231,911 - H281DRAFT_04499 0.42 -0.2 231,911 - H281DRAFT_04499 0.42 +0.3 231,987 - H281DRAFT_04499 0.46 -0.4 232,065 - H281DRAFT_04499 0.49 -0.7 232,065 - H281DRAFT_04499 0.49 -0.2 232,167 + H281DRAFT_04499 0.54 -0.3 232,168 - H281DRAFT_04499 0.54 -1.5 232,199 + H281DRAFT_04499 0.55 -1.5 232,199 + H281DRAFT_04499 0.55 +0.1 232,200 - H281DRAFT_04499 0.55 -1.0 232,200 - H281DRAFT_04499 0.55 -0.4 232,200 - H281DRAFT_04499 0.55 -0.1 232,277 - H281DRAFT_04499 0.59 -0.5 232,433 - H281DRAFT_04499 0.66 -0.7 232,482 + H281DRAFT_04499 0.68 -1.2 232,483 - H281DRAFT_04499 0.68 +0.4 232,483 - H281DRAFT_04499 0.68 -0.1 232,718 - H281DRAFT_04499 0.78 +0.0 233,269 + +0.5 233,294 - -0.4 233,325 + +0.0 233,489 + -3.6 233,634 + +0.5 233,636 + +0.1
Or see this region's nucleotide sequence