Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04019 and H281DRAFT_04020 are separated by 11 nucleotides H281DRAFT_04020 and H281DRAFT_04021 are separated by 254 nucleotides H281DRAFT_04021 and H281DRAFT_04022 are separated by 56 nucleotides H281DRAFT_04022 and H281DRAFT_04023 overlap by 4 nucleotides
H281DRAFT_04019: H281DRAFT_04019 - diaminohydroxyphosphoribosylaminopyrimidine deaminase, at 121,150 to 122,307
_04019
H281DRAFT_04020: H281DRAFT_04020 - riboflavin synthase alpha chain, at 122,319 to 122,933
_04020
H281DRAFT_04021: H281DRAFT_04021 - 3,4-dihydroxy-2-butanone 4-phosphate synthase /GTP cyclohydrolase II, at 123,188 to 124,318
_04021
H281DRAFT_04022: H281DRAFT_04022 - 6,7-dimethyl-8-ribityllumazine synthase, at 124,375 to 124,878
_04022
H281DRAFT_04023: H281DRAFT_04023 - NusB antitermination factor, at 124,875 to 125,312
_04023
Position (kb) 123
124
125 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 122.312 kb on + strand at 123.075 kb on - strand at 123.075 kb on - strand at 123.075 kb on - strand at 123.115 kb on + strand at 123.115 kb on + strand at 123.116 kb on - strand at 123.155 kb on + strand at 123.205 kb on + strand at 123.205 kb on + strand at 123.205 kb on + strand at 123.428 kb on + strand, within H281DRAFT_04021 at 123.547 kb on + strand, within H281DRAFT_04021 at 123.811 kb on + strand, within H281DRAFT_04021 at 123.863 kb on + strand, within H281DRAFT_04021 at 123.863 kb on + strand, within H281DRAFT_04021 at 123.863 kb on + strand, within H281DRAFT_04021 at 125.310 kb on + strand at 125.311 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 122,312 + +0.6 123,075 - +0.1 123,075 - +0.5 123,075 - +0.3 123,115 + -0.9 123,115 + +0.2 123,116 - +0.5 123,155 + +0.1 123,205 + -2.4 123,205 + -3.6 123,205 + -4.0 123,428 + H281DRAFT_04021 0.21 -2.9 123,547 + H281DRAFT_04021 0.32 -3.2 123,811 + H281DRAFT_04021 0.55 -1.7 123,863 + H281DRAFT_04021 0.60 -0.7 123,863 + H281DRAFT_04021 0.60 -2.0 123,863 + H281DRAFT_04021 0.60 -2.8 125,310 + -0.2 125,311 - -0.1
Or see this region's nucleotide sequence