Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02477 and H281DRAFT_02478 are separated by 79 nucleotides H281DRAFT_02478 and H281DRAFT_02479 are separated by 125 nucleotides
H281DRAFT_02477: H281DRAFT_02477 - glycine betaine/proline transport system substrate-binding protein, at 48,997 to 49,854
_02477
H281DRAFT_02478: H281DRAFT_02478 - formyltetrahydrofolate deformylase (EC 3.5.1.10), at 49,934 to 50,809
_02478
H281DRAFT_02479: H281DRAFT_02479 - transcriptional regulator, LysR family, at 50,935 to 51,849
_02479
Position (kb)
49
50
51 Strain fitness (log2 ratio)
-1
0
1 at 49.062 kb on - strand at 49.852 kb on + strand at 49.852 kb on + strand at 49.865 kb on - strand at 49.936 kb on - strand at 49.936 kb on - strand at 49.967 kb on - strand at 50.520 kb on - strand, within H281DRAFT_02478 at 50.703 kb on + strand, within H281DRAFT_02478 at 50.703 kb on + strand, within H281DRAFT_02478 at 50.704 kb on - strand, within H281DRAFT_02478 at 50.704 kb on - strand, within H281DRAFT_02478 at 50.932 kb on + strand at 50.932 kb on + strand at 50.932 kb on + strand at 50.936 kb on + strand at 50.936 kb on + strand at 50.986 kb on + strand at 51.024 kb on - strand at 51.524 kb on + strand, within H281DRAFT_02479 at 51.525 kb on - strand at 51.734 kb on - strand, within H281DRAFT_02479
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 49,062 - -0.3 49,852 + +0.6 49,852 + +0.1 49,865 - +1.2 49,936 - -0.7 49,936 - +0.3 49,967 - +0.8 50,520 - H281DRAFT_02478 0.67 +1.0 50,703 + H281DRAFT_02478 0.88 +0.1 50,703 + H281DRAFT_02478 0.88 -0.0 50,704 - H281DRAFT_02478 0.88 +0.9 50,704 - H281DRAFT_02478 0.88 +0.3 50,932 + -0.1 50,932 + -0.1 50,932 + -0.2 50,936 + +0.1 50,936 + +0.2 50,986 + -0.1 51,024 - -0.5 51,524 + H281DRAFT_02479 0.64 -0.4 51,525 - -0.8 51,734 - H281DRAFT_02479 0.87 +1.0
Or see this region's nucleotide sequence