Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02409 and H281DRAFT_02410 are separated by 160 nucleotides H281DRAFT_02410 and H281DRAFT_02411 are separated by 78 nucleotides H281DRAFT_02411 and H281DRAFT_02414 are separated by 456 nucleotides H281DRAFT_02414 and H281DRAFT_02415 are separated by 182 nucleotides
H281DRAFT_02409: H281DRAFT_02409 - glycine dehydrogenase, at 580,799 to 583,735
_02409
H281DRAFT_02410: H281DRAFT_02410 - glycine cleavage system H protein, at 583,896 to 584,276
_02410
H281DRAFT_02411: H281DRAFT_02411 - aminomethyltransferase, at 584,355 to 585,473
_02411
H281DRAFT_02414: H281DRAFT_02414 - hypothetical protein, at 585,930 to 586,280
_02414
H281DRAFT_02415: H281DRAFT_02415 - Predicted dehydrogenase, at 586,463 to 587,512
_02415
Position (kb)
584
585
586 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 583.357 kb on - strand, within H281DRAFT_02409 at 583.357 kb on - strand, within H281DRAFT_02409 at 583.357 kb on - strand, within H281DRAFT_02409 at 583.366 kb on - strand, within H281DRAFT_02409 at 583.391 kb on - strand, within H281DRAFT_02409 at 583.422 kb on + strand, within H281DRAFT_02409 at 583.422 kb on + strand, within H281DRAFT_02409 at 583.423 kb on - strand, within H281DRAFT_02409 at 583.423 kb on - strand, within H281DRAFT_02409 at 583.423 kb on - strand, within H281DRAFT_02409 at 583.441 kb on - strand, within H281DRAFT_02409 at 583.441 kb on - strand, within H281DRAFT_02409 at 583.507 kb on + strand at 583.508 kb on - strand at 583.884 kb on + strand at 583.884 kb on + strand at 583.884 kb on + strand at 583.884 kb on + strand at 583.884 kb on + strand at 583.885 kb on - strand at 583.886 kb on + strand at 583.886 kb on + strand at 583.886 kb on + strand at 583.886 kb on + strand at 583.887 kb on - strand at 583.887 kb on - strand at 583.887 kb on - strand at 583.919 kb on - strand at 583.990 kb on + strand, within H281DRAFT_02410 at 583.991 kb on - strand, within H281DRAFT_02410 at 584.068 kb on + strand, within H281DRAFT_02410 at 584.068 kb on + strand, within H281DRAFT_02410 at 584.069 kb on - strand, within H281DRAFT_02410 at 584.069 kb on - strand, within H281DRAFT_02410 at 584.069 kb on - strand at 584.145 kb on + strand, within H281DRAFT_02410 at 584.145 kb on + strand at 584.145 kb on + strand, within H281DRAFT_02410 at 584.145 kb on + strand, within H281DRAFT_02410 at 584.146 kb on - strand, within H281DRAFT_02410 at 584.252 kb on - strand at 584.287 kb on + strand at 584.311 kb on + strand at 584.312 kb on - strand at 584.345 kb on + strand at 584.346 kb on - strand at 584.370 kb on + strand at 584.401 kb on - strand at 584.735 kb on + strand, within H281DRAFT_02411 at 584.746 kb on + strand, within H281DRAFT_02411 at 584.887 kb on + strand, within H281DRAFT_02411 at 584.888 kb on - strand, within H281DRAFT_02411 at 584.946 kb on - strand, within H281DRAFT_02411 at 585.160 kb on + strand, within H281DRAFT_02411 at 585.160 kb on + strand, within H281DRAFT_02411 at 585.160 kb on + strand, within H281DRAFT_02411 at 585.161 kb on - strand, within H281DRAFT_02411 at 585.164 kb on - strand, within H281DRAFT_02411 at 585.164 kb on - strand, within H281DRAFT_02411 at 585.379 kb on + strand at 585.379 kb on + strand at 585.574 kb on + strand at 585.731 kb on + strand at 585.731 kb on + strand at 585.731 kb on + strand at 585.732 kb on - strand at 585.732 kb on - strand at 585.891 kb on + strand at 586.088 kb on - strand, within H281DRAFT_02414 at 586.090 kb on + strand, within H281DRAFT_02414 at 586.091 kb on - strand, within H281DRAFT_02414 at 586.215 kb on + strand, within H281DRAFT_02414 at 586.243 kb on - strand, within H281DRAFT_02414 at 586.316 kb on + strand at 586.316 kb on + strand at 586.324 kb on + strand at 586.324 kb on + strand at 586.325 kb on - strand at 586.325 kb on - strand at 586.375 kb on + strand at 586.375 kb on + strand at 586.381 kb on + strand at 586.381 kb on + strand at 586.383 kb on + strand at 586.383 kb on + strand at 586.383 kb on + strand at 586.383 kb on + strand at 586.383 kb on + strand at 586.384 kb on - strand at 586.447 kb on + strand at 586.447 kb on + strand at 586.447 kb on + strand at 586.447 kb on + strand at 586.448 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 583,357 - H281DRAFT_02409 0.87 -0.3 583,357 - H281DRAFT_02409 0.87 -0.7 583,357 - H281DRAFT_02409 0.87 -1.2 583,366 - H281DRAFT_02409 0.87 -1.3 583,391 - H281DRAFT_02409 0.88 -0.7 583,422 + H281DRAFT_02409 0.89 -0.5 583,422 + H281DRAFT_02409 0.89 -0.4 583,423 - H281DRAFT_02409 0.89 -0.9 583,423 - H281DRAFT_02409 0.89 -1.2 583,423 - H281DRAFT_02409 0.89 -0.2 583,441 - H281DRAFT_02409 0.90 -0.9 583,441 - H281DRAFT_02409 0.90 -1.9 583,507 + -0.5 583,508 - -0.7 583,884 + +0.0 583,884 + -0.7 583,884 + -1.1 583,884 + -0.8 583,884 + -0.7 583,885 - -0.6 583,886 + +0.6 583,886 + +1.7 583,886 + -0.4 583,886 + -1.7 583,887 - +0.5 583,887 - -0.6 583,887 - +0.1 583,919 - +0.1 583,990 + H281DRAFT_02410 0.25 -0.6 583,991 - H281DRAFT_02410 0.25 -0.9 584,068 + H281DRAFT_02410 0.45 -0.6 584,068 + H281DRAFT_02410 0.45 -1.9 584,069 - H281DRAFT_02410 0.45 -1.5 584,069 - H281DRAFT_02410 0.45 -0.3 584,069 - +2.7 584,145 + H281DRAFT_02410 0.65 -2.5 584,145 + +0.3 584,145 + H281DRAFT_02410 0.65 +0.6 584,145 + H281DRAFT_02410 0.65 -0.5 584,146 - H281DRAFT_02410 0.66 -0.8 584,252 - -0.7 584,287 + -0.6 584,311 + -1.6 584,312 - +0.3 584,345 + -1.1 584,346 - -0.3 584,370 + -1.1 584,401 - +0.0 584,735 + H281DRAFT_02411 0.34 -1.4 584,746 + H281DRAFT_02411 0.35 -1.1 584,887 + H281DRAFT_02411 0.48 -1.0 584,888 - H281DRAFT_02411 0.48 -0.8 584,946 - H281DRAFT_02411 0.53 -0.9 585,160 + H281DRAFT_02411 0.72 -1.2 585,160 + H281DRAFT_02411 0.72 -0.9 585,160 + H281DRAFT_02411 0.72 -0.8 585,161 - H281DRAFT_02411 0.72 -1.3 585,164 - H281DRAFT_02411 0.72 -0.3 585,164 - H281DRAFT_02411 0.72 -1.4 585,379 + +1.7 585,379 + -0.2 585,574 + -1.7 585,731 + -0.5 585,731 + -0.2 585,731 + -1.8 585,732 - +1.0 585,732 - -2.1 585,891 + -1.7 586,088 - H281DRAFT_02414 0.45 +0.3 586,090 + H281DRAFT_02414 0.46 -0.7 586,091 - H281DRAFT_02414 0.46 +1.0 586,215 + H281DRAFT_02414 0.81 +0.1 586,243 - H281DRAFT_02414 0.89 -0.3 586,316 + -0.0 586,316 + -0.3 586,324 + +0.1 586,324 + -0.6 586,325 - +1.8 586,325 - -0.1 586,375 + +1.2 586,375 + +1.2 586,381 + +0.3 586,381 + +0.3 586,383 + +0.0 586,383 + +0.1 586,383 + +0.2 586,383 + -0.4 586,383 + +0.1 586,384 - +1.1 586,447 + +0.2 586,447 + +0.5 586,447 + -0.2 586,447 + +0.5 586,448 - -1.1
Or see this region's nucleotide sequence